Cracking of slabs will be caused by applied load and volume changes during the life of a structure and thus it reduces flexural stiffness of slabs. The effect of slab cracking must be considered for appropriate modeling of the flexural stiffness for frame members used in structural analysis. Analytical and experimental study was undertaken to estimate the stiffness reduction of slabs In the analytical approach, the trend of slab stiffness reduction related to gravity and lateral loads is found and the stiffness reduction factor ranged from a half to a quarter in ACI building code is reasonable when defining range. Analyzing results of the test by Hwang and Moehle for 0.5% drift show that the differences of rotational stiffness on the connection types is found and good results of lateral stiffness using the value of one-third is obtained. To evaluate the stiffness reduction factor of one-third for residential building in domestic regions, the test is performed