The heat transfer characteristics of double-pipe spiral heat exchanger using aluminum oxide nano-fluid were investigated by three different sizes of curvature size, experimentally. Five concentration of nano-fluid as working fluid were made and tested to analyze the heat transfer characteristics. As results, the heat transfer performance was improved at 0.25% of nano-fluid due to high thermal conductivity, however, as the concentration of nanofluid increased (~2.0%), the heat transfer performance deteriorated due to the increase in thermal resistance caused by the sedimentation of particles in the flow path. In addition, the nano-fluid has a higher pressure drop than water due to its high density and viscosity. The optimal range for heat transfer enhancement of nano-fluid was found to be less than 4.0 LPM in flow rate and 0.25% of nano-fluid concentration in this study.