The development of drugs from natural plant sources is at growing interest due to the limitations of chemical drugs in terms of side effects and cost-effective factors of natural medicines. Among the various components contained in natural plant materials, flavonoids are of increasing interest because of their extended biological benefits. Flavonoids are classified into various types according to their structure and possess different activities depending on the structure. In this study, the flavonoids contained in Artemisia, native to Korea were examined and reviewed. HPLC chromatograms of three Artemisia species (Artemisia annua L., Artemisia iwayomogi and Artemisia argyi H.) were examined from published sources and their component analysis by MS data were summarized. The various flavonoids of Artemisia were classified into 12 types according to the main structure, and 10 flavonoids based on various activities were examined. The 10 flavonoids were identified as quercetin, kaempferol, rhamnetin, diosmetin, luteolin, methoxyflavone, catechin, apigenin, malvidin and genkwanin with extensive reported studies till date. The ten flavonoids examined have been reported to be effective in preventing and treating various diseases and exhibit activities such as anti-cancer, anti-inflammatory, antioxidant, antiviral, anti-obesity, anti-diabetic and anti-Alzheimer. The collective results from the reported studies suggest that the three types of Korean native Artemisia, contains various flavonoids with beneficial activities and may have therapeutic effects against diseases.

The purpose of this study was to determine the effect of waterlogging duration on the growth characteristics and productivity of forage corn at different growth stages under paddy field conditions. Treatments consisted of waterlogging at two growth stages (V7 or V14) for four waterlogging durations (no waterlogging, 48 hours, 72 hours, and 96 hours, respectively). The V14 growth stage was more vulnerable to waterlogging than the V7 stage. Among the waterlogging durations, the lodging score increased at 48 hours. The stem height of forage corn decreased with the increase in waterlogging duration at the different growth stages (V7 and V14). Increase in waterlogging duration reduced the stem dry matter yield, ear dry matter yield, and total dry matter yield at both growing stages (V7 and V14). The waterlogging treatments at the V14 stage affected ear dry matter yield more than those at the V7 growing stage. Thus, the management of forage corn under paddy field conditions must be strengthened during early (V7) and grain fill stages (V14). When waterlogging occurs, surface and subsurface drainage should be implemented within 48 hours to control (no waterlogging) the groundwater level and, thus, minimize economic losses due to forage corn damage.

강원지역에 발생하는 돌발해충은 미국선녀벌레, 갈색날개매미충, 꽃매미 등이 있다. 이러한 돌발해충의 월동은 동계최저기온에 의해 가장 큰 영향을 받게 된다. 따라서 꽃매미는 2012년부터 갈색날개매미충은 2016년부터 월동난괴 를 채집하여 동계최저기온에 따른 부화율을 측정하여, 동계최저기온과 돌발해충의 부화율 관계에 대한 조사를 실시하였다. 동계최저기온이 –11℃이하인 날이 35일 이상 지속된 2012년과 2017년 겨울은 꽃매미의 부화율이 9.2%, 7.2%로 낮게 나타난 반면, -11℃이하인 날이 16일로 비교적 낮은 경과일수를 보인 2013년 겨울에는 75.5%의 부화율을 보였다. 갈색날개매미충 역시 –11℃이하인 날이 35일 이상이었던 2017년 겨울에 43.1%의 낮은 부화율을 보였지만 20일로 적은 경과일수를 보인 2016년 겨울에는 89.4%로 약 2배정도 높은 부화율을 확인할 수 있었다. 이는 일정수준 이상 동계최저기온을 경과한 돌발해충의 월동난은 성공적인 월동을 하지 못한다는 것을 의미한다.

에메리개미는 여왕개미와 수개미가 유전적으로 복제되어 번식한다고 알려져 있으며, 여왕개미의 날개형태가 장시형과 단시형으로 나타난다. 장시형은 정상적인 날개형태이고, 이보다 짧은 날개형태는 단시형이라고 한다. 장시형과 단시형 모두 한 종으로 취급되지만, 두 가지 점에서 종 지위에 대한 조사가 필요하다. 첫째, 자연 상태에서는 두 날개형이 함께 발견되지 않고, 둘째, 날개형이 육안으로 뚜렷하게 구분된다. 또한 복제되어 번식한 여왕개미가 단수체인지 배수체인지 조사가 필요하다. 따라서 우리는 본 연구에서 에메리개미 유전체 크기를 추정하여 두 날개형은 동종이며, 여왕개미는 배수체임을 확인하였다.

