The recycling cell formation problem means that disposal products are classified into recycling product families using group technology in their end-of-life phase. Disposal products have the uncertainties of product condition usage influences. Recycling cells are formed considering design, process and usage attributes. In this paper, a new approach for the design of cellular recycling system is proposed, which deals with the recycling cell formation and assignment of identical products concurrently. Fuzzy ART neural networks are applied to describe the condition of disposal product with the membership functions and to make recycling cell formation. The approach leads to cluster materials, components, and subassemblies for reuse or recycling and can evaluate the value at each cell of disposal products. Disposal refrigerators are shown as an example.

The recycling cell formation problem means that disposal products are classified into recycling part families using group technology in their end of life phase. Disposal products have the uncertainties of product status by usage influences. Recycling cells are formed considering design, process and usage attributes. In this paper, a novel approach to the design of cellular recycling system is proposed, which deals with the recycling cell formation and assignment of identical products concurrently. Fuzzy clustering algorithm and Fuzzy-ART neural network are applied to describe the states of disposal product with the membership functions and to make recycling cell formation. This approach leads to recycling and reuse of the materials, components, and subassemblies and can evaluate the value at each cell of disposal products. Application examples are illustrated by disposal refrigerators, compared fuzzy clustering with Fuzzy-ART neural network performance in cell formation.

large scale production of cloned embryos requires the technology of multiple generation nuclear transplantation(NT) using NT embryos as the subsequent donor nuclei. The purposes of this study were producing the second generation cloned rabbit embryos, and also to determine the electrofusion rate and in vitro developmental potential comparatively in the cloned embryos of the first and second NT generation. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FCS) at 47 hours after hCG injection In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gi /S transition of 32-cell stage. The first generation NT embryos which were developed to 8-cell were synchronized in Gi /S transition phase of the following 16-cell stage and used as donor nuclei for second generation Synchronization of the cell cycle of blastomeres was induced, first, using an inhibitor of microtuble polymerization, colcemid for 10 hours to arrest blastomeres in M phase, and secondly, using a DNA synthesis inhibitor, aphidicolin for 1.5 to 2 hours to arrest them in Gi /S transition boundary. The recipient cytoplasms were obtained by removing the nucleus and the first polar body from the oocytes collected at 14 hours after hCG injection. The separated donor blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 sec at 1.25 kV /cm in 0.28 M rnannitol solution The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39 in a 5% incubator. Following in vitro culture of the first and second generation cloned embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The results obtained were summarized as follows: 1. The electrofusion rate was found to be similar as 79.4 and 91.5% in the first and second generation NT rabbit embryos, respectively. 2. The in vitro developmental potential to blastocyst stage of the second generation NT embryos (23.3%) was found significantly(p<0.05) lower, compared with that of the first generation NT embryos (56.8%). 3. The mean blastomeres counts of embryos developed to blastosyst stage following in vitro culture for 120 hours and also their daily cell cycles during the culture period were decreased significantly (p<0.05) to 104.3 cells and 1.33 cylces in the second NT generation, compoared with 210.4 cells and 1.54 cycles in the first NT generation, respectively.