본 연구에서는 의류용 직물의 소리와 변환색채의 특성을 분류하고, 역학특성과의 관계분석을 통해 직물의 역학적 특성과 소리 그리고 변환색채와의 관계를 상관분석하고자 하였다. 다양한 의류용 직물의 스치는 소리를 녹음한 후 소리의 물리량으로 군집분석을 하였으며, Okamoto와 Mori의 색채변환 프로그램을 이용하여 직물의 소리를 색채로 변환하였다. 직물의 소리는 각각 silky, crispy, paper-like, worsted, flax의 다섯 그룹으로 나뉘었다. Silky는 매끄럽고 부드러운 견직물 등으로 구성되었으며, LPT(소리의 음압), δf, ARC값이 군집 중 가장 낮아 심리 음향학적 loudness(z)와 sharpness(z)가 제일 낮은 조용한 직물들로 나타났다. 변환된 색채는 홍색 계열이었으며 색채 빈도수가 가장 많았다. raper-like는 뻣뻣하고 파삭파삭한 직물들로 LPT, δf ARC값이 군집 중 가장 높아 loudness와 sharpness가 높은 시끄러운 직물들로 나타났다. Worsted는 모직물과 모직물 느낌의 직물들로 LPT, δf, loudness값이 paper-like다음으로 높은 값을 보였으며 sharpness는 유사했다. Paper-like와 worsted의 변환된 색채는 청색 계열이 주류를 이루었으며 매우 낮은 색채 빈도수를 나타냈다. Crispy는 표면이 까실까실한 느낌의 직물들로 loudness(z)와 sharpness(z)의 값은 높지 않았으나 변환된 색채는 다양한 색채계열과 빈도수를 나타냈다.

The objective of this study was to identify the anti-oxidation, astringent, and inhibition effects of wild Ligularia fischeri on hyaluronidase and angiotensin conerting enzyme (ACE). In order to identify the total phenolic compound (TPC), various solvents were used for extraction showing hot water extract with the highest value of 14.42 GAE mg/g. In addition, ABTS radical scavenging activity measurements revealed an anti-oxdiation effect of 98.64-99.84% a hot water extract concentration of 50-200 μg/mL and a radical scavenging activity of 95.14-98.96% at a 60% ethanol extract content. If expressed in antioxidant protection factors (PF), the hot water extract showed 0.59-1.02 PF and the 60% ethanol sample displayed 0.30-0.74 PF. To identify the bio-activity effect, the hyaluronidase inhibition effect was determined as 4.66-35.00% in a 50-200 μg/mL hot water extract. Considering ACE inhibition effect, the hot water extract and 60% ethanol sample showed 0-64.24% and 46.12-69.64% inhibition effect, respectively. Lastly, when taking into account the astringent effect, the hot water extract with 50-200 μg/mL TPC concentration showed 15.68-26.92% and the 60% ethanol sample with an equal concentration exhibited 49.48-86.84%, which indicates the possibility to apply this product as a cosmetic source for pore contraction. Therefore, wild Ligularia fischeri extract can be used for anti-inflammation, high-blood pressure prevention, and as a source for health functional food with anti-oxidative properties.

Background : This study was conducted to serve as a basis for the production of high quality organic cultivation Platycodin grandiflorum Ridix roots by analyzing the content of saponins according to organic fertilizer for organic farming. Methods and Results : Platycodin grandiflorum Ridix was treated by with biodegradable film, rice husks, and sawdust, non-mulching (hand weeding). A root of Platycodon grandiflorum were analyzed saponins using HPLC harvested on October. Content of Plantycodin D3 was an average of 68.5 ㎎/100g, which appeared as 111.2 ㎎/100g highest content in the sawdust treated. Deapioplatycodin D showed the highest 48.1 ㎎/100g in the sawdust treated. The content of Platycodin D was shown to 246.2∼373.8 ㎎/100g, the highest values were observed in non-mulching, it showed the lowest value in the rice husks treated. The content of Polygalacin D showed up to 156.0 ㎎/100g in the non-mulching as the biggest difference, the lowest value were 30.1 and 32.2 ㎎/100g in sawdust and rice husks treated. Large differences between fertilizers was not showed, but the overall saponin content of Platycodin grandiflorum Ridix were low in value compared to the control than organic fertilizers. Conclusion : Large differences between mulching materials was showed, thus the overall saponin content of Platycodin grandiflorum Ridix was highest in value compared to non-mulching than sawdust treatment. Mulching Platycodin grandiflorum Ridix with sawdust was an effective method for improving saponin contents.

