The effects of intake system on turbulent kinetic energy for the in-cylinder flow of a four-valve SI engine were studied. For this study, the same head, cylinder, and the piston were used to examine turbulence characteristics in two different intake systems. In-cylinder flow measurements were conducted using three dimensional LDV system. The measurement method, which simultaneously collects 3-D velocity data, allowed a evaluation of turbulent kinetic energy inside a cylinder. High levels of turbulent kinetic energy were found in regions of high shear flow, attributed to the collisions of intake flows. These specific results support the more general conclusion that the slightly offset direction of the intake system produced higher in-cylinder velocities on the +x-axis side of the cylinder which caused some asymmetric flow patterns about the z-axis. Higher levels of turbulent kinetic energy prevailed in zones of mean velocity collisions and regions where significant directional changes in the mean velocity patterns occurred.