We present optical and near-infrared imaging and long-slit spectroscopy for the blue compact dwarf galaxy (BCD) Mrk 49 in the Virgo Cluster. The surface brightness distribution analysis shows that Mrk 49 consists of an off-centered blue bright compact core of r = 10′′ and a red faint outer exponential envelope. The Hα image and color difference suggest that these two components have different stellar populations: a high surface brightness population of massive young stars and an underlying low surface brightness population of older stars. The redder near-infrared colors of the inner most region suggest that the near-infrared flux of Mrk 49 originates from evolved massive stars associated with the current star-forming activity. The total apparent magnitude is BT = 14.32 mag and the mean effective surface brightness is μeff (B) = 21.56 mag arcsec−2. Long-slit spectroscopy shows that Mrk 49 rotates apparently as a solid body within r = 10′′ in a plane at position angle 55 degrees with an amplitude of about 20 km sec−1. The measured radial velocity of Mrk 49 was derived as 1,535 km sec−1; and the total mass of stars and gases is in the range of 3 to 6 × 109 M⊙. The mass-to-light ratios for the central region of Mrk 49 in I and B band are estimated 1.0 and 0.5, respectively. The upper limit of the dark matter to visible matter ratio seems to be < 5. The oxygen abundance is 12 + log(O/H) = 8.21 ± 0.1 which is about one quarter of the solar value while the relative helium abundance appears to be similar to that of the sun.