In this study, the acute toxicity of Dendropanax morbiferus H.Lév leaf hot-water extracts (DMWE) was examined in male and female ICR mice. Mice were orally administered the DMWE at dose levels of 0, 250, 500, 1,000 and 2,000 mg/kg body weight (BW) for single-dose toxicity test. There were no significant differences in change of BW between control and all DMWE treated-groups. In hematological and blood biochemical analysis, none of the parameters were affected by the DMWE. Similarly, there were no significant effects on markers for liver and kidney functions in all DMWE treated-groups. Since there were no adverse effects of the DMWE in single oral toxicity tests, even at the highest doses, it was concluded that the lethal dose 50 (LD50) of DMWE is estimated at > 2,000 mg/kg BW.

본 연구에서 Nematus tibialis를 국내에서 처음으로 보고한다. 이 종의 사진과 진단, 색 변이, 발육 단계와 분포 등을 제공하였다.

South Korea has a temperate climate with four distinct seasons. However, summers are extremely hot and humid, which negatively affects industrial animal production. Hanwoo are native cattle that have traditionally been raised in the natural environment of Korea. The present study investigated the effects of birth and lactation season on the birth and weaning weights of Hanwoo calves. Data were collected from 100 local breeding farms between 2016 and 2021. A total of 56,970 (males, 29,530; females, 27,440) Hanwoo calves were classified according to sex or birth and weaning season (March–May, spring; June–August, summer; September– November, fall; and December–February, winter). The birth weight of Hanwoo calves differed according to the birth season. As such, birth weight of the summer-born calves was the lowest. Additionally, the 90-day weaning weight was positively correlated with birth weight. Interestingly, however, the 90-day weaning weight was not related to the birth season but was related to the 2-month seasonal effect during the lactation period. Furthermore, the 90-day weaning weight was the lowest during the summer lactation period. In the beef cattle industry, daily weight gain is an important economic characteristic related to feed efficiency and growth. Our findings will contribute the management of Hanwoo cattle and analysis of changes in economic characteristics due to high temperatures.

Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/ drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and antiinflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient’s synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RAhMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

Pseudo-pheochromocytoma is defined as an adrenal tumor that has the typical characteristics of pheochromocytoma likely hypertension but is histopathologically diagnosed to other types of adrenal tumors. 11-year-old, castrated male Maltese presented for a left adrenal mass with hypertension. According to clinical signs and radiological features, a temporary diagnosis was made of pheochromocytoma. The adrenalectomy was performed and the histopathological examination of the resected adrenal gland was diagnosed as cortical adenocarcinoma. This report describes the case of pseudo-pheochromocytoma in a dog and implies the salience that even if pheochromocytoma is diagnosed based on all test results, pseudo-pheochromocytoma should not be excluded.

Influenza A viruses (IAVs) are members of the family Orthomyxoviridae and genus Orthomyxovirus. Avian and mammalian species are the host of IAVs, which includes humans and dogs. Canine influenza virus (CIV) is an emerging pathogen that causes severe and acute respiratory diseases in dogs. This study monitored the antigen and antibody against CIV in dogs in the Republic of Korea (ROK) from 2016 to 2021. One thousand and seventy-two nasal swabs and 1,545 blood samples were collected from animal hospitals and animal shelters. Five nasal swabs in 2017 and seven in 2018 from stray dogs were positive for CIV according to RT-PCR. The prevalence of H3N2 CIV ranged from 9.5% to 24.8%, according to the hemagglutination inhibition (HI) assay. On the other hand, none of the serum samples from 2018 to 2021 showed seropositivity against the avian H5, H7, and H9 viruses. The HI titers for H3N2 ranged from 16 to 512. The distribution of HI titer 16–32 was 57.6% in seropositive samples. The pet dogs were vaccinated against CIV, but the stray and military dogs were unvaccinated. In 2017 and 2018, the seroprevalence of CIV in stray dogs was higher than in the other years, and viral RNA was detected in nasal swabs. It may mean previous exposure of stray dogs to CIV. With the increasing number of pet dogs and the close contact between humans and dogs, canines could serve as an intermediate host for transmitting IAVs to humans. Therefore, continuous surveillance of CIV is needed for public health and the potential emergence of novel zoonotic viruses.

Diabetic encephalopathy is a major complication with cognitive impairment and neurodegeneration in patients with type 1 or type 2 diabetes mellitus (DM). DM-induced glucolipotoxicity is a risk factor for Alzheimer’s disease–like phenotype, including amyloidogenesis, tau hyperphosphorylation, and neuronal apoptosis. Although the detailed mechanism underlying the pathogenesis of diabetic encephalopathy remains unclear, mitochondrial oxidative stress is emerging as a key factor for diabetic complications and neurodegeneration. A deeper understanding of the regulatory mechanism of mitochondrial oxidative stress under hyperglycemic conditions will provide insights into the development of therapeutic strategies for diabetic encephalopathy. Here, we review the role of mitochondrial oxidative stress in diabetic encephalopathy and the regulatory mechanisms by which high glucose induces the generation of mitochondrial reactive species oxygen species in neuronal cells. This review also summarizes the mitochondrial-dependent and -independent pathways (O-linked-N-acetylglucosaminylation, calcium, and glycogen synthase kinase 3β signaling) that regulate mitochondrial oxidative stress in a DM model.

