Sex preselection has always generated great interest among livestock producers due to an increase in the profitability of the cattle industry through the production of offspring with desired sex, such as females for dairy or males for meat production. Among the prevalent sorting methods, the embryo developmental potential is still very low as expected, and there is distinguished evidence that sex sorting has a negative effect on sperm quality with an altered pattern of sperm motility, ultimately reducing lifespan. The consequence is a very low embryo development rate using sex-sorted semen, and its negative impact influences the progress of the dairy industry. Here, we established a new approach with reduced stress by using WholeMom® and observed no significant differences (P < 0.05) in early cleaving embryos between sorted X sperm and the control group, although there was a remarkable significant difference in embryos of the Y sperm group, 81.82 ± 2.71% vs. 87.44 ± 3.02% vs. 54.21 ± 2.21%, respectively. The percentage of embryos that developed into blastocysts (Day 7) was also significantly (P < 0.05) higher in the control and X Sperm group compared to the Y sperm group, 35.53 ± 1.92% and 29.76 ± 2.38% vs. 21.90 ± 1.54%. Moreover, B-SRY F2 and B-SRY R2 gene expression data exhibited 81.03% accuracy for the female embryos and 72.54% for the male embryos produced in vitro. And also the field trials for the heifer production using WholeMom by Artificial Insemination technique demonstrated 76% female and 24% male in vivo. In conclusion, the combination of pre-selected sex semen and OPU derived elite cattle embryo production is highly recommended to apply to the mass production in the dairy industry with rapid genetic up-gradation.