To obtain some information on the change of antioxidant components of seeds during grain filling stage as affected by the sowing dates, lignan compounds were investigated according to days of flowering under different sowing dates. Sesamin and sesamolin contents showed significantly different by days of flowering and varieties. Both of sesamin and sesamolin content increased after flowering and reached highest at 40 days of flowering, but they started to decrease thereafter. Sesamin and sesamolin contents of sesame seeds changed with sowing dates. Generally, late sowing date of May 30 showed relatively higher accumulation rate of sesamin and sesamolin contents rather than other sowing dates, but overall patterns were a little different by varieties and lignan compounds. In case of variety Yangbaek, sesamin and sesamolin contents showed relatively higher at sowing date of May 30, but variety Yanghuck showed higher sesamin and sesamolin contents till 20 days of flowering when sowing date of May 30, but it showed to change that both lignan contents were relatively higher under sowing date of May 10.

NIR spectroscopy combined with multivariate analysis after the appropriate spectral data pre-treatment has been proved to be a very powerful tool for judgment of the relative pattern of the objects that have very similar properties. In this study, 500 GMO soybean seeds and, 500 non-GMO ones were measured in NIR reflectance mode. Principal component analysis (PCA), and discriminant analysis (DA) were applied to classify soybean with different genes into two groups (GMO and non-GMO). Calibrations were developed using DA regression with the cross-validation technique. The results show that differences between GMO and non-GMO soybeans do exist and excellent classification can be obtained after optimizing spectral pre-treatment. The raw spectra with DA model after the second derivative pre-treatment had the best satisfactory calibration and prediction abilities, with 97% accuracy. The results in the present study show NIR spectroscopy together with chemometrics techniques could be used to differentiate GMO soybean, which offers the benefit of avoiding time-consuming, costly and laborious chemical and sensory analysis.

Black soybeans, which have been widely utilized as food and as material for Oriental medicine, contain anthocyanins in the seed coat. Soybean seeds with black seed coat and green cotyledon are called “Seoritae” in Korea. Korean customer prefer “Seoritae” to normal black soybean with yellow cotyledon. The pigments contained in green cotyledon of black soybeans were chlorophylls and lutein. These constituents function as antioxidant and protect humans against diverse damages. The purpose of this study was to investigate the most suitable extraction condition and method of green cotyledon pigments in black soybeans. The green cotyledon pigments were analyzed on RP-HPLC with C18 column using gradient system. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 100% MeOH, and mobile phase B, consisting of 100% EtOAc. Among the 4 kinds of extraction method using methanol and/or acetone solution, ultrasonic extraction at 30 min. using acetone solution was most suitable extraction condition for simultaneous analysis of chlorophylls and lutein in soybean with green cotyledon.

This experiment was carried out to clear optimum planting time for yield enlargement by comparing yield with dry matter at Miryang from 2005 to 2006. Variety used were Daewon, Daepung, Daol and Cheongja 3. Seeding was performed on April 30 to June 30 with 20day intervals and planting density was 142,857 palnts/ha (70×20㎝, 2plants per hill) with black vinyl mulching. In R2～R4 stages, crop growth rate(CGR) was largest on June 10 seeding. Although net assimilation rate(NAR) increased as seeding date was delayed, NAR on June 10 seeding was the highest throughout the growth period. Positive relationships were observed between NAR from R2 to R4 the ratio of pod to total dry matter weight. The LAI and total dry matter weight at the R4 stage was higher at earlier seeding date. In the case when seeding date was delayed, the ratio of leaf(source) and pod(sink) increased. From the comparison of all factors such as dry weight, optimum LAI, and of source to sink, the optimum planting date for high seed production was June 10. The highest yield was recorded with on June 10 seeding. Also, this seeding date showed high ratio of seed yield to dry matter in R2 stage. Difference between surveyed pod no. and theological pod no. which was calculated by dry matter ratio compared with standard seeding date(June 10) at R2 stage was the smallest in June 10 seeding.

