Dengue is the most important arboviral disease in tropical and subtropical areas where 2.5 billion people are at risk of infection. Aedes albopictus will be a major vector transmitting dengue virus in Korea, where this virus overseas inflow is possible. We collected mosquitoes in Jeju, Busan, Gunsan, and Incheon using BG sentinel trap from April to October in 2016. Collected mosquitoes were conducted virus detection using real-time PCR method and analysis bloodmeal source of Ae. albopictus. A total of 15 species comprising 7 genera were identified and 4,854 female mosquitoes collected. The most dominant species ratio (SR) was 52.9% (Culex pippins complex) followed by 20.3% (Ae. albopictus), 10.8% (Ae. vexans nipponi) and Ochlerotatus dorsalis (3.8%). Dengue virus was not detected. Bloodmeal source of Ae. albopictus was mammals (80.9%) followed by birds (18.6) and amphibians (0.5).

The purpose of this monitoring is to survey the geographical distribution of tick species using dry ice bait traps and flagging methods at each ten provinces (GangwonⅠ,GangwonⅡ, Gyeonggi, Chungbuk, Chungnam, Gyeongbuk, Gyeongnam, Jeonbuk, Jeonnam and Jeju area) and one Metropolitan area in the Republic of Korea for eight months from April through November, 2016. A total of 65,339 ixodid ticks (8,200 females, 1,988 males, 31,453 nymphs and 23,698 larvae) was collected, belonging to three genera (Haemaphysalis, Ixodes and Amblyomma). Haemaphysalis longicornis was the most commonly collected species, which is represented for 96.88% of all the collected ticks and followed by H. flava (2.69%), I. nipponensis (0.35%), A. testudinarium (0.05%) and H. japonica (0.03%) in the Republic of Korea for the study period. Haemaphysalis longicornis was a dominant species observed in these eleven areas.

Dengue is the most important arboviral human infection worldwide. Approximately 50-100 million cases occur annually and more than 2.5 billion people are at risk of infection. This virus is possibility of overseas inflow to be high in Korea. We collected mosquitoes in Jeju, Busan, Gunsan, and Incheon using BG Sentinel trap from April to August in 2016. Collected mosquitoes were conducted virus detection using real-time PCR method. A total of 15 species comprising 7 genera were identified and 3,696 female mosquitoes collected. The most dominant species ratio (SR) was 49.4% (Culex pippins complex) followed by 21.8% (Aedes albopictus), 13.6% (Ae. vexans) and Ochlerotatus dorsalis (4.8%). Dengue virus was not detected in any mosquitoes until now.

West Nile Fever is one of most serious disease spreading throughout world since 1990. West Nile Virus (WNV) is transmitted by infected mosquitoes. The possibility of overseas inflow of this virus seems to be high in Korea. To know the WNV infection of mosquitoes, we collected mosquitoes in Jeju, Busan, Gunsan, and Incheon using CDC light trap and BG Sentinel trap from April to August in 2016. Collected mosquitoes were conducted virus detection using real-time PCR method. A total of 22,632 mosquitoes representing 9 genera and 18 species were collected. The most dominant species was Culex pippins complex (36%) followed by Aedes vexans (13%), Ae. albopictus (10%) and Ochlerotatus koreicus (10%). WNV was not detected in any mosquitoes until now.

This study was conducted to evaluate a visual surveillance system. The advancement of recording technology and network service make it easy to record and transfer the videos. Moreover, progressed recognition technology help to make a distinction each other. Cows show distinguishing behaviors during their estrus period. The mounting is one of the behaviors. The result was different depending on the breed of the cows and the size of the farm. In the case of Korean native cattle, the estrus detection rate was 71.15%, however, dairy cows, the estrus detection rate was 39.38%. At the farms having below 6 modules, the estrus detection rate was 87.41%. On the other hand, at the farms having over 6 modules, the estrus detection rate was 77.78%. With the proper progress, the visual surveillance system can be used to detect heat detection.

