PURPOSES: This study aims to contribute to a better road environment, which can result in accident reduction from two-wheeled vehicles, by analyzing factors affecting the two-wheeled vehicles’ accident severities in Incheon Metropolitan City. METHODS: In this study, the two-wheeled vehicles’ accident severity was classified into four categories (fatal injury, serious injury, minor injury, and injury report) as a dependent variable, and 97 independent variables out of 14 categories were considered to construct an ordered probit model. To determine the factors affecting accident severity, the statistical package LIMDEP was used. RESULTS: Among the variables used in the analysis, variables related to accident occurrence date (first quarter), region (8-district), accident type (passing the edge of the road of the vehicle for a pedestrian accident, fixed object collision, and overturn of vehicle-only accident), violation type (unobtained safety distance, failure to perform safe driving, violation of intersection driving, and violation of others), the type of road (at the intersection, near the intersection, at the crosswalk, near the crosswalk, etc.), gender of assailant (male), vehicle of victim (pedestrian and motorcycle), and age of victim (under 20) were found to have a statistically significant effect on the severity of the accident. CONCLUSIONS: The variables related to accident type (fixed object collision and overturn of vehicle-only accident), gender of assailant (male), and vehicle of victim (pedestrian and motorcycle) have turned out increasing the accident severity. In addition, accident occurrence for two-wheeled vehicles is more diverse and vulnerable to damage than automobile accidents. Therefore, it is time to recognize the seriousness of two-wheeled vehicle accidents and to improve the environment and systems for safe driving.