The National Environmental Specimen Bank (NESB) has set up a plan to develop reference materials in the facility for assuring analytical quality and validating analytical methods for its monitoring samples. Some of the crucial characteristics that reference materials must consist of are homogeneity and stability of both intra and inter-bottles. In this study, we examined the homogeneity and stability of cryogenically-milled muscle samples, from Common Carp (Cyprinus carpio) for total mercury. Homogeneity was tested using ANOVA analysis and regression analysis was used to test short-term stability. The variations of total mercury concentration did not significantly differ between the intra and the inter-bottle (F=0.8, p=0.37). Additionally, relative standard deviation of the total mercury concentration showed low values (2.28%). For the short-term stability test, total mercury variations were not statistically significant as demonstrated by the result of the regression analysis (F ratio = 3.11, p = 0.18). This suggests that the cryogenic-milling process has statistically proven the degree of homogeneity and short-term stability for samples of carp muscles in the chemical analysis for total mercury.