This study was carried out to obtain general agronomic characteristics and identify its relationship with seeding date for high quality soybean seed production. One hundred(100) domestic soybean varieties were used and seeding date were scheduled at April 30 and May 30. This experiment was carried out at YARI, NICE situated at Miryang from 2005 to 2006. Maturity Group(MG) was classified by Kweon et al's method (1974). Correlation analysis of major agronomic characters showed positive relationship between days to flowering and growth days, seed weight and lodging in MG ,seed crack and growth days in Ⅳ～Ⅴ MG0～Ⅲ,seed crack and days to flowering in MGⅣ～Ⅴ and MGⅥ～Ⅶ, seed crack and lodging in MGⅣ～Ⅴ and MGⅥ～Ⅶ, seed crack and seed weight in MGⅣ～Ⅴ and MGⅥ～Ⅶ, purple seed and growth days in MGⅣ～Ⅴ , purple seed and seed weight in MGⅥ～Ⅶ, phomopsis and growth days in MGⅣ～Ⅴ and MGⅥ～Ⅶ, and phomopsis and purple seed in MGⅣ～Ⅴ and MGⅥ～Ⅶ. In contrast, a negative relationship was observed between seed weight and lodging in MG0～Ⅲ. Correlating yield and major characters revealed negative relationship between days to flowering and growth days in MG0～Ⅲ and MGⅣ～Ⅴ, whereas positive relationships were obtained on MGⅥ～Ⅶ seeded on April 30. Lodging, seed weight and seed crack were all negatively correlated with yield in MGⅣ～Ⅴ and MGⅥ～Ⅶ

This experiment was carried out to examine seed quality as affected by enviromental cutivation for the production of high quality domestic soybean variety at Miryang (altitude : 12m) and Muju(altitude : 600m). Variety used were Daewon, Daepung, Daol and Cheongja 3. Seeding was performed on April 30 to June 30 and planting density was 142,857 palnts/ha. Seed weight across regions was lighter as seeding date was delayed. Opposite trends between seed crack and seed weight was obtained. Seed crack decreased as seeding date was delayed and have higher occurrence in Miryang than in Muju. Perple seed and phomopsis decayed seed decreased as seeding date was delayed. There was a significant negative correlation between seed weight and mean temperature from R2 to R4 stages in all variety. Seed weight increased as mean temperature from R2 to R4 stage decreased to 22.0℃, which was greatly manifested on Daewon variety. At maturity time, Daewon variety had an average increase of 1.79g as mean temperature decreased by 1℃. Seed weight of Daewon obtained a significant negative correlation with mean temperature at R4～R6 stage and on rainfall at R4～R6 stage. Mean temperature and rainfall were able to estimate 100 seed weight of Daewon and Daepung variety with 98.1% and 96.6% probability, respectively. Phomopsis occurrence in late maturity type was closely related to rainfall during the R4～R6 stages.

This study was carried out to classify Korean soybean varieties based maturity group. One hundred (100) domestic soybean varieties ( 56 for fermentation, 26 for sprouting, 17 for mixed with rice) were used and seeding date was scheduled at April 30. This experiment carried out at Yeongnam Agricultural Research Institute, NICE situated at Miryang from 2005 to 2006. Classification of maturity group in 100 soybeans was followed by Kweon et al's method (1974) The main difference between this experiment and that of Kweon et al's method (1974) were the planting region and planting date. This experiment was conducted on April 30 at Miryang while Kweon et al. was conducted on May 23 in Seoul. Results of maturity grouping of Korean soybean varieties showed that Keunol, which was represented among 3 varieties, belonged to Group 0, Seonnok and Danmi in GroupⅡ, Shinrok in GroupⅢ, Seonyu, represented by 17 cultivars, in GroupⅣ, Taekwang, having 44 cultivars in GroupⅤ, Daewon, represented by 25 cultivars in GroupⅥ, and Kwangdu and Keumdu in GroupⅦ.