Background : This study was performed to know the effect of mulching materials on the growth and root characteristics of Platycodin grandiflorum Ridix. Methods and Results : Platycodin grandiflorum Ridix was treated by with biodegradable film, rice husks, and sawdust, non-mulching (hand weeding). To measure the fresh weight and length, thickness and the number of fine root of the Platycodin grandiflorum Ridix roots harvested in October. Root length appeared in the biodegradable film and sawdust were 27.5 and 26.7㎝ longest, rice husks was the shortest 22.0㎝. Diameter at the thickest showed 30.9 ㎜ in biodegradable film, the following phrase appeared sawdust and rice husks application. The number of fine-root was investigated in biodegradable film most piece 36.0. Fresh weight, which is directly related to yield were biodegradable film to 130 g the highest in the number of fine-root and the longest root length. Conclusion : For the increasing the yield that Pharmacological effect, biodegrada

Background : The root of Platycodin grandiflorum has been widely used as a crude drug or a dependent on the cultivation area and cultivation method. Therefore, this study was conducted to know the effect of organic fertilizer on the changes of antioxidents and antioxidant activity of Platycodin grandiflorum Ridix. Methods and Results : As basal fertilizer were treated before transplanting Platycodin grandiflorum Ridix with chemical fertilizer(Super21, 21-17-17, Namhae Chemical), mixed organic fertilizer, bacterial culture, fermented oil cake and manure in each trial of nitrogen based on 3㎏/10a criteria. The total polyphenol content was highest values were 15.5mg/g appear in the mixed organic fertilizers, the lowest were 9.1mg/g appeared in the manure and control. Flavonoid content showed the highest 15.3 mg/g in the mixed organic fertilizer, and investigated at the lowest 5.0 mg/g in the fermented oil cake. ABTS radical scavenging activity was the highest in was 65.7 mgTE/g in the chemical fertilizer, showed the lowest value in the control to 13.0 mgTE/g. Therefore, both flavonoid and polyphenol content showed a high value on organic fertilizers. Conclusion : Therefore, both flavonoid and polyphenol content showed a high value on organic fertilizers. It is determined that to have researches for a variety of antioxidants by the organic fertilizers made in the future.

Background : This study was conducted to serve as a basis for the production of high quality organic cultivation Platycodin grandiflorum Ridix roots by analyzing the content of saponins according to organic fertilizer for organic farming. Methods and Results : As basal fertilizer were treated before transplanting Platycodin grandiflorum Ridix with chemical fertilizer(Super21, 21-17-17, Namhae Chemical), mixed organic fertilizer, livestock manure, bacterial cultures and fermented oil cake in each trial of nitrogen based on 3 ㎏/10a criteria. A root of Platycodon grandiflorum were analyzed saponins using HPLC harvested on October. Content of Plantycodin D3 was an average of 40.6 ㎎ /100g, which appeared as 48.1 ㎎/100g highest content in the bacteria cultures treated. Deapioplatycodin D showed the highest 70.9 ㎎/100g in the control, the rest was investigated 28.1∼54 ㎎/100g. The content of Platycodin D was shown to 327.4∼373.8 ㎎/100g, the highest values were observed in mixed organic fertilizer, it showed the lowest value in the control. The content of Polygalacin D showed up to 336.7 ㎎/100g in the manure as the biggest difference, the lowest value were 54.5 and 74.2 ㎎/100g in bacterial cultures and control. Large differences between fertilizers was not showed, but the overall saponin content of Platycodin grandiflorum Ridix were low in value compared to the control than organic fertilizers. Conclusion : For the increasing the yield that Pharmacological effect, organic fertilizer is effective to increase the number of fine-root high which high contented with saponins.

