버섯 다당류는 면역 조절 기능, 항암 및 항산화 활성을 비롯하여 항바이러스 활성과 방사선 스트레스의 경감 등 인체에 유익한 다양한 생리활성을 나타낸다. 본 연구에서는 분홍느타리버섯 (Pleurotus djamor var. roseus Corner)으 로부터 뜨거운 물과 에탄올 침전을 이용하여 5.6%의 수율로 갈색을 띠는 경화된 다당류(CPs)를 순차적으로 추출하였다. 이렇게 추출된 CP는 Diethylaminoethyl cellulose (DEAE) 및 sepharose-6B 컬럼 분리를 통해 4개의 분획을 얻었고 각 분획의 총 글루칸 함량은 각각 76.85%, 2.95%, 75.08%, 1.46%로 밝혀졌다. 이중 가장 높은 수율의 분획 (PP)으로부터 300 mg의 백색 분말이 얻어 졌으며, 박층 크로마토그래피(TLC)와 푸리에 변환 적외선 분광법(FTIR) 의 결과로부터 자일로펜토스 유형의 화합물과 함께 다당류 부분의 존재를 확인하였다. PP의 항산화 활성은 1,1- diphenyl-2-picryl-hydrazyl(DPPH) 자유라디칼 소거 분석 및 슈퍼옥사이드 라디칼 소거 분석을 통하여 높은 활성을 나타냄을 확인하였다. PP분획에는 페놀, 단백질 및 단순 탄수화물이 없는 정제된 베타글루칸이 주 구성성분으로, 정 제된 다당류가 천연 항산화제로 사용될 수 있음을 알 수 있었다.

The fourth Industrial Revolution, known as digital transformation, has made MZ generation to be the focus of the new consumer market, brought about the use of technological platforms a new consumption method. Currently, as various types of content collaboration are emerging that specifically targeting at the MZ generation. Content collaboration are considered an integration of content to create new values through co-existence and co-prosperity. This study identified the characteristics of collaboration of fashion brands from 2018 using literature and online news articles, and identified and classified through case studies of it determined movie content, game and virtual characters. By this research, it shown that collaboration with movie contents have increased the collaborative synergy by using the story in global media content. Collaboration with mobile games was generally used by young casual and sportswear brands. These brands which utilized characters from mobile games popular with to attract more teen consumers and strengthen brand awareness by adding values of high-technology and scarcity to the familiar images. In addition, collaboration with virtual characters has expanded value of the collaborative approach on expanding the range of advanced digital technology, from a promotional strategy during the distribution process through to the use of virtual models. As such, collaboration using the various types of content has developed beyond simple integration of identities among various areas, integrated products or brands that as a new value.

본 연구는 한국근로자를 대상으로 하여 성장욕구가 직무열의와 직무탈진에 영향을 미치는지 조사하였 다. 이와 더불어 부정적 정서조절과 내적 동기가 이들 변수 간의 관계에 영향을 미치는지에 대해서도 검증하였다. 본 연구를 위해 한국의 제조기업에 근무하는 244명의 근로자에게 자기보고식 설문조사를 실시 하였다. 연구결과, 직무열의와 직무탈진에 대한 성장욕구의 직접 효과는 없는 것으로 나타났다. 그러나 이들 변수들 간의 관계는 부정적 정서조절과 내적 동기의 매개역할에 의해 강화되는 것으로 나타났다. 즉 성장욕구와 직무열의의 관계에 대해 부정적 정서조절과 내적 동기는 매개효과를 가졌으며, 성장욕구와 직무탈진의 관계에 부정적 정서조절이 매개효과를 가졌다. 이 결과를 통해 본 연구는 성장욕구와 직무 열의, 직무탈진의 관계에 대한 부정적 정서조절 및 내적 동기와 같은 심리적 특성의 중요성을 확인하였다.

본 연구는 중소기업의 임원보수, 기업성과, 국제화에 대한 소유구조의 역할을 고찰한다. 소유와 경영의 엄격한 분리를 상정한 기존 문헌과 달리 소유와 경영이 중첩되는 임원의 주식소유에 주목한다. 임원 소유지분을 지배주주 소유지분과 분리․취급함으로써 임원보수, 기업성과, 국제화에 미치는 소유지분의 순효과를 임원과 지배주주의 몫으로 분해하여 측정할 수 있다. 이에 2014~2018년 기간 비금융산업에 속하는 국내 상장 중소기업의 공시정보를 활용하여 소유지분 및 임원보수가 기업성과와 국제화에 각각 어떠한 효과를 가져오는지를 주로 분석하였다. 주요 분석 결과는 다음과 같다: (1) 임원보수는 기업성과와 국제화에 직접 영향을 미치는 요인이며, (2) 임원 지분소유는 임 원보수와 양의 상관관계를 통해 간접 경로로 기업성과와 국제화에 영향을 주고 있으나, (3) 지배주주 지분소유은 기업성과 및 국제화에 직․간접 관계가 있다고 판단하기 어렵다. 이 결과를 통해 소 유와 경영의 일치가 기업성과와 국제화에 직․간접적으로 중요한 요소라는 점을 확인할 수 있다.