Application of genotype by environment (G~;~times~;E ) interaction would be used for identifying optimum test condition of the varietal adaptation in the establishment of breeding purpose. Yield and yield components were used to perform additive main effect and multiplicative interaction (AMMI) analysis. Significant difference for G~;~times~;E interaction were observed for all variable examined. For yield, 0.18 of total sum of squares corresponded to G~;~times~;E interaction. Correlation analysis was carried out between genotypic scores of the first interaction principal component axis (IPCA 1) for agronomic characters. Significant correlations were observed between IPCA 1 for yield and capsule bearing stem length (CBSL), number of capsule per plant (NOC). The biplot of grain yield means for IPCA1 which accounted for 34% of the variation in total treatment sums of squares showed different reaction according to G~;~times~;E interaction, genotypes and environments. Taegu showed relatively lower positive IPCA1 scores, and it also showed smaller coefficient variation of yield mean where it is recommendable as a optimal site for the sesame cultivar adaptation and evaluation trial. In case of variables, Yangbaek and M1 showed relatively lower IPCA1 scores, but the score direction showed opposite each other on the graph. Ansan, Miryang1, Miryang4, and Miryang6 seemed to be similar group in view of yield response against IPCA1 scores. These results will be helpful to select experimental site for sesame in Korea to minimize G~;~times~;E interaction for the selection of promising genotype with higher stability.

A new peanut variety “eonan”(Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was developed from the cross between the erect short stem cultivar “P9128” and the high-yielding cultivar “aekwang” “eanan”which is Virginia plant type has 18 branch number per plant with early maturing and long-ellipse shaped large kernel. Each pod has two grains with brown testa and 100 seed weight was 87 g in the regional yield trials (RYT). Especially this variety has resistance to lodging owing to short stem. The free sugar and tannin content of fresh peanut are 3.7% and 0.48%, respectively, similar to check variety. In the regional yield trials “eonan”was outyielded than check variety by 14% with 8.59 MT/ha for fresh pod and by 7% with 3.75 MT/ha for grain.

A new peanut variety “ungsan”(Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was derived from a cross between a short stem cultivar “atonoka”and a high-yielding local cultivar “echeon” “ungsan”is the Virginia plant type. It has 18 branches per plant and 40 cm of main stem height. Each pod with long-ellipse shaped large kernel has two grains with light-brown testa and 100 seed weight was 95g in the regional yield trials (RYT). “ungsan”showed more resistant to web blotch compared with check variety. In the regional yield trials “ungsan”was outyielded in grain yield to the check variety by 16% with 4.45 MT/ha for grain.

A new peanut variety “harmpyeong”(Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was developed from the cross between the high-yielding cultivar “hindaekwang”and the very short stem cultivar “CGV94216” This Virginia plant typed “harmpyeong”has 33 cm stem height and 18 branch number Each pod with ellipse-shaped large kernel has two grains with brown testa and 100 seed weight was 88 g in the regional yield trials (RYT). This variety also showed more resistant to web blotch compared with check one. Especially it has resistance to lodging until harvesting owing to short stem. In the regional yield trials “harmpyeong”was outyielded than check variety by 13% with 4.35 MT/ha for grain.

간식용 대립 땅콩의 선호도가 높음에 따라 대립 양질 다수성 신품종 육성을 위하여 단경 다수성인 ‘CUP brittle’과 조숙인 ‘대광’를 인공교배하여 육성한 조평의 주요 특성을 요약하면 다음과 같다. 조평은 버지니아 초형으로 개화기가 빠르고 지상부 생장습성은 직립 단경 다분지성이다. 조평은 대광땅콩에 비해 성숙협수, 성숙협비율등 양호한 등숙특성을 보이며 100립중이 87g인 대립품종이다. 도복은 매우 강한 특성을 나타내며 갈반병, 그물무늬병, 흑반병에 비교적 강하고 낙엽율도 낮아 생육 후기까지 녹엽 유지에 유리하였다. 조평은 단백질이 30.9%, 기름함량이 43.1%이고 양질지방산중 올레산과 리놀산의 비율이 2.1로 산화안정성이 양호한 특성을 가진다. 조평은 3년간 지역적응시험 결과 대광땅콩에 비하여 전국평균 8.37ton/ha로서 11% 증수되었다. 조평은 개화가 빠르고 병해에도 강한 편이나 충분한 성숙을 위하여 강원도와 산간 고냉지를 제외한 지역에서 적응성이 높은 품종이다.

