Plant essential oils (EOs) exhibit an array of biological activities against insect pests. However, their negative influences on the pheromonal activity of azuki bean beetle (ABB), Callosobruchus chinensis L., the field-to-storage pest of legumes, have not received research attentions. We evaluated the effects of three EOs and their two major components on the attractiveness of male ABBS to synthetic homofarnesal (2E- : 2Z-homofarnesal = 6:4) using Y-tube olfactometry in laboratory and rocket traps in the semi-open vinyl house. The tested EOs of Illicium verum, Croton anisatum and Gaultheria fragrantissima as well as the major components, trans-anethole and methyl salicylate ascertained significant negative effect against homofarnesal both in Y-tube olfactometry and in semi-open vinyl house tracing out a new opportunity of integrating them in ABB management programs both in field and storage.

Ethyl formate (EF) and methyl salicylate (MS) are naturally occurring and generally safe substances for use. Sub-lethaldoses of EF and MS were evaluated to find synergistic fumigation action against azuki bean beetle (ABB), Callosobruchuschinensis (L.). Susceptibility of the ABB to EF was in the order of eggs =adults >larvae >pupae. Adults was most sensitiveto MS, and followed by eggs. Larvae and pupae were highly tolerant to MS. However, in synergistic effects of EF andMS showed that all the stages of ABB were highly sensitive in the order of adults >eggs >larvae >pupae. Highest synergisticratio was found against adults when using LC10 of both EF and MS as compared to other stages. Notably, MS synergizedthe EF exposure to all stages of ABB. This finding paves the way to exploiting synergistic activity of MS and EF againstABB for the safeguard of stored azuki bean grains.

식물의 저항성과 방어관련물질 유도에 관련된 salicylate가 담배 미분화세포로부터 분리된 isoperoxidase A3의 활성에 미치는 영향을 살펴보고, Fe2+ 의 첨가에 의하여 salicylate에 의한 효소활성 억제가 보호되는 양상을 조사하였다. 다양한 농도의 salicylate가 isoperoxidase A3의 활성에 미치는 영향을 조사한 결과 0.48mM salicylate에 의하여 isoperoxidase A3의