This study attempts to examine the existence of foreign language anxiety in e-Learning situations, and then identify relationships between the anxiety and learner background factors. A total of 162 EFL learners participated in the survey. Their anxiety was measured by the e-Foreign Language Classroom Anxiety Scale (e-FLCAS). The results suggest that foreign language learners do indeed experience anxiety in e-Learning classrooms. A majority of the participants acknowledged having experienced specific kinds of anxiety in cyber classes: worry over e-Learning education, online test anxiety, learning style conflicts, and apprehension of native teachers. In addition, e-Learning anxiety was found to be significantly related to three background factors (gender, academic backgrounds, and English proficiency). The results also provide a potential explanation of anxiety-producing contexts in cyber classrooms, the sources of anxiety, and coping strategies that students use to lower their anxiety.