Amplified fragment length polymorphism (AFLP) is one of molecular marker technique based on DNA and is extremely useful in detection of high polymorphism between closely related genotypes like Korean wheat cultivars. Six Korean wheat cultivar specific marker sets have been developed from inter simple sequence repeat (ISSR) analysis and we can identify the 13 Koran wheat cultivars form other cultivars using six that (Son et al., 2013). We used four combinations of primer sets in our AFLP analysis for developing additional cultivar specific markers in Korean wheat. Twenty-one of the AFLP bands were isolated from ACG/M-CAC primer combination and 19 bands were isolated from E-AGC/M-CTG primer combination, respectively. We used forty bands to design sequence characterized amplified region (SCAR) primer pairs for Korean wheat cultivar identification. Only one of 40 amplified primer pairs, C2, were able to use for wheat cultivar identification. The DNA band of 215bp length was amplified by C2 primer pairs in ten cultivars, Eunpa, Olgeuru, Gobun, Saeol, Milsung, Sinmichal, Jokyung, Sugang, Goso, and Joah. Then C2 primer was applied to these primer sets as newly SCAR marker, six cultivars are identifying from other cultivars, additionally. Finally, to use the C2 and six primer sets, 19 Korean wheat cultivars are identified.

“Sooan”, a winter wheat(Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Keumkang/Eunpa//Keumkang” during 1997. “Sooan” was evaluated as “Iksan319” in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. “Sooan” is an awned, semi-dwarf and hard winter wheat, similar to “Keumkang” (check cultivar). The heading and maturing date of “Sooan” were similar to “Keumkang”. “Sooan” had similar test weight (817 g/L) and lower 1,000-grain weigh (43.7g) than “Keumkang” (817 g/L and 46.2g, respectively). “Sooan” showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the low ridge (0.2%) and rate of pre-harvest sprouting (0.2%) than “Keumkang” (9.2 and 25.8%, respectively). “Sooan” showed lower protein content (12.7%), SDS-sedimentation volume (42.0ml) and gluten content (10.5%) than “Keumkang” (13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (90.08) and lower redness (-1.11) and yellowness (8.80) in flour color than “Keumkang” (89.81, -1.15 and 9.46, respectively). “Sooan” showed higher lightness (81.34) of noodle dough sheet than “Keumkang” (80.28). “Sooan” exhibited lower hardness (3.84N) and similar springiness and cohesiveness of cooked noodles (0.91 and 0.65) compared to “Keumkang” (4.54N, 0.93, and 0.63, respectively). Average yield of “Sooan” in the regional adaptation yield trial test was 5.94 MT/ha in upland and 5.63 MT/ha in paddy field, which was 3% and 8% higher than those of “Keumkang” (579 MT/ha and 520 MT/ha, respectively).

“Joongmo2003”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “SW86054/Keumkang” during 1997. “Joongmo2003” was evaluated as “Iksan318” in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. Its heading date was April 24 in upland and April 21 in paddy filed conditions, which were two days earlier than those of the check cultivar Keumkang, respectively, and Maturing date was June 4 in upland and June 2 in paddy field, one or two days earlier than those of Keumkang, respectively. “Joongmo2003” showed resistance to winter hardiness and pre-harvest sprouting, which lower withering rate on the low ridge (0.3%) and rate of pre-harvest sprouting (18.5%) than “Keumkang” (9.2 and 25.8%, respectively).“Joongmo2003” showed similar protein content (13.2%), lower SDS-sedimentation volume (54.0ml) and higher gluten content (11.7%) than “Keumkang” (13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (89.85) of flour and noodle dough sheet than “Keumkang” (89.81 and 80.28, respectively). “Joongmo2003” exhibited similar hardness (3.84), springiness and cohesiveness of cooked noodles (0.92 and 0.63) compared to “Keumkang” (4.54N, 0.93, and 0.63, respectively). The average grain yield in the regional yield trial was 5.55MT/ha in upland and 4.85 MT/ha in paddy field, which were 4% and 7% lower than those of the check cultivar, Keumkang, respectively.

