Fifty-nine Korean soybean (Glycine max L. Merr.) landrace accessions were tested for genotype fingerprinting, differentiation and association between morphological traits and SSR profile. Using 8 SSR loci, 59 varieties were divided into 55 groups, and only 4 pairs of varieties were not uniquely identified. The resolving power of SSR for soybean genotyping was much higher than that of the morphological traits that were studied. Identification efficiency also differed among SSR loci. Those loci with higher numbers of alleles distinguished varieties more effectively. Genetic differentiation values of the soybean landraces varied from 0.57 to 0.82 with a mean of 0.68. The number of alleles detected by the 8 loci ranged from 3 to 8. and the effective number of alleles ranged from 2.3 to 5.1. In a study of the association of SSR alleles with morphological traits, some alleles seemed to be related with some specific morphological traits. Comparison of two kinds of dendrograms which were derived from SSR markers and quantitative traits indicated that the dendrograms were not consistent. Considering the correlation between single SSR locus and qualitative traits governed by major genes, the data suggest that alleles of microsatellite loci be more closely related to some traits determined by major genes than those determined by minor genes.

An isolate of barley yellow mosaic virus(BaYMV-HN) obtained from Haenam, Korea was compared with two BaYMV strains. BaYMV-Ⅱ-1 from Japan and BaYMV-G from Germany. The sequence of the 3'-terminal 3817nucleotides[excluding the poly (A) tail] of RNA 1 of BaYMV-HN was determined to start within a long open reading frame coding for a part of the NIa-VPg polymerase(26 amino acids). NIa-Pro polymerase (343 amino acids), NIb polymerase(528 amino acids) and the entire capsid protein(297 amino acids), which is followed by a noncoding region(NCR) of 235 nucelotides. In the partial ORFs, BaYMV-HN shows higher sequence homology with BaYMV-Ⅱ-1(99.5%) than BaYMV-G(92.7%). The 3' non-coding regions of BaYMV-HN(235nt) shows higher nucleotide sequence homology with BaYMV-G(235nt)(99.6%) than BaYMV-Ⅱ-1(231nt)(97.0%). The 3' NIa-Pro protein sequence of BaYMV-HN shows higher amino acid sequence homology with BaYMV-Ⅱ-1(95.0%) than BaYMV-G(93.6%), but, NIb protein sequence of BaYMV-HN shows same all amino acid sequence. The capsid protein sequence of BaYMV-HN(297aa) shows same with BaYMV-Ⅱ-1, and shows higher nucleotide sequence homology with BaYMV-UK (from United Kingdom)(97.3%) than BaYMV-G(96.9%) and G2(96.9%). Difference of capsid protein amino acid were 0-9 between the Japan, United Kingdom and Germany and were 2-6 between all Korean isolates. Many of the amino acid differences are located in the N-terminal regions of the capsid proteins from 1 to 74 amino acid positions.

The effects of basal media, sucrose and phytohormone concentrations, and gelling agent combinations on in vitro frond proliferation of Lemna gibba G3 and 24 additional Lemna gibba strains were examined. Frond proliferation was equivalent on Schenk and Hidebrand. Murashige and Skoog. Nitsch and Nitsch, and Gamborg's B5 media and poor on murashige and Skoog medium in the absence of benzyladenine. With the addition of benzyladenine, Schenk and Hildebrand and Gamborg's B5 Were superior and equivalent. The addition of benzuyladenine increased equally frond proliferation at either 1 or 10μM, however at 10μM fronds were severely curled or fused. Benzyladenine and thidiazuron suppressed root growth but kinetin was found to greatly enhance root growth. Gibberellic acid inhibited frond proliferation. Frond proliferation was significantly different on the four sucrose concentrations of 0, 1, 3, and 5% Among them, 3% sucrose was found to be superior. The reduced frond size observed in cultures grown on 8% sucrose could be explained by showing medium osmotic potential in excess of frond water potential. Gell agents also varied significantly in their ability to promote frond proliferation with 0.25% Gelrite or a mixture of 0.15% Gelrite and 0.4% agar. Proliferation of 25 Lemna gibba strains on medium neat optimal for Lemna gibba G3 showed a six-fold variation across strains with Lemna gobba G3 placing in the top 5 fastest proliferating strains.