연 10회 이상 발생하는 온실가루이는 토마토 재배 시 6월 하순 기온이 상승하면서부터 발생량이 급격히 증가하고 약제에 대한 저항성이 강해 많은 농가에서 방제에 많은 어려움을 겪고 있다. 이러한 온실가루이의 효과적인 방제를 위해 최적교호살포 약제조합을 선발하는 실험을 실시하였다. 작용기작에 따라 약제는 각각 신경전달기능을 억제하고, 키틴생합성을 저해하며, 지질생합성을 저해하는 작용기작을 가진 약제로 선정하였다. 각 처리구별로 약제 살포순서를 다르게 하여 교호살포한 결과 모든 처리구에서 3회 약제처리 후 성충밀도가 처리전 밀도보다 유의한 차로 줄어들었다. 약충에 있어서는 약제처리전 모든 처리구에서 약충 발생이 낮아 정확한 밀도억제효과를 확인하기 어려웠지만 최종조사에서는 신경전달저해, 탈피저해, 지질생합성저해 약제 순으로 처리한 처리구의 밀도가 무처리구와 비교하였을 때 일정수준 억제되는것을 확인하였다

2018년 강원도 춘천의 경우 동계최저기온(12월 상순~2월 중순)은 전년대비 약 0.2~9℃낮은 것으로 나타났다. 이에따라 돌발해충의 월동난 부화율이 감소하는 추세를 보였으며 실제로 갈색날개매미충은 전년대비 약52%, 꽃매미는 약67% 부화율이 낮아졌다. 또한 최근 강원도에서 발생면적이 증가하고 있는 미국선녀벌레는 5월 하순에 부화하여 7월 상순에 인삼포장에서 주당 최대 12마리(중산간지)의 약충발생밀도를 보였고, 약제방제가 이루어지지않은 아까시나무에서는 7월상순 가지당 312마리(산간지)의 최고발생밀도를 보였다. 갈색날개매미충의 경우 복숭아과원에서의 발생은 많지 않았지만 과원주변 수목에서는 7월상순 가지당 최고 73마리(산간지)의 약충이 발생하였다. 꽃매미는 5월 하순 부화가 시작된 후 포도과원에서 주당 최고 12마리(산간지)의 약충이 발생하였지만 약제방제를 통해 시간이 지남에 따라 밀도가 점차 줄어드는 것을 확인하였고, 볼록총채벌레는 8월 상순부터 발생이 시작되어 9월 상순 트랩당 4.8마리(평야지)의 발생밀도를 보였다.

The objective of this study was to investigate the dynamic neural mobilization program on the changes in muscle activity and nerve conduction velocity (NVC) in stroke patients. The participants were sampled and randomly divided into experimental group I (n=12) who underwent arm neural mobilization and experimental group II (n=13) who underwent arm dynamic neural mobilization. As the pretest, peripheral NVC of the radial, median, and ulnar nerves were measured using the Viking Quest; the biceps brachii, brachioradialis, flexor carpi radialis, and extensor carpi radialis activities were measured with sEMG. Each intervention program consisted of 10 trials per set and three sets per session. The intervention programs were performed once daily for four weeks (four days/week). Posttest measurements were taken equally as the pretest measurements. Significant differences in peripheral NVC in all sections of the radial and median nerves and wristbelow elbow and below elbow-above elbow areas of the ulnar nerve, as well as in muscle activity of all muscles except the biceps brachii. These findings indicate that dynamic neural mobilization was effective in increasing peripheral NVC and altering the muscle activity.

최근 강원도에서 생산되기 시작하는 잎새버섯의 수확후배지를 활용하여 산업곤충의 한 종인 장수풍뎅이의 사육가 능성을 알아보고 장수풍뎅이 사육농가의 유충먹이 구입 시 경제적 비용절감 및 버섯재배농가의 부산물활용에 따른 신 소득을 창출하고자 시험을 추진하였다. 잎새버섯 배지는 참나무 85%와 밀기울 15%의 조성으로 이루어져 장수풍뎅이의 사육에는 무리가 없어 보였으며, 실제 유충 2령충을 4개월 사육한 결과 개체중이 21.6g으로 대조배지(참 나무발효톱밥) 개체중 20.2g보다 양호하였으며 폐사율도 낮게 나타났다. 유충의 가능한 사육밀도를 알아보기 위해 일반적으로 제시하는 밀도(75마리/60L)를 기준으로 0.5, 1.5배의 밀도를 사육한 결과 60L용기에 100마리 정도 사육이 가능하였다. 성충의 암수비율에 따른 산란시험에서는 암수비율 동일조건에서 산란수가 많았으며 잎새버섯 수확후배 지에서보다는 참나무 발효톱밥에서 산란이 양호하였다. 또한 산란효과를 높이기 위해 시판젤리에 여러 가지 첨가제를 넣어 시험한 결과 흑설탕을 첨가한 먹이조건에서 산란이 좀 더 양호한 것으로 나타났다.