“Joongmo2004”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Bacanora88/Keumkang//Keumkang” during 1996. “Joongmo2004” was evaluated as “Iksan320” in advance yield trial test in 2006. It was tested in the regional yield trial test between 2007 and 2009. Its heading date was April 26 in upland and April 23 in paddy filed conditions, and Maturing date was June 5 in upland and June 4 in paddy field, which were similar to Keumkang, respectively. It showed 830 in number of spikes per m2, 35 of grain number per spike, 44.9g of 1,000 grain weight, and 806g of test weight. “Joongmo2004” showed moderate to Fusarium head blight (Scab) in test of specific character although “Keumkang” is susceptible to scab. “Joongmo2004” had higher flour yield (75.5%) than “Keumkang” (72.5% and 0.42%, respectively). “Joongmo2004” showed similar ash (0.42%), lower protein content (12.3%), SDS-sedimentation volume (43.0ml) and gluten content (10.3%) than “Keumkang” (0.42%, 13.1%, 57.8ml and 10.8%, respectively). It showed higher lightness (89.97), redness (-1.38) and yellowness (10.76) in flour color than “Keumkang” (89.61, -1.15 and 9.46, respectively). “Joongmo2004” exhibited lower hardness (3.50N), similar springiness and higher cohesiveness of cooked noodles (0.92 and 0.65) compared to “Keumkang” (4.54N, 0.93, and 0.63, respectively). The average grain yield in the regional yield trial was 5.17MT/ha in upland and 5.34 MT/ha in paddy field, which were lower 11% and higher 3% than those of the check cultivar, Keumkang, respectively.

“Joongmo2012”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Sinmichal/Keumkang” during 2006. “Joongmo2012” was evaluated as “Iksan357” in advance yield trial test in 2010. It was tested in the regional yield trial test between 2011 and 2013. Its heading date was May 2 in upland and May 1 in paddy filed conditions, and Maturing date was June 8 in upland and June 8 in paddy field, which were similar to Keumkang, respectively. It showed 628 in number of spikes per m2, 34 of grain number per spike, 47.6g of 1,000 grain weight, and 762g of test weight. “Joongmo2012” showed susceptible to Fusarium head blight (Scab) in test of specific character although “Keumkang” is susceptible to scab. “Joongmo2012” had lower flour yield (68.4%) than “Keumkang” (73.5%, respectively). “Joongmo2012” showed similar ash (0.40%), lower protein content (11.7%), SDSsedimentation volume (39.8ml) and gluten content (8.1%) than “Keumkang” (0.41%, 12.7%, 55.8ml and 9.2%, respectively). It showed higher lightness (92.70) in flour color than “Keumkang” (91.51, respectively). “Joongmo2012” exhibited lower hardness (3.19N), similar springiness and higher cohesiveness of cooked noodles (0.90 and 0.66) compared to “Keumkang” (4.21N, 0.90, and 0.62, respectively). The average grain yield in the regional yield trial was 5.48MT/ha in upland and 4.47 MT/ha in paddy field, which were higher 5% and similar than those of the check cultivar, Keumkang, respectively

Stem rust of wheat caused by Puccinia graminis f. sp. Tritici is historically one of the most important diseases of crops worldwide. The objective of this study was to screen the growth habits and yield of wheat accessions in Korea winter condition. We collected ninety stem rust resistant germplasms from USDA-Grain and planted for the characteristics of growth and yield in 2013. We measured the growth characteristics such as the plant type, leaf color, heading date, stem length, spike length, awn length, number of spikelet, number of tiller, and 1,000 kernel weight (g). Early maturity is most important wheat breeding target in double cropping system such as Korea. Heading dates of ninety cultivars ranged from May 17 to June 7. The Keumkang control cultivar headed on May 23. Heading dates of seventeen accessions had as early as Keumkang control (May 23). The numbers of semi-erect and intermediate type for growth habit were 45 and 33 respectively. The 1000 kernel weights were also measured. Mean 1000 kernel weights ranged from 16.7g (Webster) to 41.8g (123 A1C2). The average of stem length was 100.2cm and Keumkang was 58.4cm. These results showed that A4849-60P-7P-1P (Chile), MG 31492 (Ethiopia), 1338.F.6.C.3.B (Kenya), 1203 A1C2 (Kenya), and II-90-20/3-1R-1M- 3R (Mexico) were best lines for agronomic characteristic such as early heading date and grain yield

