A 12-year-old, spayed female, Pomeranian dog was referred for limbs stiffness and circling movement. Computed tomography(CT) and magnetic resonance imaging(MRI) revealed a contrast-enhanced space-occupying lesion suggestive of meningioma in the olfactory groove to the right frontal lobe. For a successful and accurate surgery, 3D printing technique for preoperative planning and 3D guide was applied. Surgical excision of the meningioma was successfully performed. The postoperative complications were managed through medical treatment and patient care without the revision surgery.

A meningioma is the second most common primary intracranial tumor of the central nervous system. One critical obstacle in meningioma research and preclinical development of novel therapeutic agents is a relative lack of suitable preclinical in vitro and in vivo model systems. In the current study, we assessed the proliferative characteristics of patient derived five primary meningioma cancer cell lines (WHO grade I and II) from brain tumor lesions. All of the meningioma cell lines showed homogenous expression of meningioma marker, VIMENTIN. The GTG-banding analysis determined the existence of different patterns of chromosomal abnormalities in representative cancer cell lines. The almost of the meningioma cell lines showed homogeneously spindle shaped cells, except for M160425 which have two prominent cell morphologies, spindle and round. Population-doubling (PD) was measured for every passage. The M160425 cell line had significantly longer PD time (39.8 ± 0.9 h, P<0.05) than the other meningioma cell lines. Consistent with the PD time, we confirmed that mRNA expression of Ki67, the conventional proliferation marker, was significantly lower in M160425 cell line compared to the other cell lines. The correlation between the PD time and the abundance of Ki67 in the meningioma derived cell lines was negative, indicating higher Ki67 abundance and a shorter PD time. The patient derived meningioma cancer cell lines established in this study might contribute to understanding cancer biology and help the success in the clinic by explaining the slightly different clinical behavior among the patients.

Meningiomas are tumors that arise from the leptomeninges. They are generally benign tumors, and only rarely do they undergo malignant transformation. Meningiomas account for approximately 15% of all brain tumors. Meningiomas grow very slowly, often over many years. Because these tumors grow so slowly, they can sometimes become extremely large before being detected. This was especially true in past eras, before advanced imaging modalities were available (i.e. CT scans and MRI scans). Today, with our multitude of MRI and CT scan machines, meningiomas are most often detected when they are relatively small. MRI scanning is currently the best diagnostic tool for detecting and defining meningiomas.

본 연구에서는 뇌수막종이 늦게 조영증강 된다는 점을 착안하여 뇌수막종을 최대 조영 증강시킨 검사를 시 작하였다, 검사 후에 두개내의 수막종과 혈관과의 관계를 알아보고 전산화 단층 촬영 조영술의 3차원 CT 영상 기법(이하, 3차원 CT 영상 기법)과 고식적 혈관 촬영 조영술의 영상을 비교하였다. 연구대상은 3차원 CT 영상 기법과 고식적 혈관 촬영 조영술을 병행 시행하였던 6명의 환자를 대상으로 하였으며. 그 중 추체접형골동사대 부 5예, 방시상동에 생긴 1예였다. 검사방법으로는 조영제를 초당 3/120 ml를 주입하여, GE Medical System Program(smart prep)을 이용하여 CT Number 값이 100에 도달한 후, 검사를 시작하였다. 스캔파라미터는 조영제 가 모두 주입된 직후에 1.25 mm / 3.75 mm, HQ-Mode로 scan한 후 1 mm interval로 재구성하였다. 검사한 영상을 3D-Med Software Program(Rapidia)을 이용하여 3차원 CT 영상 기법으로 영상을 재구성한 다음 고식적 혈관 촬영 조영술과 비교하였다. 결과를 살펴보면 뇌수막종의 최대조영 시간은 조영제을 주입한 후 약120-180초에 혈관과 종양과의 관계를 가장 잘 구별할 수 있었다. 3차원 CT 영상 기법으로 재구성한 6예는 모두 종양과 혈관과의 관계를 잘 구별할 수 있었다. 또한 종양과 동반된 동맥류도 1예에서 잘 보여주었다. 이를 종합하여 보면 두 개 내 뇌수막종의 환 자에서 조영제를 주입하여 3차원 CT 영상을 시행하였던 영상은 종양과 혈관과의 관계를 입체적으로 구분할 수 있어 수술에 많은 도움이 되었다.