These studies had performed a plans for preservation and distribution of Ilex cormuta community Mt. Keumsung(35˚05' N, 126˚04' E) in Naju, Chonnam from Arpil, 1997 to October by Braun-Blanquet' method(1964). According to the vegetation table of community, the plant communities of the investigated sites were classified Ilex corunta-Trachelospermum asiaticum var. intermedium commnity. Now there still remains the natural environment to be developed in coast district around the riverside distributing Ilex cornuta Lindl., so it has scientifically high value to predict change of the ecosystem and preserve the community distribution. It needs plan for preservation high value to predict change of the ecosystem and preserve the community distribution. It needs plan for preservation

Aloe in vitro culture was attempted to induce callus and regeneration ability from different explant sources onto MS medium with 0.5mg/l NAA plus 1.0mg/l BA. Anthers that no developed any callus and plant regeneration, while only four out of 274 filament explants induced calli at cut edge without regenerated plants. Twenty ovary explants regenerated four direct plantlets without via callus from the base of epidermal tissues. Regenerated plants on the root tip gave 2n=14 of chromosome numbers.

Soybean is an important crop because its seed has very high protein relative to others. The quality of soy protein is limited by the concentration of the sulfur-containing amino acids in the amino acid profile. Among the supply of various forms of 0.4mM sulfur as S nutrition during seed fill. only 0.4mM L-methionine can inhibit β-subunit synthesis completely and produce the highest glycinin-containing seeds. Compared to 0.4mM sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration. Supply of 0.2mM cystine (0.4mM S) did not affect the accumulative pattern of seed storage protein (SSP) subunits. In the supply of L-methionine, 0.2mM treatment showed higher glycinin in seeds but 0.05mM resulted in lower glycinin than tile sulfate control. The relative abundance of α`-subunit was not altered by any N or S nutrition. Under 5mM nitrogen, protein concentration was increased about 3-5% by substituting ammonia for nitrate during seed fill independent of nutrition. The increase resulted in the only increase of 7S protein, mainly β-subunit. Our data suggest that the regulatory system of SSP genes responds to the balance between N and S assimilates supplied from mother plant. and controls the di fferential synthesis of their subunits for the maximum protein accumulation in developing soybean seed.

Wood and bark structure of Laurus, which is considered as an important spice resources was characterized by several transitional and advanced features. Annual rings are distinct: vessel elements are moderately short and narrow, both simple and sealariform perforation plates. alternate intervascular pitting; irregularly septate fiber are present; parenchyma strands are scanty paratracheal: rays are both homogeneous and heterogeneous with multiseriate: phloem rays are dilated. Comparisons with other genera of Lauraceae suggest that Laurus is transitional and advanced state in evolutionary trends in the family.

Many higher fungi of ascomycetes were collected at Mt.Odae, Mt.Moak, Mt.Jiri, Mt.Hanla, Mt.Manduck and Mt. Yonsuk form 1996 to 1997. They were identified and according to the result, the genera of Lachnellula, Encoelia and Hymenoscyphus and the species Peziza Praetervisa, Lachnellula pseudofarinacea, Dasyschyphus acutipilus, Encoelia Furfuracea, Hymenoscyphus equisetinus, Hypocera pulvinata, Nectria coryli and Nectria ellisii are newly to Korea. Korean common names of them were designed by author.

Many higher fungi were collected at Mt.Jiri, Mt.Moak, Mt.Sunun, Mt.Obong, Mt. Yonsuk, and Samrey-up from 1995 to 1997. They were identified and according to the results. Genus Myxomphalia and species of Lentinellus ursinus, Marasmius torquescens, Omphalina griseopallida, Myxomphalia Maura, Amanita perpasta, Pasthyrella bipellis, Conocybe aurea and Agrocybe farinacea were newly to Korea.