Characteristics of agronomy, especially good yield productivity is very important in wheat (Triticum aestivum L.) as human food crop. Development of elite cultivar having good traits is required for food stability. In 2013, a new wheat line called Iksan370 has been developed by the National Institute of Crop Science, RDA. Iksan370 has hard red winter wheat characteristics and better features like resistance to biotic, abiotic stress and higher yield ability than Keumkang. Heading and maturing date were delayed about ten days and period to flowering date was shorter three or four days than Keumkang. Culm and spike length of Iksan370 were 95cm and 15.3cm, averagely. This means that culm and spike length were longer than Keumkang (79cm and 7cm, respectively). It showed higher milling rate (75.5%), protein content (13.5%) and SDS-sedimentation volume (77.5ml) than Keumkang. Genotypes of six genes, GBSSA, B, D, PPO18, Pina and Pinb, were represented that were similar to Keumkang, respectively. Average yield of Iksan370 in the preliminary trial test was 6.61 tons/ha in upland. We expect that yield productivity and farm income were increased by Iksan370.

Fusarium head blight (FHB), primarily caused by Fusarium graminearum is a major disease problem on wheat and barley around the world. The objectives of this study were to evaluate for FHB resistance under greenhouse and filed condition in 2012 and 2013. We screened for Type I resistance in the field using spray inoculation and for Type II resistance in the greenhouse using single floret inoculation. Sumai 3 and Ning7840 were used the FHB resistant check. Five hundred and twelve lines were evaluated for resistance to initial infection (type I) and to spread of symptoms within spike (type II). The inoculum was F. graminearum (GZ3639) prepared at a concentration of 1 X 105 macroconidai/ml with Tween 20. The field screening plots were inoculated twice and mist-irrigation was applied to facilitate FHB development. FHB severity was assessed visually 21 days and determined as the percentage of symptomatic spikelets from the total of all spikelets observed in 20 spikes. Both in the greenhouse and field test, we could observe five categories of FHB severity: resistant (R: 0-20%), moderately resistant (MR: 21-40%), moderate (M: 41-60%), moderately susceptible (MS: 61-80%), and susceptible (S: 81-100%). The results showed that seventy seven lines showed the R and MR category on FHB severity. In addition, nine lines showed similar FHB severity compared to Sumai 3 (13.3%).

본 연구는 국내 밀 품종의 국수 색깔 및 이와 관련된 종실 및 밀가루 특성 평가를 통하여 국수 면대 색깔 향상을 위해 선발에 이용할 수 있는 방법을 개발하고, 특히 철함량이 국수면대 밝기에 미치는 영향을 평가하여 영양학적으로 우수한 철 고함유 계통 선발을 위한 간이검정법의 적용을 위해 수행하였다. 국내 밀 품종의 밀가루 밝기는 회분(r =-0.634, P<0.01), 단백질 함량(r = -0.635, P<0.01)및 총 폴리페놀함량(r = -0.493, P<0.05)과 부의 상관을 나타내었다. 면대 색깔은 밀가루 명도(r = 0.684, P<0.001)와 정의 상관을 보였고, 종실특성에서 리터중, 천립중, PPO 활성 및 총 폴리페놀 함량과는 부의 상관을 보였다. 또한 밀가루 특성에서 회분, 단백질 함량과 총 폴리페놀함량은 면대 밝기와 부의 상관을 보였고, 연차간 및 품종간 변이에 영향을 받는것으로 나타났다. 철은 밀가루 색 및 국수 면대 밝기와 부의상관을 나타냈고, 간이검정법으로 Potassium hexacyanoferrate(II) trihydrate(PHT) 처리를 한 결과 푸른색으로 염색되었다. 종실을 PHT로 염색하여 측정한 색도와 종실의 철함량은 상관을 보이지 않았으나, 종실을 분쇄한 통밀가루에서는 PHT로 염색된 통밀가루의 적색도(r = -0.665, P<0.001)와 황색도(r = -0.658, P<0.001)와 부의 상관을 나타내었다. 또한 PHT로 염색된 밀가루의 밝기는 종실의 철함량(r = -0.640, P<0.01)과 부의 상관을 나타내었다.