A surveillance of chigger mites was performed to monitor the incidence of scrub typhus vectors at 4 environmental collection points of 6 locations from September to November 2014 in Korea. During the survey period, 420 chigger mites were collected and the dominant species was Leptotrombidium scutellare (42.6%). The first appearance of chigger mite was at 37th week (9.3.-9.10.) and the collected numbers of chigger mites was the highest at 43rd week (10.17.-10.23.). In Goryeong-gun, 299 chigger mites were collected, whereas 5 chigger mites were collected In Yesan-gun. The high environmental collecting rates were recorded at rice field (56%) and waterway (20%). The annually collected numbers (2012-2014) of chigger mites were compared with the average temperatures in August. This result suggests that the average temperature in August might be related with the annual incidence of scrub typhus vectors in Korea. However, the relationship between climate factors and the density of chigger mites needs to be studied by long-term periodical surveillance.

To clarify the geographical distribution of scrub typhus vectors in Korea, the first survey of chigger mites was conducted from 2005 to 2007 by collecting wild small mammals twice a year (spring and autumn) at 24 sites nationwide. The two predominant mite species were Leptotrombidium pallidum (52.6%) and L. scutellare (27.1%). However, the proportions of L. scutellare in southern areas, including endemic provinces such as Chungcheongnam-Do, Jeollabuk-Do, Jeollanam-Do, and Gyeongsangnam-Do, were relatively higher than in central Korean regions where L. pallidum was predominant. In autumn, the ratio of L. scutellare increased to 42% while the ratio of L. pallidum decreased. The geographical distribution map of the L. scutellare chigger index was identical to the incidence pattern of scrub typhus, whereas those of overall mites and L. pallidum showed no relationship with case incidence patterns. Distribution mapping analysis shows an identical geographical distribution of L. scutellare and epidemic incidence of scrub typhus in South Korea. The second periodical survey was performed from 2011 to 2013. The result suggests that the distribution of L. scutellare has not been changed remarkably in comparison to the first survey.

There are over 3,000 different species of mosquitoes throughout the world; currently 56 species are reported in Korea. Mosquitoes can act as vectors for many disease-causing viruses and parasites during blood sucking. Various species of mosquitoes are estimated to transmit various types of disease to more than 700 million people annually in Africa, South America, Central America, Mexico, Russia, and much of Asia, with millions of resultant deaths. At least two million people annually die of these diseases, and the morbidity rates are many times higher still. To prevent the spread of diseases, KNIH (Korea National Institute of Health) used three categorized methods; identifying or taxonomic analysis of mosquitoes, detecting virus caring mosquitoes, and detecting malaria from Anopheline mosquitoes. We have proved that taxonomic analysis using DNA barcording method (COI gene) is useful to complement identification of mosquito species. In detecting virus, we have reported Cx. orientalis as a new potential Japanese encephalitis virus (JEV) vector. We also reported that the Anopheline mosquito species composition and Plasmodium vivax infection rates in malaria hot spot in Korea.

Climate change by global warming is predicted to affect on public health including increasing incidence of vector borne diseases. Vector borne diseases are transmitted by arthropod vectors, such as mosquitoes, chigger mites and ticks, and are highly sensitive to climate changes. The surveillance and R&D of infectious disease vectors are becoming important for climate change preparedness in Korea. So far, 10 regional vector surveillance centers (Incheon, Gyeonggi, Gangwon, Chungbuk, Chungnam, Jeonbuk, Jeonnam, Gyeongbuk, Gyeongnam, and Jeju) have been established to monitor vectors and their pathogens against endemic diseases (japanese encephalitis, malaria, scrub typhus and SFTS) and imported diseases (dengue fever, west nile fever and yellow fever). The information on geographical distribution as well as real-time monitoring of vectors and their pathogens will be presented by this surveillance system, ‘VectorNet’. The R&D plan on vectors and their pathogens is now discussing in government-wide R&D committee on infectious diseases. The R&D areas will be included: 1) establishment of nationwide monitoring system, 2) study on biological and molecular characteristics, 3) development of vector identification and pathogen diagnosis methods, 4) development of vector control techniques, 5) management and conservation of vector resources.