간식용 풋땅콩의 선호도가 높음에 따라 대립 양질 다수성 신품종 육성을 위하여 자색종피인 ‘HYQ(CG)S-10’와 다수성인 ‘신광땅콩’을 인공교배하여 육성한 참원의 주요 특성을 요약하면 다음과 같다. 참원은 신풍초형으로 주경과 분지에서 개화 및 착협하고, 지상부 생장습성은 직립 소분지성이다. 참원은 팔광땅콩에 비해 성숙협수는 같으나 100립중이 90g으로 5g 더 높은 대립품종이다. 갈반병, 그물무늬병은 팔광땅콩 보다 강하고 낙엽율도 낮아 생육후기까지 녹엽 유지에 유리하였다. 참원의 기름함량은 25.8%, 단백질이 31.3%로서 팔광땅콩에 비해 높고, 탄닌함량이 낮고 유리당 함량이 높아 풋땅콩의 품질이 양호하다. 참원은 3년간 지역적응시험 결과 대광땅콩에 비하여 풋땅콩이 전국평균 8.93MT/ha로서 18%, 종실이 3.95ton/ha로서 4% 증수하였다. 참원은 개화가 빠르고 병해에도 강한 편이나 충분한 성숙을 위하여 강원도와 산간 고냉지를 제외한 지역에서 적응성이 높은 품종이다.

This experiments were conducted to evaluate suitable plant-type and cultivars for producing fresh pod peanut from late seeding as succeeding crop, compared with early seeding as proceeding crop or single cropping. 12 cultivars according to grain weight and plant types, 6 virginia typed cultivars(ssp. hypogaea) and 6 shinpung typed cultivars(ssp. fastigiata), were used for early and late seedings. The plant growth and yield potential in early seeding were better than those in late seeding. But the ratios of dry/fresh pod and of mature pod in late seeding were higher than those of early seeding. The yield of fresh pod by cultivars in two seeding times showed significant correlation with pod scale such as fresh pod weight, 100-grain weight, and dry seed yield positively, but pod number negatively in early seeding only. Yield of fresh peanut between Virginia and Shinpung types didn't show significant difference in early seeding, but showed in late seeding. Average yield of Virginia typed cultivars showed significantly higher than that of Shinpung typed ones. This yield gap between two plant types was the same tendency on extending seedings to July 20.

This study was conducted to investigate the influence of drought stress during the pod developing and seed filling stage on source-sink relationships of soybean (Glycine max). Drought treatments were imposed by withholding water at the full-pod stage, 19 days after flowering, and then limited watering was relieved at 15 days after the initiation of drought treatment. Soybean seed yield was reduced by 39% mainly due to decreased pod number under drought stress, but the 100-seed weight was relatively less reduced. In spite of the 15-day drought during the full-pod stage, soybean produced good seeds showing similar l00-seed weight, protein, starch and soluble sugar content to those from the well-watered. Although drought during the full-pod stage caused source limitations; i.e. accelerated leaf senescence and reduced leaf soluble sugars, it did not cause limitations of other source characteristics such as SGR and leaf starch level. This is because the reduction in size of sinks, such as pod and seed abortions compensated for source limitations, resulting in balanced source-sink as expressed by LAR and the ratio of leaf area to seed dry weight. Drought stress during the pod developing and seed filling stage did not disrupt the source-sink balance