국내 밀 재배 환경에서 수발아 저항성 밀 품종 육성의 기초 자료를 제공하기 위하여 금강밀 등 31 품종을 이용하여 2년 동안 조사한 농업 형질과 종자 특성이 수발아율 및 GI에 미치 는 영향을 평가하였다. 농업형질은 출수기, 성숙기, 간장, 수 장, 망장, 경수와 1수립수를 조사하였고, 종자 특성으로는 리 터중, 천립중, 종자의 길이, 폭과 두께를 측정하였다. 수발아 관련 특성으로는 모래묻이법을 이용한 수발아율과 종자 휴면 성과 상관 있는 GI를 조사하였다. 수장과 경수는 년차간 변이 가 없었으며, 리터중은 년차간 및 품종간 차이가 없었고, 이외 나머지 농업형질과 종자 특성 및 수발아율은 년차간, 품종간 및 품종과 년차간 상호작용에서 차이가 있는 것으로 나타났 다. 농업형질은 수발아 특성과 상관이 없었지만, 출수기와 성 숙기는 1수립수 및 종자 길이와 상관을 나타냈으며, 천립중과 종자 길이는 간장과 망장과는 정의 상관을 1수립수와는 부의 상관을 나타내었다. 수장과 경수는 종자 폭 및 두께와 상관을 보였다. 천립중은 수발아율 및 GI와 정의 상관을 나타내었으 며(r = 0.618, P < 0.001, r = 0.525, P < 0.01), 종자의 길이, 폭과 두께 역시 수발아율 및 GI와 정의 상관을 나타내었다. 백립계 품종은 적립계 품종에 비하여 리터중을 제외한 모든 특성에서 높은 값을 나타내었으며, 수발아율과 GI 값도 높게 나타났다. 적립계 품종인 수안밀, 수강밀, 알찬밀과 고소밀은 수발아 저항성이면서 휴면성이 강한 품종으로, 국내 밀 육종 프로그램에서 수발아 저항성 육성 모재로 이용할 수 있을 것 이다. 국내 밀 품종의 수발아 저항성 증진을 위한 계통 선발 에서 종피색과 종실의 무게와 크기도 고려해야 한다.

국산밀을 재배하는 전국 175농가를 대상으로 2년 동안 농업형질과 원맥 특성을 조사 한 결과, 간장, 수장, 경수와이형주율은 년차간 차이를 나타내었다. 시비량이 많았던 남부지역에서 재배된 밀의 간장이 중북부 지역 보다 길었으며, 추비시기는 간장에 영향이 없었지만, 3월 중순 이후에늦게 추비를 준 농가의 수장은 다른 농가에 비하여 길었으나 경수는 감소하였다. 추비 횟수는 농업형질에 영향을 미치지 않는 것으로 나타났다. 농업형질과 마찬가지로 원맥특성도 연차간 변이가 있었으며, 남부지역에서 재배된 밀은중북부지역에서 재배된 밀에 비하여 천립중이 높았으며, 리터중은 낮았으며, 전라북도는 다른 지역에 비하여 낮은 회분 함량과 높은 단백질 함량을 나타냈으며, 전라남도는 다른 지역에 비하여 높은 회분 함량을 나타내었다. 추비시기가 리터중에는 영향을 주었지만 천립중과 회분 및 단백질함량에는 영향을 주지 않았으며, 추비 횟수도 원맥 특성에영향이 없는 것으로 나타났다. 시비량에 따라 간장이 증가하고, 천립중과 단백질 함량이 증가하였으며, 수장과 회분함량은 시비량과 부의 상관을 나타내었다. 단백질 함량은천립중과 정의 상관을 나타내었지만 회분 함량은 천립중 및리터중과 부의 상관을 나타내었다. 간장이 증가할수록 천립중은 증가하고 리터중은 감소하였으며, 수장은 천립중과 부의 상관을 나타내었다. 시비량이 간장 및 원맥 특성에 영향을 미치기 때문에 품종별 용도에 맞는 밀 품종을 재배하기위해서는 적정 시비기술에 대한 농가 지도가 필요하다. 또한, 국산 밀의 생산성 증진과 품질 향상 및 균일성 확보를위해서는 용도별 가공적성에 적합한 품종에 대한 집단 재배단지를 조성하고 철저한 생산 관리와 체계적인 수확 후 관리 구축에 대한 종합적인 고려가 있어야 할 것이다.

