This study was conducted to analyze the operating conditions of predenitrification process to improve the treatment efficiency in low organic loading sewage plant in use today, and to investigate the treatment efficiency of pilot plant added night soil as well as the nitrogen removal characteristics of pilot plant added carbon sources. In the operation under the condition of BOD5 sludge load 0.03-0.28kg BOD5/kg VSS/d and oxic ammoniac nitrogen sludge load 0.02-0.24 kgNH4+-N/kg MLVSS/d, nitrification efficiency is higher than 95%. In order to achieve 70% nitrogen removal at the T-N sludge loading 0.06kg T-N/kg VSS․d and the SRT 6~11 days, optimum operating factors were revealed to CODcr/T-N ratio 9, recycle ratio 2.6, and denitrification volume ratio 0.33. At this time, denitrification capacity was approximately 0.09 kg NO3--N/kg CODcr; specific nitrification rate was 3.4mg NH4+-N/g MLVSS/hr; and specific denitrification rate was 4.8mg NO3--N/g MLVSS/hr.