한국약용작물학회지 Vol. 26 No. 4 (p.308-316)

|연구논문|
찔레꽃 에탄올추출물의 생리활성과 화장품 방부효과

Biological Activity and Cosmetic Preservative Effects of Rosa multiflora Ethanol Extracts
키워드 :
Rosa multiflora,Antimicrobial Activity,Antioxidative Activity,Cosmetic Preservative Effect

목차

ABSTRACTS
서 언
재료 및 방법
  1. 재료
  2. 찔레꽃 에탄올 추출물 제조
  3. DPPH 라디칼 소거 활성 측정
  4. 총 polyphenol 함량 측정
  5. 총 flavonoid 함량 측정
  6. Phenolic compounds에 대한 high performance liquidchromatography (HPLC) 분석
  7. Paper disk를 이용한 항균활성 측정
  8. 찔레꽃 에탄올 추출물 첨가에 따른 방부효과 (challengetest)
  9. 통계학적 분석
결과 및 고찰
  1. DPPH 라디칼 소거활성
  2. 총 polyphenol 및 flavonoid 함량
  3. 항균활성
  4. 화장품 방부효과
감사의 글
REFERENCES

초록

Background: The Rosa multiflora, a well-known plant belonging to Rosacea, is widely used in orthodox medicine in worldwide. However, its biological activity and cosmetic preservative efficacy have not yet been studied. Thus, this species is yet to be defined as a functional cosmetic material. Accordingly, an investigation of the above mentioned atrributes was performed on a 50% ethanol extract of Rosa multiflora.
Methods and Results: The antioxidant activity was assessed through free radical scavenging assays with 2,2-diphenyl-1-picrylhydrazyl (DPPH). Additionally, the contents of total phenols and flavonoids were analyzed. The phenolic compounds were detected using HPLC. The antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans was assessed using the disc diffusion assay. The preservative effect (challenge test) on a formulation of soothing gel was performed for 28days. The DPPH radical scavenging ability, denoted by the SC50 (half maximal inhibitory concentration for DPPH radical scavenging) value was found to be 131.63 ㎍/㎖. The content of total polyphenol and flavonoid content were 202 ㎎/g and 86.77 ㎎/g, respectively. In additon, astragalin and gallic acid were identified in the extract. The antimicrobial activity of the extract against S. aureus and E. coli was observed to be 5 - 0.5%, and no significant activity was noted against C. albicans. The ethanol extracts (5% and 10%) met the preservation standards of the Cosmetics, Toiletry, and Fragrance Association (CTFA).
Conclusions: Thus the ethanol extract of R. multiflora can be used in cosmetics as a natural preservative and antioxidant.