식물연구에 있어 다양한 분자마커의 개발은 학문적 그리고 기술적으로 새로운 혁신을 가져왔다. DNA를 이용한 분자마커는 DNA 지문뿐만 아니라 식물의 분류학, 생리학 및 유전공학 등 다양한 분야에서 사용되고 있다. 밀은 옥수수, 벼와 함께 세계 중요 3대 작물 중 하나로서 17,000 Mb의 게놈을 가지고 있어 연구에 많은 어려움이 따르고 있다. 본 연구는 현재까지 보고된 분자마커를 이용한 밀 연구 분야에 관한 보고들을 분석하여 앞으로의 연구 방향을 제시하고자 하였다. 1990년부터 2012년까지 발표된 주요 논문 1,123편을 분석한 결과 유전육종 분야가 38.3%로 가장 높았으며, 분자표지인자와 생리학 분야가 각각 26.9%와 1.5%로 나타났다. 미국을 포함하여 중국, 호주 영국 등 4개국에서 발표한 논문이 전체의 50% 이상을 차지하고 있으며, 이들 국가소속기관 연구소에서 발표된 논문이 전체의 52%로서, 대학보다 활발히 진행되고 있는 것을 알 수 있었다. 특히 밀의 연구는 1992년부터 2007년까지 급격하게 증가하였는데 이것은 밀의 다양한 육종사업과 분자표지인자 기법의 발달이 영향을 미쳤기 때문이다. 그러나 국내 밀의 연구 논문은 88편으로 2008년 이후 대부분 보고되는 것으로 보아 밀의 연구가 매우 늦게 시작된 것을 알 수 있다. 현재까지 국내 밀의 연구는 유전육종 분야에 집중되어 있는 것을 알 수 있다. 국산 밀은 ‘금강’ 등 30 여 품종이 생산량 증가, 재해저항성 증진 등의 목적으로 육성되었다. 예를들어 백립계 제면용으로서 내한성과 붉은곰팡이 중도 저항성을 가지는 ‘한백’, ‘적중’, ‘알찬’, ‘수강’ 등의 품종이 개발되었다. 이와 같이 다양한 목적에 맞는 밀 품종이 육성되어 있기 때문에 이 후의 연구는 용도별 신품종 육성과 함께 국산 밀 품종을 보호하는 방향으로 나아가야할 것이다. 이를 위해선 국산 밀의 기본적인 식물적 특성과 유전적 특성이 확립되어야한다. 최근 국산 밀의 품질 및 품종 판별에 적합한 분자표지인자를 개발하기 위한 다양한 분석방법이 시도되고 있다. 다양한 분자표지인 자 기술 및 개발은 앞으로의 밀 육종사업에 유용한 도구로서 이용가치가 매우 높기 때문에 SSR, ISSR, STS 등의 분자표지인자를 개발하여 국내 밀품종을 보호할 뿐만 아니라, 밀의 수량성 및 고품질과 내재해성 등에 관련된 많은 연구를 통하여 밀의 품질과 수량성 향상에 이용해야 할 것이다.

“Hojoong”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA, Iksan, Korea, during the period from 2006 to 2012. The heading and maturing dates of this variety were May 2 and June 10 in upland, and May 3 and June 7 in paddy field, respectively. It is an It is an awned, semi-dwarf and soft red winter wheat. Culm and spike length of “Hojoong” were 79cm and 9.1cm. It had lower test weight (803 g/ℓ) and 1,000 grain weight (39.5g) than “Keumkang”. It showed resistance to pre-harvest sprouting, which lower rate of pre-harvest sprouting (5.5%) than “Keumkang”. “Hojoong” had lower flour yield (66.7%) and ash content (0.38%) than “Keumkang”. It showed lower protein content (11.3%) and lower SDS-sedimentation volume (34.0㎖) and amylose content (20.5%) than “Keumkang”. It showed higher high viscosity (204BU) and lightness of noodle dough sheet (80.63). It showed different composition in HMW-GS compositios (2.2+12), PPO18 (876bp), GBSS B (null type) and Puroindolines composition compared th “Keumkang”. Average yield of “Hojoong” in the regional adaptation yield trial test was 5.51 ton/ha in upland and 5.10 ton/ha in paddy field. “Hojoong” would be suitable for the area above –10℃ of daily minimun temperature in January in Korean peninsula

