This study was carried out to evaluate the effect of applying levels of Bestamin, animal amino acid, on growth and quality of hot pepper and physicochemical properties of soil. Treatment was given with 200, 400, 800, 1600㎏ per 10a to control of Bestamin, difference of physicochemical properties was lower than Bestamin, and P₂O₅ seems to be reduced, also K⁺, Ca⁺⁺ were obviously low compared to the control. The content of NO₃-N was low compared to control at the Bestamin treated plot. Plant height was longest at 800 treatment and main stem length, main stem weigh and the number of leaves were significantly different with 400 and 800 treatment. No difference was found among the fruit weight, length, diameter in first harvest, but there was significantly different at control of 2nd, 3rd harvest and more increased than 400 and 800 treatment. Fruit weight per plant was the heaviest at 400 and 800 treatment, and the number of fruit was no difference at red pepper but increased with 400 and 800 at green pepper, and yield per l0a was significantly increased to 4503.6㎏ and 4582.5㎏, respectively. Nitrogen in mesophyll accumulation content was obviously reduced at Bestamin treatment compared to control, and amino acid was reduced with control.