The purpose of this study is to determine the possibility of using Crataegi fructus as natural health food source. The research was conducted to determine the biofunctional activities of Crataegi fructus extract. Methanolic extract from Crataegi fructus was partitioned by using organic solvents, including n-hexane, ethyl acetate, n butanol, and water. Ethyl acetate soluble fraction was shown to have the strongest antioxidant activity (RC₅₀=4.39 ㎍/㎖) among the fractions. In the antimicrobial activity assays, ethyl acetate soluble fraction was effective in bacterial inhibition, against the cases of Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in 125 ㎍/㎖. In the anticomplementary activity assays, water soluble fraction was the most effective exhibiting 18.4% inhibitory activity.