The Singil Stream, flowing into an artificial lake, Lake Shihwa (South Korea), experiences a strong anthropogenic pressure with continuous organic matter (OM) inputs from rural, urban, and industrial areas. In this study, we investigated suspended particulate matter (SPM) and streambed sediments collected along the Singil Stream in 2014 and 2016, by applying a dual element approach (δ13C and δ15N) to identify OM sources. The SPM and streambed sediment samples from the indusrial area showed higher organic carbon and nitrogen concentrations (or contents) than those from the other areas, with distinctively lower δ15N values. Accordingly, our dual element approach indicates that the industrial area was the predominant OM source influencing OM quality and thus water quality of the Singil Stream flowing into Lake Shihwa during the study periods. However, further studies are necessary to better constrain OM sources in the Singil Stream since OM sources from the industrial area appear to be complex.

This study is an empirical research to find out the effect on the management performance of hidden champions of food manufacturing companies when using blue ocean strategy for new product development. In order to achieve the purpose of this study, we conducted a questionnaire survey on hidden champions in the domestic food manufacturing industry and proceeded empirical analysis. When small and medium-sized enterprises in food manufacturing industries develop a new product, searching for non-customer, rebuilding the market boundary, and linking the external networks have a significant impact on their management performance. However, the fair procedure did not have a significant effect on the management performance. In terms of relative influence, rebuilding the market boundary was most affecting, followed by searching for non-customer and linking the external networks. On the other hand, this study implicated the management performance of hidden champions of food manufacturing industries when new products is developed by using the blue ocean strategy. Obtained results are as follows. If small and medium-sized enterprises of food manufacturing industries develop new products, it will be able to improve the management performance by utilizing strategies such as searching for non-customer, rebuilding the market boundary, and linking the external networks. In particular, the rebuilding the market boundary among the blue ocean strategies has a relatively high impact on management performance.

Porcine epidemic diarrhea virus (PEDV) infects all-age pigs and causes enteric diseases. Genetic diversities in isolates been reported from each country, and those diversities highlighted in pathogenicity and vaccine. In this manuscript, we are reporting of new PEDV isolation in Korea, and with genetic characteristics. Our new isolate belongs to G2b and put the name as CNUP6-2018.

We investigated the effect of moderate- and high-intensity resistance training on hippocampal neurotrophic factors and peripheral CCL11 levels in high-fat diet (HFD)-induced obese mice. C57/black male mice received a 4 weeks diet of normal (control, CON; n = 9) or a high-fat diet (HF; n = 27) to induce obesity. Thereafter, the HF group was subdivided equally into the HF, HF + moderate-intensity exercise (HFME), and HF + high-intensity exercise (HFHE) groups (n = 9, respectively), and mice were subjected to ladder-climbing exercise for 8 weeks. The hippocampal brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) levels were significantly lower in the HF group than in the CON group (p < 0.05). In addition, in the HFME and HFHE groups were significantly higher than in the HF group (p < 0.05). The peripheral CCL11 levels were significantly higher in the HF group than in the CON group (p < 0.05). In addition, in the HFME and HFHE groups were significantly lower than in the HF group (p < 0.05). However, there was no significant difference according to the exercise intensity among the groups. Collectively, these results suggest that obesity can induce down-regulation of neurotrophic factors and inhibition of neurogenesis. In contrast, regardless of exercise intensity, resistance training may have a positive effect on improving brain function by inducing increased expression of neurotrophic factors.

Prolactin is an anterior pituitary hormone involved in various physiological phenomenon including reproduction. The prolactin receptor (PRLR) is detected in diverse tissues such as brain, ovary, placenta and uterus in several mammalian species. A total of 227 pigs [Korean native pigs (KNP) 27; Landrace pigs 29; Korean native pigs x Landrace F1 91; Nanchuckmacdon pigs 80] were used to investigate the allele frequency difference of the prolactin receptor (PRLR) gene among the four pig lines. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with Alu I restriction enzyme was used to determine the genotypes of PRLR. Frequencies of PRLR alleles among the four different pig lines were not significantly different (Chi-square=3.94, DF=3, P=0.27). A total of 40 Nanchuckmacdon pigs were used to investigate the effect of the prolactin receptor (PRLR) gene on total number of piglets born (TNB), number of piglets of alive (NBA) using general linear model implemented in MINITAB software. For TNB, the AB genotype had higher genotypic value (10.61) than the values of AA (9.83) and BB (10.30). Likewise, the AB genotype had higher genotypic value (8.96) than the values of AA (8.18) and BB (8.90) for NBA. However, these associations of the PRLR gene with TNB and NBA were not statistically significant. In conclusion, it is necessary to increase the sample size for investigating the effect of the PRLR gene on TNB and NBA in pigs.

