This study investigates the optimization of sectional shape with two dimensions on the rubber gasket of electric vehicle battery in order to maintain the airtightness and watertightness. For the section optimization, the shape of protruding section was analyzed as design variables and the design point was composed by the design of experiment(DOE) for the selected protruding shape. The uniaxial tensile test was carried out for the analysis of rubber gasket and five parameters of Mooney-Rivlin hyperelastic model were derived from the test data in order to construct the strain energy function for nonlinear behavior. The rubber gasket compression analysis was performed by using ANSYS of a commercial software and the performance of optimal shape was verified by performing the tests of watertightness and airtightness on the 3D rubber gasket with the derived section.