Background: Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. Since ancient times, Chrysanthemum indicum L. had been widely used as drug and tea. From 2000, breeding began and the three varieties "Manhyang", "Geumhyang", Gamro" were developed so far. As demand has increased, growth traits of "Wonhyang" has been tested and fostered to report growth characteristics of "Wonhyang". Methods and Results: It is used New variety "Wonhyang" that were forstered in Gyeongsangnam-do Agricultural Research & Extension Services. After going through selection, proliferation and characteristics of CI0901, production performance test carried out in 2012~2013. As a result, it had named as "Gamkuk 4" in high quality, quantity and of strong in the disease. Local adaptability trial test carried out in 2014~2015. Conclusion: "Wonhyang" was a good growth in plant height, lengths of branches. Leaf color is light green, flower color is light yellow. Disease and pest resistance of "Wonhyang" is relatively better than "Manhyang". Since plant height is small and plant width is big, "Wonhyang" is available for medicine, food and landscaping. As a result of local adaptability trial test, Yield(131.1㎏/10a) of three region Hamyang,Hapcheon,Changnyeongis 10% higher than "Manhyang". Based on the results of this study, "Wonhyang" was to be registered for a new breed through the breed fostering council.

Background : This study was performed to know the effect of organic fertilizers on the growth and root characteristics of Platycodin grandiflorum Ridix. Methods and Results : As basal fertilizer were treated before transplanting Platycodin grandiflorum Ridix with chemical fertilizer(Super21, 21-17-17, Namhae Chemical), mixed organic fertilizer, bacterial cultures and fermented oil cake, manure in each trial of nitrogen based on 3 ㎏/10a criteria. To measure the fresh weight and length, thickness and the number of fine root of the Platycodin grandiflorum Ridix roots harvested in October. Root length appeared in the mixed organic fertilizer and chemical fertilizer were 25.3 and 24.0 ㎝ longest, oil cake was the shortest 15.3㎝. Diameter at the thickest showed 26.6 ㎜ in chemical fertilizer, the following phrase appeared bacterial cultures and mixed organic fertilizer application. The number of fine-root was investigated in mixed organic fertilizer and chemical fertilizer most pieces 20.0 and 17.0. Fresh weight, which is directly related to yield were mixed organic fertilizer to 55.7 g the highest in the number of fine-root and the longest root length. Conclusion : For the increasing the yield that Pharmacological effect, organic fertilizer is effective to increase the number of fine-root high which high contented with saponins.

Cysteine protease (CP) is one of the well-studied proteolytic enzymes in plants. This class of protease has been implicated in various physiological aspects of developmental stages in plants including seed germination, senescence, and disease immunity. A handful of studies assigned plants cysteine protease in different molecular battlefield under a few selected pathosystems, and initially extricated complex molecular mechanism of resistance. However, its potential use as an agent of resistance to diseases in rice has never been explored. This study demonstrates the function of CP specifically in rice - Xanthomonas oryzae pv. oryzae (Xoo) pathosystem. The CP -encoding full-length cDNA was cloned from Brassica rapa and transformed into japonica rice cv. ‘Gopumbyeo’. The gene was overexpressed under the control of CaMV35S promoter in pFLC vector. Blast analysis of the conserved domain of the gene confirmed its affinity to Peptidase_CIA family. RT-PCR analysis showed that the gene was constitutively expressed in all tissues tested. Regulation of rice resistance through cysteine protease activity is evident in overexpression lines which exhibited an enhanced resistance to four Korean Xoo isolates. Further analyses will be carried out to uncover the specific role of CP in rice-Xoo interaction.

There is a great consideration on rice eating quality aside from improving its tolerance to various stresses. High yielding and pest and disease tolerant rice is highly desirable but it is more commercially important if it also has a high eating quality. There are various factors contributing to the good eating quality of rice. This study focuses on modifying the expression of GBSS1 genes which are responsible for amylopectin and amylose synthesis in rice by using RNAi and antisense techniques. We have developed 40 transgenic plants with RNAi-GBSS1 gene and 60 transgenic lines with antisense-GBSS1 gene. The transgenic plants show diverse amylose contents in rice seed. We selected candidate lines according to PCR, RNA expression and amylose contents. A semi-quantitative RT-PCR was carried out to measure the expression level of GBSS1 gene at several time points after the flowering of transgenic plants. The expression level of GBSS1 gene in rice grains decreases over time and the mRNA expression among the transgenic plants were lower compare to its wild type. In the SEM analysis, the starch granule of wild type Gopumbyeo has very large structures accompanied with small ones around the area. However, the starch structures in transgenic plants were smaller and more uniform in size and shape throughout the viewing area

