This study presents the results of an experimental program carried out for estimating the variation of the flexural rigidity of reinforced concrete beams with different shear span-to-depth ratios. The specimens are designed to have identical sectional dimension, tension and compression reinforcement ratios, and material properties, except shear span-to-depth ratio. Experimental results showed that the flexural rigidity of reinforced concrete beams was affected by the shear span-to-depth ratio of specimens.