Microbial parameters of indoor air containing systematic, random, and accidental error were analyzed statistically by the water quality control guidelines to suggest a guideline of indoor air. Samples were collected from 20 subway stations of Seoul Metropolitan City. Systematic errors resulted from sampling devices were checked and removed by using same devices and flow rate check. Another source of systematic error resulted from different testing personals were assessed and qualified by proficiency test. Increase of replicate sample numbers could minimize random errors and use of geometric mean instead of arithmetic mean gave more statically reliable representative values for microbial parameters. Considerations for the culture media, colony counting results with incubation time, and statement of supplemented statistical data were also suggested.