This study describes a hydrogen embrittlement evaluation of the subsurface zone in 590DP steel by micro-Vickers hardness measurement. The 590DP steel was designed to use in high-strength thin steel sheets as automotive materials. The test specimens were fabricated to 5 series varying the chemical composition through the process of casting and rolling. Electrochemical hydrogen charging was conducted on each specimen with varying current densities and charging times. The relationship between the embrittlement and hydrogen charging conditions was established by investigating the metallography. The micro-Vickers hardness was measured to evaluate the hydrogen embrittlement of the subsurface zone in addition to the microscopic investigation. The micro-Vickers hardness increased with the charging time at the surface. However, the changing ratio and maximum variation of hardness with depth were nearly the same value for each test specimen under the current density of 150 mA/cm2 and charging time of 50 hours. Consequently, it appears that hydrogen embrittlement in 590DP steel can be evaluated by micro-Vickers hardness measurement.