Background: Hamstring shortness (HS) contributes to the limit of hip and knee range of motion and can limit the execution of multi-segmental movement exercises such as squatting, thereby altering the muscle activation. The double leg squat (DS) is fundamental exercise for knee injury rehabilitation programs. However, it may aggravate knee joint injury and cause low back pain. The Bulgarian split squat (BSS) is a one-leg squat exercise with greater knee joint stability than DS. Measuring muscle activation during the squat will provide insight into the contribution of hamstring activation and quadriceps and hamstring (Q:H) ratio to the knee in participants with HS. Objects: This study compares hamstring activation and Q:H ratios during squat exercises in individuals with and without HS. Methods: This study classified 30 healthy participants, based on active knee extension, into two groups: with HS and without HS. Surface electromyography was performed to determine muscle activation of the medial hamstring (MH) and biceps femoris of the hamstrings and coactivation of the Q:H. All participants performed DS and BSS. Results: MH activity was significantly higher in the with HS group than in the without HS group during all exercises, and the Q:H ratio was significantly closer to 1.0 during BBS compared to DS. Additionally, the with HS group had a significantly lower Q:H ratio than without HS group. Conclusion: In comparison between exercises, BBS can bring the Q:H ratio closer to 1.0 compared to DS, which may enhance knee stability. However, in between-group comparisons, the increased tension due to the shortening of the hamstrings leads to heightened hamstring activation, resulting in a Q:H ratio that is closer to 1.0 compared to the without HS group. Therefore, relying solely on the Q:H ratio to assess knee stability may be risky.

Background: Robot-assisted gait training (RAGT) is an effective method for walking rehabilitation. Additionally, the body weight support (BWS) system reduces muscle fatigue while walking. However, no previous studies have investigated the effects of RAGT with BWS on isokinetic strength of quadriceps and hamstring muscles. Objects: The purpose of this study was to investigate the effects of torque, work, and power on the quadriceps and hamstring muscles during RAGT, using the BWS of three conditions in healthy subjects. The three different BWS conditions were BWS 50%, BWS 20%, and full weight bearing (FWB). Methods: Eleven healthy subjects (7 males and 4 females) participated in this study. The Walkbot_S was used to cause fatigue of the quadriceps and hamstring muscles and the Biodex Systems 4 Pro was used to measure the isokinetic torque, work, and power of them. After RAGT trials of each of the three conditions, the subjects performed isokinetic concentric knee flexion and extension, five at an angular velocity of 60°/s and fifteen at an angular velocity of 180°/s. One-way repeated analysis of variance was used to determine significant differences in all the variables. The least significant difference test was used for post-hoc analysis. Results: On both sides, there were significant differences in peak torque (PT) of knee extension and flexion between the three BWS conditions at an angular velocity of 60°/s and 180°/s conditions. A post-hoc comparison revealed that the PT in the BWS 50% was significantly greater than in the BWS 20% and the FWB and the PT in the BWS 20% was significantly greater than in the FWB. Conclusion: The results of this study suggest that the lower BWS during RAGT seems to lower the isokinetic torque, work, and power of the quadriceps and hamstring muscles because of the muscle fatigue increase.