“Baechal”, a winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA, Iksan, Korea, during the period from 2006 to 2012. The heading and maturing dates of this variety were May 1 and June 5 in upland, and May 2 and June 8 in paddy field, respectively. It is an It is an awned, semi-dwarf and hard white wheat. Culm and spike length of “Baekchal” were 75cm and 8.7cm. It had lower test weight (794g/ℓ) and 1,000 grain weight (35.7g) than “Keumkang”. It showed moderate to pre-harvest sprouting, which lower rate of pre-harvest sprouting (13.7%) than “Keumkang”. “Baekchal” had similar ash content (0.47%) and protein content (13.1%) to “Keumkang”. It showed lower gluten content (9.6%), SDS-sedimentation volume (38.2㎖) and amylose content (6.5%) than “Keumkang”. It showed higher high viscosity (643BU), water absorption (213%), expansibility of cooking (357%). It showed different composition in HMW-GS compositios (2*), PPO18 (876bp), GBSS (waxy type) and Puroindolines composition compared th “Keumkang”. Average yield of “Baekchal” in the regional adaptation yield trial test was 4.89 ton/ha in upland and 4.83 ton/ha in paddy field. “Baekchal” would be suitable for the area above –10℃ of daily minimun temperature in January in Korean peninsula

‘Jungmo2503’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2011. It was derived from the cross between ‘Dongsanpi81’ and ‘Kangbori’. Among the cross made in 1999, a promising line, SB992028-B-B-B-B-B-2, showed good characteristics in potential forage yield in the yield Trial tested at Iksan in 2007 to 2008 designated as Iksan 449. The line in the Regional Yield Trials (RYT) tested in eight locations around Korea for three years from 2009 to 2011, and was released as the name of ‘Jungmo2503’. It has the growth habit of group Ⅰ, erect plant type, green leaf and hood spike. Its average heading and maturing dates were on May 2, and May 29, respectively, with are similar to check cultivar ‘Yuyeon’. The cultivar had 102cm of culm length, 691 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2503’ was about 10.9 ton ha-1 in dry matter in paddy field. ‘Jungmo2503’ also showed 9.4% of crude protein content, 27.3% of ADF (Acid Detergent Fiber), 49.0% of NDF (Neutral Detergent Fiber), and 67.3% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

Fusarium head blight (FHB), caused by Fusarium graminearum is a major disease problem on wheat and barley in Korea. The objectives of this study were to evaluation of korean wheat cultivars for Type I and Type II resistance to FHB. We screened for Type II resistance in the greenhouse using single floret inoculation and for Type I resistance in the field using spray inoculation. Sumai 3 was used the FHB resistant check. Thirty-two korean wheat cultivars were evaluated for resistance to spread of symptoms within spike. The 2013 field screening with wheat cultivar was located in Kimjae-si Joeonbuk Korea. All plots were inoculated twice. Mist-irrigation was applied to facilitate FHB development. FHB severity was assessed visually 21 days after inoculation on 20 arbitrarily selected spikes per plot. FHB severity was determined as the percentage of symptomatic spikelets from the total of all spikelets. For FHB resistance, the average of FHB severity of Type I exhibited ranging from 21.9% to 77.2% and FHB severity of Type II ranging from 20.8% to 100%. Namhae, Milseong, Geuru, Joen, Anbaek and Sukang were the moderately resistant cultivars while Gobun, Alchan, Dajoong, Eunpa, Shinmichal1, Eunpa and Uri were the most susceptible cultivars for Type II resistance.

‘Jungmo2502’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2011. It was derived from the cross between ‘Samheung/Suwon 300’ and ‘Milyang 100’. Among the cross made in 2000, a promising line, SB00T2018-B-B-B-B-3, showed good characteristics in potential forage yield in the yield Trial tested at Iksan in 2007 to 2008 designated as Iksan 448. The line in the Regional Yield Trials(RYT) tested in eight locations around Korea for three years from 2009 to 2011, and was released as the name of ‘Jungmo2502’. It has the growth habit of group Ⅵ, erect plant type, green leaf and awnless spike. Its average heading and maturing dates were on May 4, and May 30, respectively, with are similar to check cultivar ‘Youngyang’. The cultivar had 98cm of culm length, 607 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2502’ was about 11.0 ton ha-1 in dry matter in paddy field. ‘Jungmo2502’ also showed 8.7% of crude protein content, 21.9% of ADF (Acid Detergent Fiber), 40.7% of NDF (Neutral Detergent Fiber), and 71.6% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

