This study aimed to find out the effect of freeze-drying leek against cholesterol feeding rats on the cholesterol and lipid in serum, hemolysis in erythrocyte and blood pressure in rats tall. In this experiment, male rats of Wistar strain were used. The rats were divided into 3 groups in 8 weeks : basal diet, 1% cholesterol and 1% cholesterol and 1% leek. The followings are the results of this experiment. 1. In vitro, leek-ext, reduced the hemolysis. 2. Leek reduced VLDL, LDL/HDL-cholesterol in serum. 3. Leek reduced the blood pressure in rat's tail. 4. Leek reduced the fatty change in liver caused by cholesterol treatment. This experiment showed that leek-addition group had portective effect against cholesterol fed and decresed VLDL, LDL/HDL-cholesterol in serum, Leek alleviated hemolysis in erythrocyte, blood pressure in rat's tail and fatty change in liver. Therefore, this experiment concluded that leek has defensive power against cholesterol.