The stable fly, Stomoxys calcitrans L., is an important pest of livestock. Stable flies are considered as mechanical vectors of veterinary disease. Pyrethroids and organophosphates have been widely used for stable fly control. To establish resistance monitoring molecular tool, we isolated the partial cDNA and genomic fragments of voltage-sensitive sodium channel and acetylcholinesterase genes encompassing the well known conserved sites for resistance-associated mutations. To examine the current status of stable fly resistance to pyrethroids and organophosphates mediated by the nerve insensitivity mechanism in Korean population of S. calcitrans, DNA-based genotyping in conjunction with residual contact vial (RCV) bioassay were conducted with 11 representative regional field populations. No resistance-associated mutations were detected in these S. calcitrans populations, suggesting that these populations are likely still susceptible to both pyrethroids and organophosphates. Establishment of RCV bioassay protocol and availalbility of target site sequence information will greatly facilitate resistance monitoring of S. calcitrans in the field.

1. 침파리의 동계사육 결과 평균온도 에서 유충기간 7일, 용기간 9.2일, 전산란기간 14.2일, 성충수명 23.3일이었다. 2. 5월부터 7월까지 사육온도 로 고정시켰을 때의 생육기간은 유충기간 8.0일, 용기간 5.3일, 전산란기간 11.7일, 성충수명 30.1일 이었다. 3. 용 개체중량은 5월-7월의 에서 13.3mg으로서 현저한 증가를 볼 수 있었다. 4. 전산란 기간중의 성충사망율은 습도 조절(R. H.)에 따라서 큰 차이를 보여주었으며 누대사육을위해서는 의 R.H.를 유지할 필요가 있을 것으로 본다. 5. 성충의 산란율은 산란 시작후 제8-10일에 제일 높다.