Blood types in pigs are divided into two types, A and O type. It is important to select and breed O-type pigs that have no hematologic rejection, because the blood type of the donor is an important factor causing immune rejection in xenotransplantation. Therefore, the gene that determines the blood type is GGTA1(glycoprotein, alpha-1,3-galactosyltransferase 1), which generally belongs to the ABO blood group. This study was carried out to develop a simple and accurate method by analyzing the structure of GGTA1 gene which determines blood type. A primer was designed to allow easy identification of the blood type of the target in the first and second deletion regions of intron 7 to distinguish between the A and O genotypes. That is, for the purpose of identifying the blood type using length difference after the PCR, the forward and reverse primers were designed in the highly conservative region. As a result, a pair of primers were prepared and PCR amplification was performed to distinguish three types of genotypes, AA, AO, and OO, using the length difference by electrophoresis. Using the above primers, the parents and their offspring were compared with each other to confirm the correct genetic pattern. And, in four pig breeds, the genes were amplified and the genotype could be correctly identified. In this study, we could diagnose the blood type of AA, AO, OO genotype of pigs by using primer of INDEL region. Especially, since it is possible to diagnose the genotype by the length difference, it is possible to diagnose it quickly and accurately from the gene amplification to the genotype reading

2017년 경북지역은 약 700ha 이상의 면적에서 미국선녀벌레의 피해를 입었다. 그 중 600ha의 피해면적이 떫은감 주요 생산지인 청도군에서 발생하였다. 미국선녀벌레의 효율적인 방제를 위해 2018년 5월 상순에 침투이행성 약제를 토양에 처리하여 약충이 부화하기 전감나무 수체 내 약제를 이행시켜 방제효과를 조사하였다. 토양입제처리의 방제효과 조사를 위하여 토양입제 단독처리(5월 상순), 경엽살포 단독처리(6월 중순), 토양입제(5월 상순) 및 경엽살포(6월 중순) 체계처리를 비교하였다. 조사결과 토양입제 단독처리 시 약제처리 후 약 70일 이상 90%이상의 방제효과가 지속되었고, 경엽살포 단독처리 시 약제처리 후 약 2주 동안 90%이상의 방제효과를 보인 뒤 방제효과가 감소하였다. 또한 토양입제 및 경엽살포 체계처리 시 약제처리 후 약 70일 동안 95%이상의 높은 방제효과를 보였다.

The purpose of this study is to conduct a basic research for the development of a program, PEP-NKMF (Parents Empowerment Program in North Korea Migrant Family). A total of 20 teenager North Korean defectors from an S school in Seoul city and five North Korean defector teachers at this school were surveyed through questionnaires and in-depth interviews from March 6 to 31, 2017. The results were analyzed using Nvivo 11.0, which is a representative method of qualitative analysis for forming nodes by tree structure. The results showed that the teenage North Korean defectors requested the family relationship competence, adaptation, interest and love from their parents. North Korean defector teachers requested the transformation of the value and the practical love. This study is meaningful in that the essential requirements of the parental competence building program, which is to be provided to North Korean defector parents, are analyzed based not on theories but on teenage North Korean defectors and North Korean defector teachers.

This study analyzed variations of learning stress by comparing the salivary cortisol levels of students who participated in Earth Science inquiry activities. The cortisol concentrations between the pre- and post-inquiries of the sample of 34 university students, who had taken the course of ‘Basic Earth Science and Experiments’, were analyzed. The Earth Science inquiries consisted of geology and astronomy activities. The observational geology activities consisted of a session of ‘structure contours and map patterns’ and the cognitive astronomy activities consisted of a session of ‘representations of horizontal and equatorial coordinates’. These Earth Science inquiry activities were found to cause students to have anxiety, and the thought processes that these activities involved were found to cause learning stress. The variations in cortisol concentrations of students increased by 1.6±5.9 ng mL−1 after conducting observational activities in geology compared with 2.1±6.2 ng mL−1 after doing cognitive activities in astronomy. The analysis of the observational activities in the geology inquiry activities indicated that they were consistent with low levels of learning stress. Conversely, the analysis of the cognitive activities in the astronomy inquiry activities showed significant individual variations in cortisol concentrations. Furthermore, individual differences in cognitive ability were reflected in the astronomy inquiry activities. While students, who received high scores, exhibited low levels of stress in the geology inquiry activities, they showed high levels of stress in the astronomy inquiry activities. It was concluded that, in the case of students with high scores in the study, the level of learning stress increased due to the raised anxiety in cognitive inquiry activities. In contrast, students, who received low scores in the study, exhibited high levels of stress in the geology inquiry activities, and low levels of stress in the astronomy inquiry activities.