The purpose of this study was to investigate the effect of traction and decompression therapies on the cervical muscle tone and disc height. The decompression group (n=16) received decompression therapy and Mckenzie exercises once a day and four times a week respectively, for three weeks. The traction group (n=15) also received traction therapy and Mckenzie exercises for the same period. Muscle tone was measured with a myotonometer, and the disk height was measured using magnetic resonance imaging (MRI), before the interventions. Three weeks later, we investigated the therapeutic effect by repeating these measurements. The difference in disk height between the two groups was not significant. There was a significant difference in the disk herniation index (p<.05). A significant difference was found only in the upper trapezius muscle after comparison of muscle tone and stiffness between the groups (p<.05). Findings from this study suggest that the decompression therapy is a more effective intervention for patients with cervical intervertebral disc herniation.

Vollenhovia emeryi (Formicidae: Myrmicinae) is divided into two morphs based on the wing length of the queen caste: the long-winged with a normal wing and the short-winged with the aberrant short wing. The phylogenetic analysis shows that the short-winged is derived from the long-winged. In Korea, only the long-winged morph is infected with Wolbachia while the short-winged is devoid of the bacterium suggesting that the short-winged evolved the resistance to the bacterial infection. Intriguingly, some Japanese short-winged colonies proved to still be infected with the bacterium. We hypothesized that the infected Japanese short-winged is the intermediate form in the process to complete cure. However, the data of the MLST and the measurement of Wolbachia density did not support our hypothesis. Our further experiment using microsatellite markers shows that the infected Japanese short-winged shows the similar genetic background to the long-winged. In this presentation we will discuss the potential resistance evolution in the Korean short-winged and future research direction at the genome level.

Nitrogen (N) fertilizer management is one of the important aspects of economic production of sorghums in sustainable agriculture. The aim of the study was to evaluate the effects of different N application rates and its split N application methods on productivity, growth characteristics, N accumulation, N use efficiency (NUE), and feed value of Sorghum × Sudangrass hybrids. Treatments consisted of five N application rates (0, 150, 200, 250, and 300 kg ha-1) and two split N application methods (40% in basal N, 30% at the growing stage, and 30% after the first harvest vs. 50% in basal N and 50% after the first harvest). Plant height, leaf width, and stem diameter were increased (p≤0.05) with increasing N fertility rates at each harvest. Chlorophyll content (expressed as SPAD values) was the highest at a rate of 300 kg N ha 1 (first harvest, 46.32; second harvest, 33.09). It was the lowest at zero N (first harvest, 21.56; second harvest, 18.5). Total N, N uptake, and NUE were increased with higher N rates. Split N application had little effect on total N, amount of N uptake, or NUE. Total dry matter yields were the highest (21,715 kg ha-1) at a rate of 300 kg N ha-1. It was the lowest (10,054 kg ha-1) at zero N. Our results suggest that more than 300 kg N ha-1 can improve dry matter yield to be above 116% compared to zero N, thus enhancing the agronomic characters of sorghums. However, no significant effect had been found for split N application. Further work is needed to determine the optimal N levels and the effect of split N application rates.

In every summer, cicadas emerge and become numerically and ecologically dominant in Korean penninsula. Especially, cicada emergence is affected by the environmental factors. In order to evaluate the effect of environmental factors in cicada species, we analyzed the temporal changes in cicada exuviae based on meteorological and non-meteorological factors such as artificial light intensity and habitat characters on urban park area surrounded by residential houses. Combined multivariate analyses with a cluster analysis and a principal component analysis (PCA) were conducted. Samples were classified into 3 different cluster based on differences of meteorological factors such as temperature and humidity. Moreover, Random Forest model (RF) showed a high predictability of daily mean temperature on species peak abundance. These results provide a strong evidence that meteorological factors have significant effects on cicada emergences. Regarding the non-meteorological factors, we found no relationship in cicada emergence.

Vollenhovia emeryi (Hymenoptera: Myrmicinae) is dimorphic in its wing morphology of alate females: the long-winged and the short-winged. In our previous study, we found that the long-winged is ancestral and the short-winged is derived. Intriguingly, the former is infected with the intracellular symbiotic Wolbachia bacterium and the derived is void of the bacterium indicating that the latter somehow evolved resistance to the bacterium. This may be one of few cases in which transition from susceptibility to the bacterium can be traceable via the divergence estimation. As a consequence, we inferred that the two morphs diverged approximately quarter million years ago; a remarkably recent event in evolutionary perspective. In this presentation, we will further discuss genetic orchestration in the host insect and future research directions.

The effects of seed soaking treatment with the solutions of plant growth regulators IAA, GA3 and BAP on seed germination and shoot and bulb growth of Allium victorialis var. platyphyllum (Korean wild garlic) were determined. A significant variation in the seed germination rate was recorded at all treatments for various soaking periods. Maximum seed germination was obtained when seeds were soaked in IAA or GA3 solution at 200 mg L-1. The MAP treated seeds started to germinate after 3 months. Among treatments, IAA was found to be most effective in improving seed germination, but further seedling growth was not correlated to the soaking time. Seed soaking in IAA or GA3 solution enhanced further growth of seedlings compared with water control treatment. Shoot and bulb growth was highest in GA3 treatments.