The Korean National Residue Program (KNRP) use three plans for the sampling of domestic meats: monitoring, surveillance, and exploratory testing. We analyzed the results of monitoring and surveillance for residual veterinary drugs and pesticides in meat during 2012 and 2013. KNRP targets food-producing animals including cattle, pigs, chickens, ducks, sheep (goats), and horses. Sampling mainly focuses on veterinary drugs and pesticide residues found in tissues such as muscle, kidney, and liver. A total of 548 residue violations occurred between 2012 and 2013, mainly in pigs (427 violations) and cattle (113 violations). The most commonly found compounds were antimicrobials such as penicillins, fluoroquinolones, aminoglycosides, tetracyclines, sulfonamides, and macrolides. Pig residue violations were mainly for the presence of penicillins (34%) and fluoroquinolones (28%), while most residue violations in cattle were due to the presence of penicillins (32%) and aminoglycosides (27%). The overall rates of violations were 0.21% in 2012 and 0.10% in 2013. A major cause of violations was the failure to follow the appropriate withdrawal periods (68.8%). The results of the KNRP were analyzed to provide information on agricultural chemical residues that are of public health concern and to help control and prevent residue violations for ensuring food safety.

S. aureus is reported as a major cause of nosocomial infections after dental care and involved in endocarditis, bacteremia, osteomyelitis, peritonitis, and soft tissues etc. It is very important to identify the distribution and the diversity of toxin gene associated with the S. aureus expression in dental care patients with periodontitis directly for an effective prevention and treatment of dental diseases. Fifty four strains of S. aureus were isolated from the saliva of 129 patients who were diagnosed with periodontitis at dental clinics and hospitals located in Seoul. The distribution of the virulence gene and the genetic diversity of the strains were studied using the polymerase chain reaction with isolated strains. The enterotoxin test showed Seb was the most frequent gene with 88.9%. The hemolysin gene of Hla, Hib and Hld were the most frequently gene with 98.1% (53 strains), leukocidins gene of lukM showed 90.7% (49 strains), and laminin binding protein gene of Eno showed 100% (54 strains), respectively. The diversity of the enterotoxin gen was held as Seb-Seg-Sei gene of 35.2% (19 strains), the diversity of hemolysin gene of Hla-Hlb-Hld gene was 98.1% (53 strains) and the diversity of leukocidins gene of LukD-LukM were 88.9% (48 strains), respectively. Patients with dental disease showed somehow high toxin gene expression so that S. aureus in dental care area is judged to show very highly pathogen with a high and infection rate. In the future, additional studies for these toxin genes seem to be required.

Pancreatic cancer is still one of the most dreadful malignancies with 5-year survival of 5%. The lack of effective diagnostic tools for early detection of pancreatic cancer is the major factor for the poor prognosis of pancreatic cancer. Considering the relatively lower incidence of pancreatic cancer, it seems to be reasonable to find and investigate the high risk group of pancreatic cancer rather than to screen general population. During the last 2 decades, several epidemiologic and genetic high risk groups of pancreatic cancer were found. Especially, western countries including US have been taking care of genetic high risk group and reported several meaningful outcomes. With advances of understanding molecular carcinogenesis and progression of pancreatic cancer, the effort to find specific biomarkers for both detection and treatment of pancreatic cancer has been overwhelmed. Various types of biomarkers including protein, microRNA, exosomes or circulating tumor cells itself have been under investigations. Although there has been no single biomarker which can overcome CA19-9 in serum to date. It will be worthwhile to wait for more potent biomarker which can be used for early diagnosis and treatment of pancreatic cancer in near future.

The purpose of this article is to search stakeholders' recognition related to Fire Construction Surveillance system; a client(order), fire fighting officer, supervision company, construction company, design company, other. The paper provides 4 important things. Firstly, a client(order) suggested the lowest recognition in the needs about the need of separation order, that of contract for any construction work limited registered companies, and that of standard price for engineering.. Secondly, the design company is lowest recognition in guidance and control participating in construction-design phase. Thirdly, the design company is lowest in adjustment about manpower-allocation according to allocation criteria of technical manpower. Fourthly, a client(order) and supervision company are relatively lower than other stakeholder in necessity on special education and training system for auditor in fire protection construction. Almost all related matters are related to more or less stakeholders' interest.

