The study examined qualities including chlorophyll, total phenol, total flavonoid content and antioxidative activity in 7-year-old mistletoe that was artificially cultivated with Japanese apricots as the host. The chlorophyll a content of the artificially cultivated Japanese apricot mistletoe leaves was 7.67 mg/g (old leaves) and 3.17 mg/g (new leaves), while the chlorophyll b content was 0.80 ~ 3.53 mg/g and 1.31 ~ 3.56 mg/g. The carotenoid content of the Japanese apricot mistletoe leaves were in the range of 0.29 ~ 2.48 mg/g, and the old leaves (1.85 mg/g) had a carotenoid content 2.1 times higher than the new leaves (0.89 mg/g). The total polyphenol content of the leaves and nodes of the 7-year-old mistletoe was 60.22 mg/100 g TAE for the leaves and 35.50 mg/100 g TAE for the nodes. Among the host trees, polyphenol content was highest (81.44 mg/100 g TAE) in the old mistletoe leaves of the Quercus mongolica, while the total flavonoid content of the Japanese apricot mistletoe was higher in the new leaves (47.89 mg/100 g RUE) than the old leaves (42.19 mg/100 g RUE). The DPPH radical scavenging ability of the Japanese apricot mistletoe scored a low 22.4 ~ 28.5% in the leaves, but was relatively high in the nodes at 52.4 ~ 80.1%. In terms of 1mg/mL concentration, the new leaves (69.1%) and old leaves (82.3%) of the Quercus monglica mistletoe displayed the highest inhibition rate. Chestnut mistletoe displayed an inhibition rate of 21.9 ~ 31.9% for the leaves, and 35.0 ~ 63.8% for the nodes. These results indicate that Japanese apricot mistletoe would be substitute oak tree mistletoe as a source of bio-active property for functional food and medicine.
This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of 8.57 μg/mg and 3.83 μg/mg, respectively while the highest carotenoid content was found in MJ ml/L treatment with 1.62 μg/mg. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).