In order to renovate spirodiclofen contaminated soil through biodegradation, a high-effective spirodiclofen degrading bacterium was isolated from corn cultivated soil in Weifang of Shandong, China using enrichment culture and HPLC method. The strain identification was studied, the best cultivated conditions and pesticides degrading substrate range of the strain was also determined. The spirodiclofen degradation rate of the screened strain WF14-6 was 76.42% after 120 h cultivation, based on morphology and 16S rRNA gene sequence analysis, the strain was identified as Enterobacter sp. The best cultivated temperature and pH of the strain were 30℃and 7.0. After 72 h cultivation, the degradation rate of strain to imidacloprid and acifluorfene was 31.35~41.06%, the degradation rate to bupirimate、diafenthiuron and dimethomorph was 20.81~23.90%.