An increasing preference for good eating quality of rice among consumers has become one of the important considerations in rice breeding. Amylose content of starch is one of the important factors of rice eating quality. Amylose composition is determined by the relative activity of soluble starch synthase (SSS) and granule-bound starch synthase (GBSS). This study focuses on modifying the expression of SSS1 gene which is responsible for amylopectin and amylose synthesis in rice by using RNA interference (RNAi) and antisense technology. The transgenic rice plants showed various amylose content (9-17%) in rice seed. Candidate rice lines were selected according to PCR, RNA expression and amylose contents analyses. A semi-quantitative RT-PCR was carried out to determine the expression level of SSS1 gene at several time points after the flowering of transgenic plants. Downregulation of SSS1 gene in transgenic rices was evident in the decreasing expression in rice grains over time. Accordingly, SEM micrographs analysis revealed uniform size with smooth curves starch granules in downregulation rice lines, in contrast with the non-uniform granules in wild type.

Since global climate changes drastically, pre-harvest sprouting (PHS) is expected to pose serious problems in rice production. CBL-interacting serine/threonine protein kinases (CIPKs) have been implicated to play important role in regulating various abiotic stresses such as cold, salinity and drought. In this study, to understand the function of this gene under pre-harvest sprouting in rice, a cDNA clone encoding CBL-interacting protein kinase 15 (CIPK15) was isolated from rice flowers. This gene is 2,818 bp long with 1,332 bp coding region that encodes a polypeptide of 443 amino acids. We constructed a recombinant vector carrying the OsCIPK15 under the control of the CaMV 35S promoter and Tnos terminator and transformed into rice using Agrobacterium tumefaciens. Insertion of the gene was verified in transformants using HPT resistance test and genomic PCR. Transcriptional profiling using tissues of wild type, Gopum, revealed expression of the gene in whole plant tissues with level of expression highest in the seeds suggesting possible role in dormancy. Comparative expression analysis of the gene in transgenic and wild type through semi-quantitative RT-PCR and real-time PCR showed higher expression in transgenic rice lines. Moreover, screening in the mist chamber showed overexpression lines that were resistant to the PHS. This result suggests the involvement of OsCIPK15 in the regulation of pre-harvest sprouting.

Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. Exposure of plants to cadmium (Cd) has been reported to decrease GS activity in maize, pea, bean, and rice. To better understand the function of the GS gene under Cd stress in rice, we constructed a recombinant pART vector carrying the GS gene under the control of the CaMV 35S promoter and OCS terminator and transformed using Agrobacterium tumefaciens. We then investigated GS overexpressing rice lines at the physiological and molecular levels under Cd toxicity. The GS activity along with mRNA expression were found higher in transgenic than in wild type plants. And this is validated by the low malondialdehyde contents observed 10 days after treatment. GS overexpression in rice resulted in the modulation of expression of enzymes responsible for membrane peroxidation, which may result in the sudden death of plants. Our results thus describe the features of a transgenic rice plants with enhanced tolerance to Cd toxicity.

Amylopectin composition is determined by the relative activity of soluble starch synthase (SSS) and granule-bound starch synthase (GBSS). Soluble starch synthase and starch branching enzymes are major determinants for the synthesis of amylopectin while GBSS1 is responsible for amylose synthesis in vivo. The formers are made of linear and branched molecules and the latter is composed of highly branched molecules. To increase the palatability of rice, down-regulation of amylose synthesis by antisense and RNA interference (RNAi) could be excellent and powerful tools for controlling the starch composition which is responsible for grain eating quality. The goal of this study is to generate breeding lines with lower amylose content relative to its wild type. This study also reports the results of the two down-regulating technology in lowering the amylose content of rice grain. Furthermore, this study elucidates the effect of using antisense and RNAi for SSS1 and GBSS1.

A cDNA clone encoding CBL-interacting protein kinase 1 (CIPK1) was isolated from Chinese cabbage seedlings. The gene, BrCIPK1 consisted of 1,982 bp long with 216 bp of the 5’-untranslated region (UTR), 1,509 bp of the coding region and 257 bp of the 3’-UTR. It is highly conserved CBL-interacting module with absolutely conserved domain among the 15 amino acid NAF domain of the 15 related genes. Southern blot analysis showed a single copy number. BrCIPK1 gene was localized in the cytoplasm and peripheral region in the plant cell which is highly expressed in seedling of rice and in the shoot and pistil of Arabidopsis. Analyses of gene expression on Ubi-1::BrCIPK1 rice lines was differentially accumulated by cold, salinity and drought, indicating its biological roles in the multiple stress response pathways in plants. Further, the expression of BrCIPK1 is hijacked by rice calcineurin-B-like protein (OsCBL5). Moreover, mRNA expression of P5CS1, a gene responsible for proline biosynthesis is regulated by the BrCIPK1 during abiotic stresses resulting to improved accumulation of proline. The interaction of BrCIPK1 with OsCBL5 along with the regulation of P5CS1 explained the enhanced tolerance of transgenic rice. This gene could be used in the development of rice varieties with enhanced tolerance to abiotic stresses.