This study was focuses on the variation of isoflavone contents during seed development and their interaction with major chemical components such as protein, amino acids, saccaharides, lipid and fatty acids. During maturing, lipid, protein, and amino acid contents in soybean seeds showed the highest values at R7 stages, but isoflavone contents were increased until R8 stage. It was noted that malonyl glucosides (64.2~%) are predominant forms among conjugated isoflavones followed by glucosides (30.7~%) , acetyl glucosides (4.1~%) and aglycones (0.9~%) . Sucrose and stachyose were presented as a major saccharide in soybean seeds. As maturing days progressed, they were constantly increased and the highest contents were observed at R8 stage. While small quantities of raffinose, fructose, glucose, maltose, DP3 (DP: degree of polymerization), DP6, and DP7 were detected. These results showed that saccharide composition at the beginning of seed development is primarily monosaccharides with little sucrose and oligosaccharides, but as maturing days proceeds, sucrose and starch increase with concomitant decrease in monosaccharides. Sucrose and stachyose were positively correlated with isoflavone (r=0.780, 0.764 at p<0.01, respectively), while fructose, glucose, maltose, and DP7 were negatively correlated (r=-0.651, -0.653, -0.602, and -0.586 at p<0.05, respectively). Soybeans at R8 stage were high in protein and amino acid, but low in free amino acid contents. Protein and amino acid contents showed positively significant correlations with isoflavone (r=0.571 and 0.599 at p<0.05, respectively), but free amino acid content were negatively correlation with isoflavone (r=-0.673, p<0.01). The lipid content reaches its final content relatively early stage of seed development and remains constant as compared with other chemical components. Among the fatty acids, although varietal difference was presented, stearic acid and linolenic acid were gradually decreased, while oleic and linoleic acid were increased as seed maturing progressed. Lipid was significantly correlated (r=0.754, p<0.01) with isoflavones. However, neither saturated fatty acid nor unsaturated fatty acids significantly affected the isoflavone contents of maturing soybean seeds.

It has been believed that supplementation of human diets with soybean products markedly reduces human cancer mortality rates. Specifically the black colored soybean has been considered as having a medicinal effect since ancient times in Korea. Six genotypes of black colored soybean and a normal variety were investigated for their biological effect. While the isoflavone content of black colored soybean was higher than the normal soybean, saponin content of the seeds was not different among varieties. In the case of antioxidant activity, black colored soybean showed over 3 times increase in the active effect than the control variety. When feeding soybean extract, a general change of alcohol resolving effect, such as alcohol dehydrogenase activity, was observed in the blood and liver of rats.

This study was carried out to evaluate the seed quality of soybeans produced from upland and drained-paddy fields. Soybeans from drained-paddy field showed significantly higher in the 100 seeds weight and greater in the size of seed length than those from upland fields. However, there are no significant differences in seed width and thickness between upland and paddy fields. In case of Hunter's color value, the lightness (L) was significantly higher in the upland soybeans, but the a (redness) and b (yellowness) values were higher in the drained-paddy field soybeans. Seed appearance of drained-paddy field was poor than that of upland field. Soybeans produced from the drained-paddy field showed higher protein content, whereas, lipid and ash contents were higher in the upland field. Soybeans from upland field had lower contents of total amino acids compared to drained-paddy fields. No statistical differences were found in palmitic, linoleic, and linolenic, but stearic, oleic, saturated fatty acids (SFA), and unsaturated fatty acids (USFA) showed significant differences between soybean seeds from upland and drained-paddy fields. Genistein content was higher in the drained-paddy fields, while daidzein and glycitein contents were higher in the upland fields. This result suggested that the soil condition of drained-paddy field is more favorable to synthesis genistein than daidzein and glycitein.

This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

Small seed size is one of the major traits of soybean cultivars for sprouts with regard to high sprout yield. This study was conducted to identify quantitative trait loci (QTL) for seed size and weight in a set of F 6 seeds of 89 lines derived from a cross between 'Pureunkong', a soybean cultivar developed for sprouts and 'Jinpumkong 2', a soybean cultivar with no beany taste in seed due to the lack of lipoxygenases. The genetic map of 25 linkage groups with a total of 98 markers including RFLP, RAPD, SSR and classical markers was constructed from this F/sbu 5/-derived population and was used for QTL analysis. 'Pureunkong' was significantly smaller (P＜0.01) than 'Jinpumkong 2' in seed size and seed weight. Genetic variation was detected and transgressive segregation was common in the population for these traits. Seven DNA markers including opT14-1600 in LG A2, opF02-400 in LG B2, Satt100, opC09-700, opG04-730 and opQll-650 in LG C2, and opY07-1100 ＆ 1000 in LG(unknown) were significantly associated and accounted for 4.7 to 10.9% and 5.1 to 10.1 % of the phenotypic variation in seed size and seed weight, respectively. 'Pureunkong' alleles increased seed size and seed weight at the all four significant marker loci on the LG C2. These marker loci in LG C2 were closely linked and were presumed to be a single QTL. Overall, at least three independent QTLs from 3 linkage groups (A2, B2, and C2) were putatively involved in the control of seed size and seed weight.