수발아 평가와 GI 평가는 정의 상관을 나타내었고, GI 평가는 년차간 변이를 나타내지 않아서 향후 수발아 저항성 계통 평가에 활용이 가능하지만 품종과 연차간 상호작용에서 변이가 나타나기 대문에 향후 지속적인 평가를 통하여 활용여부를 결정해야 할 것으로 생각한다. 수강밀과 우리밀은 수발아율과 GI 모두 낮게 나타나서 수발아 저항성 자원으로 활용될 수 있으며, 국내 밀 품종에는 없지만 수발아 저항성 외국 자원에서 나타나는 Vp-1Bb와 Vp-1Bf는 수발아 저항성 증진을 위해 도입을 고려해야 할 것이다. 염색체 4AL에 존재하며 외국 밀 품종에서 효과적으로 활용되고 있는 SSR 마커인 ZXQ118와 Xhbe03를 이용하여 국내 밀 품종을 평가한 결과, 국내 밀 품종의 변이 폭이 다양하지 않아 이들 표지 인자를 이용하여 수발아 저항성 품종을 구별하기는 어려웠기 때문에 앞으로 국내 밀에서 수발아 저항성 계통 선발에 활용이 가능한 마커 개발에 대한 연구가 필요하다.

Fusarium head blight (FHB), caused by Fusarium graminearum is a major disease problem on wheat and barley in Korea and around the world. We screened for Type II resistance in the greenhouse using single floret inoculation and for Type I resistance in the field using spray inoculation. Sumai 3 was used the FHB resistant check. Three hundred and seventy lines were evaluated for resistance to spread of symptoms within spike (type II). The 2012 field screening with 300 wheat lines was located in Kimjae-si Joeonbuk Korea. All plots were inoculated twice. The first inoculation was applied at anthesis for wheat. The second inoculation was applied three days after the initial inoculation (dai) for each plot. The inoculum was F. graminearum (GZ3639) prepared at a concentration of 100,000 macroconidai/ml with Tween 20 added as a wetting agent. Mist-irrigation was applied from the first inoculation on May 7 till June 7 to facilitate FHB development. FHB severity was assessed visually 21 days after inoculation on 20 arbitrarily selected spikes per plot. FHB severity was determined as the percentage of symptomatic spikelets from the total of all spikelets observed in these 20 spikes. Based on the field test, we could observe four categories of FHB severity: resistant (R: 0-20%), moderately resistant (MR: 21-40%), moderately susceptible (MS: 41-60%), and susceptible (S: 61-100%). The results showed that forty four lines showed the resistant category on FHB severity between 2.7% and 19.8%. In addition, ten lines showed similar FHB severity compared to Sumai 3 (9.9%).

Whole grain wheat bread and bakery products is highly considered as a functional food due to the high amount of vitamins, minerals, and dietary fiber in whole wheat flour. However, as the lower end-use properties of whole wheat products limit consumer selection, it is required to measure the functionality of whole wheat flour. Thus, it is observed the fermentation properties of bread dough from the composite flour of whole grain and white wheat flour. Whole grain cv. Chokyeong developed in the NICS was ground using a hammer mill with 0.5 mm sieve. The composite flour was prepared by substituting wheat flour with whole grain flour at 15, 30, 45% levels. Breads were prepared using the 100 g composite flour according to a basic straight-dough mehtod (AACC 10-10A) with slight modification. A rheofermentometer was used to measure the maximum dough height (Hm) and the maximum gas formation (H’m) during fermentation. Also, total CO2 production was recorded. The Hm decreased from 38.8 to 27.5 (mm) with increasing whole wheat flour from 15 to 45%. Whereas, the H’m was increased from 61.7 to 60.8 (mm) with whole wheat flour increment. Total CO2 production was also decreased with increasing whole wheat flour ranging 1312∼1164 (ml). The fermentation results could be useful to partly predict the effect of substituting whole wheat flour on the end-use quality of pan-breads.