This study analyzed business ecosystems in 4 case regions(Yeongdong, Seocheon, Sunchang, and Hadong) that carry forward the regional 6th industrialization. The analysis aimed to draw implications to build a healthy ecosystem for the sustainable development of the regional 6th industrialization. As a result of the analysis, 4 districts show different characteristics for their growth stages, and these results provide implications for policy directions for the development of the regional industry. The following basic direction was set for the sustainable regional 6th industrialization based on the healthy ecosystem. First, policy support should be differentiated by considering each region’s growth stage. Second, to improve the regional innovation capacity, it is needed to lay the foundation continually and strengthen diverse support for it. Third, a stronger and efficient implementation system is necessary for the regional 6th industrialization.