MYB-like domain (MLD) gene is a transcription factor that plays a diverse role in plant development and in response to abiotic stresses. In this study, we isolated and developed CaMV35S::OsMLD rice lines and determined its expression pattern under abiotic stresses. It has Myb_CC_LHEQLE superfamily similar to most transcription factor genes but with a very unique binding domain of SHLQKYR in the C-terminal region. Overexpressing rice lines showed enhanced tolerance to salinity with elevated mRNA transcript. Additionally, mRNA transcripts were up-regulated by ABA, H2O2 and dehydration stresses. Further investigation in the enhanced tolerance to salinity showed an increased accumulation of proline and a decreased in malondialdehyde contents indicating that OsMLD gene may be involved in the regulation of proline and osmolytes during abiotic stresses. These results showed that OsMLD gene could be used in the development of rice intended for soil with salinity-related problem.

UDP-glucose 4-epimerase (UGE) catalyzes the reversible conversion of UDP-glucose to UDP-galactose. The gene, named BrUGE1, isolated from a Chinese cabbage had a total length of 1,328 bp that contains a single open reading frame (ORF) of 1,056 bp which encodes a polypeptide of 351 amino acid residues with a calculated mass of 39.0 kDa. Sequence analysis of BrUGE1 protein has the characteristic of an active site tetrad and NAD-binding motif (typically TGXXGXXG) of the extended short chain dehydrogenase/ reductase (SRD) superfamily. Expression analysis showed that BrUGE1 is tissue specific and highly expressed in stem of rice plant. Interestingly, BrUGE1 mRNA was highly accumulated by drought stress with significantly higher amount of soluble sugar. Morphological evaluation showed an increase in yield by 27%. Panicle length, number of productive tillers/hill, and filled spikelets were significantly increased by 17~20% compared to the wild type Gopum. Moreover, the growth of the wild type Gopum seedlings on galactose was increasingly inhibited with a decrease in UDP-glc epimerase 1 expression compared to the transgenic rice lines. In the Ubi-1::BrUGE1 lines, the increase of UDP-glc epimerase 1 expression was apparently sufficient to overcome the toxic effects of galactose. Taken together, the Ubi-1::BrGUE1 rice lines increased yield probably by increasing the rate of filled grains. The enhanced drought tolerance may be due to the induction of soluble sugar which may act as osmolyte to compensate dehydration during drought stress.

A cDNA clone encoding CBL-interacting protein kinase 1 (CIPK1) was isolated from Chinese cabbage seedlings. The gene, BrCIPK1 consisted of 1,982 bp long with 216 bp of the 5’-untranslated region (UTR), 1,509 bp of the coding region and 257 bp of the 3’-UTR. It is highly conserved CBL-interacting module with absolutely conserved domain among the 15 amino acid NAF domain of the 15 related genes. Southern blot analysis showed a single copy number. BrCIPK1 gene was localized in the cytoplasm and peripheral region in the plant cell which is highly expressed in seedling of rice and in the shoot and pistil of Arabidopsis. Analyses of gene expression on Ubi-1::BrCIPK1 rice lines was differentially accumulated by cold, salinity and drought, indicating its biological roles in the multiple stress response pathways in plants. Further, the expression of BrCIPK1 is hijacked by rice calcineurin-B-like protein (OsCBL5). Moreover, mRNA expression of P5CS1, a gene responsible for proline biosynthesis is regulated by the BrCIPK1 during abiotic stresses resulting to improved accumulation of proline. The interaction of BrCIPK1 with OsCBL5 along with the regulation of P5CS1 explained the enhanced tolerance of transgenic rice. This gene could be used in the development of rice varieties with enhanced tolerance to abiotic stresses.