This study describes the national program of year-round surveillance and monitoring for avian influenza (AI). The validity of the epidemiologically-based surveillance scheme was assessed. Korea’s current surveillance program is aimed at detecting subclinical infection of either the highly pathogenic avian influenza (HPAI) virus or the low pathogenic avian influenza virus, types H5 and H7, both of which carry risk of converting to HPAI. The current AI surveillance program has demonstrated that implementing a surveillance strategy is plausible. Farmer and livestock related professional support is the critical step of specimen collection to discover hidden infection. Early detection of AI virus infection can achieve best by the combined efforts of farmers, animal health authorities, and other related industries.

Vector-borne diseases, including those transmitted by ticks, pose serious public health threats to US military populations, as well as military and civilian populations in the Republic of Korea. (1) From 2004-2010, a total of 54,495 ticks belonging to three genera and eight species [Haemaphysalis longicornis (33,242; 61.0%), H. flava (18,525; 34.0%), Ixodes nipponensis (2,420; 4.4%), H. phasiana (216, 0.4%), H. japonica (33; <0.1%), Amblyomma testudinarium (26; <0.1%), I. turdus (17; <0.1%), and I. persulcatus(16; <0.1%)] were collected by tick drag. (2) As part of the rodent-borne disease surveillance program, a total of 6,773 ticks belonging to two genera and four species were collected from small mammals at US military installations and training sites in Korea from 2004-2009. (3) In collaboration with Seoul National University, a total of 920 ticks, belonging to two genera and six species were collected from small-large mammals. (4) In addition, a total of 250 ticks belonging to two genera and five species were collected from migratory birds. (5) I. simplex and I. vespertilionis also were collected from limited numbers of bats. Additional collections from small-large mammals, migratory birds, bats, and other animals are needed to identify the geographical and host range of ticks and the associated pathogens they harbor. Furthermore, these data can be used to provide predictive emergence and distributions of ticks and their associated pathogens.

A surveillance of chigger mites was conducted to clarify the incidence of scrub typhus vectors by new chigger mite collecting traps instead of trapping wild rodents for surveillance from each 4 collection points of 5 regions from September to November 2012 in Korea. During the surveillance period, 2,829 chiggers were collected and 10 species of 4 genera were identified. The first appearance of chigger mite and patients was 39 week (9.19~9.25) and the density of chigger mites had the peak in 43 week (10.17~10.23) and the density of patients had the peak in 44 week (10.24~10.30), respectively. In Goryeong-gun, a total of 1,797 mites representing 4 genera and 6 species were collected and the predominant species were Leptotrombidium scutellare (86.0%), L. pallidum (6.5%) and L. palpale (4.7%) whereas, In Gurye-gun, a total of 89 mites representing 2 genera and 3 species were collected and the predominant species were L. scutellare (46.5%) and L. palpale (36.2%) in similar to the natural environment. The high collecting rates were recorded at rice field (71.8%) and waterway (11.6%). This result shows that the surveillance of scrub typhus vectors by new chigger mite collecting trap is useful as an eco-friendly method.

As veterinary medicine industry has grown up steadily, the government quality control of veterinary medicine in market has been performed since 1964. The result of post-market surveillance assay from 2009 to 2012 was analyzed in this study. The average violation rate of all veterinary medicines, was 3.2%, 2.8%, 2.8% and 1.4% in 2009, 2010, 2011 and 2012, respectively. The total average violation rate in the category of antibiotics, other drugs except antibiotics, biologics were 1.2%, 4.4% and 0.8%, respectively. The violations due to insufficiency of major active ingredient content occupied 76.7% among the total violations. The most frequent violated in antibiotics were ampicillin and tylosin, followed by sulfonamides, penicillins etc. In case of other drugs except antibiotics, vitamin A and glucocorticoids (dexamethasone and prednisolone) were violated frequently. The overall trend of violation rates gradually decreased from 2009 to 2012, suggesting that quality of veterinary medicines has improved according to Korea Veterinary Good Manufacturing Practice (KVGMP) system settled down in 1988.