Leafbeetles of subfamily Zeugophorinae in Korea are revised. Z. annulata and Z. bicolor are known to the Korean peninsula until now. This subfamily is classified into only one genus, Zeugophora Kunze. Species of the genus are revised. Among them, Zeugophora (Pedrillia) trisignata sp. nov. is described as new to science. A key, external photos, geographical data and host plants to the three species of the genus, included description of new species, are provided.