Origin of the red imported fire ant (RIFA, Solenopsis invicta) is South America, a tropical climate region. It has settled in the invasive area, causing various problems. In recent, colonies of Solenopsis invicta were discovered in Busan in 2017, and then in Busan, Pyongtaek, Incheon, Daegu and Ansan in 2018. This study aims to validate 31 microsatlite loci referred from previous study for tracing origin of Solenopsis invicta colonies. We tried to test previously developed microsatellite loci so that we establish the protocol for molecular epidemiology of RIFA. We applied 31 microsatellite loci to DNA samples from the RIFA colonies found in Texas and Florida, USA. We will present 31 markers were divided into three injections and the amplification rate for each marker was shown as follows.

Red imported fire ant Solenopsis invicta is one of the 100 most threatening alien species in the world. Originally distributed in parts of South America, it has invaded 24 other countries and regions now. Because of its ferocious habits, rapid reproduction and strong competitiveness, it is easy to outbreaks and cause disaster in a short period of time after the invasion, and will endanger agriculture and forestry, human health, biodiversity and public safety. The fire ant had been found in Taoyuan, Taiwan in September 2003, and Guangdong, mainland China on September 23, 2004. As a new invasive species in south China, although it was paid serious attention by the governments and the scientists, but the fire ant has kept spreading fast after its invasion. In order to delay its expansion and control its infestation, the following research on the fire ant has been carried out and completed 14 years after its invasion in China. To discover the fire ant invasion in mainland China, and reveal its spreading and expanding law, including 1) the genetic diversity, invasive sources, ways, routes, migration and expansion of the fire ant in China at different spatial scales; 2) a system of methods for estimating invasion and expansion; 3) monitor continuously for 14 years to clarify its diffusion dynamics in mainland China. Clarify the catastrophic law and mechanism of the fire ant invasion in mainland China, and include 1) The laws and mechanisms of invasive biology, behavior, population dynamics, ecological adaptation, social immunity, interspecific competition/reciprocity; 2) The disturbance of the fire ant invasion to crops, species relationship, community structure, control pest function, pollination function and soil environment in southern six ecosystems; 3) The laws and mechanisms of inhibiting invasion and restoring diversity in the diverse habitats; 4) New functions of chemosensing related genes and the regulation mechanism of light avoidance behavior. Reveal the basic theory of chemical control of the fire ant, and establish a safe and efficient technical system for emergency control and eradication, such as 1) the transmission toxicity, behavioral toxicity, reproductive disturbance, repellent activity, control effect and environmental effect of 46 insecticides; 2) pesticide-induced killing-queen behavior and its mechanism; 3) standard methods for evaluation of control effect for insecticides. Reveal the basic theory of chemical control of the fire ant, and establish a safe and efficient technical system for emergency control and eradication, such as 1) Five safe and efficient formations and their application technologies, and breakthroughs in high attractiveness, waterproofing formations and application methods, and the control effect of single use with 89% - 97%; 2) emergency prevention and control in 8 types of ecological areas eradication mode and technology system, with the breakthrough of inefficient or ineffective products and technology in rainy and low temperature seasons, and the control effect with 96% - 100%, and local eradication of 19 areas. Put forward risk management strategies, and build up an accurate, efficient and safe monitoring and quarantine technology system, and include 1) the suitability and risk management strategies and techniques based on more extensive research; 2) new monitoring technologies with the accuracy of 98% - 100%; 3) relationship among the three methods, and a monitoring and index system; 4) efficient quarantine technology for entry-exit, occurrence regions and transportation; 5) near infrared reflection ant nest detection system, and information management system; 6) construct the management, technical standards and promotion system, and apply them widely; 7) more than 40 national standards and schemes in a management/technical standard system.