The dimensioning machine installed in the hot cell has been used for 20 years. It has been used for a long time so it was often malfunction due to aging and radiation. In addition, some parts of apparatus were discontinued and there were a lot of problems in maintenance and repair. In the old measuring system, the operator’s subjectivity was much involved. The process of control the focal length (distance between lens and specimen) by operator’s sense is a good example. The existing dimensioning machine was the Kh-7700 of Hirox Co., Ltd. As the equipment had been used for a longtime, additional utilities such as jigs, lighting module and servo motors have been customized and used. The same company’s apparatus was selected for the reasons that it did not need to manufacture a new utility so it could save the cost of radioactive waste disposal for existing utilities and its radiation resistance which has been used for 20 years in radiation environment. Lighting, standing, stage, controllers, cables and so on had been customized to provide remote control in hot cell. The installation was completed in March of this year in hot cell and has been successfully used until now. Through the improvement of dimensioning machine, an auto-focusing and multi-focusing were available. Therefore, they made it possible to produce high quality data and improve the accuracy of data. And this research could be a good example of how hot cell devices can be built and customized to achieve remote control.

As the decommissioning and decontamination (D&D) of nuclear power plants (NPPs) has actively proceeded worldwide, the management of radiation exposure of workers has become more critical. Radioactive aerosol is one of the main causes of worker exposure, contributing to internal exposure by inhalation. It occurs in the process of cutting radioactive metal structures or melting radioactive wastes during D&D, and its distribution varies according to decommissioning strategies and cutting methods. Among the dominant radionuclides in radioactive aerosols, Fe-55 is known to be the most abundant. Fe-55, which decays by electron capture, is classified as a difficult-to-measure (DTM) radionuclide because its emitted X-rays have too low energy to measure directly from outside of the container. Generally, for measuring DTM nuclides, the liquid scintillation counting (LSC) method and the scaling factor (SF) method are used. However, these methods are not suitable for continuous monitoring of the D&D workplace due to the necessity of sampling and additional analysis. The radiation measurement system that can directly measure the radionuclides collected at the aerosol filter could be more useful. In this study, as preliminary research on developing the radioactive aerosol monitoring system, we fabricated a gamma-ray spectrometer based on a NaI (Tl) scintillator and measured the energy spectrum of Fe-55. A beryllium window was applied to the scintillator for X-ray transmission, and the Fe-55 check source was directly attached to the scintillator assuming that the aerosol filter was equipped. 5.9 keV photopeak was clearly observed and the energy resolution was estimated as 44.10%. Also, the simultaneous measurement with Cs-137 was carried out and all the peaks were measured.

Plastic scintillators can be used to find radioactive sources for portal monitoring due to their advantages such as faster decay time, non-hygroscopicity, relatively low manufacturing cost, robustness, and easy processing. However, plastic scintillators have too low density and effective atomic number, and they are not appropriate to be used to identify radionuclides directly. In this study, we devise the radiation sensor using a plastic scintillator with holes filled with bismuth nanoparticles to make up for the limitations of plastic materials. We use MCNP (Monte Carlo N-particle) simulating program to confirm the performance of bismuth nanoparticles in the plastic scintillators. The photoelectric peak is found in the bismuth-loaded plastic scintillator by subtracting the energy spectrum from that of the standard plastic scintillator. The height and diameter of the simulated plastic scintillator are 3 and 5 cm, respectively, and it has 19 holes whose depth and diameter are 2.5 and 0.2 cm, respectively. As a gamma-ray source, Cs-137 which emits 662 keV energy is used. The clear energy peak is observed in the subtracted spectrum, the full width at half maximum (FWHM) and the energy resolution are calculated to evaluate the performance of the proposed radiation sensor. The FWHM of the peak and the energy resolution are 61.18 keV and 9.242% at 662 keV, respectively.

Nuclear power plants decommissioning is planned to be started in middle of the 2020. It is necessary to develop safety evaluation and verification technology during decommissioning to ensure the safety of security monitoring measures and maintenance measures, appropriate emergency plans and preparations for decommissioning, and the use of proven engineering when establishing decommissioning plan. For this purpose, a nuclear power plant decommissioning plan is prepared in several stages before decommissioning. When a lifetime of a nuclear power plant has reached, it needs to be decommissioned and therefore operator company should submit decommissioning plans to the National Safety and Security Commission. And safety analysis should be included in this document and it is explained in chapter 6. According to the NSSC Notice No. 2021-10, it is largely divided into principles and standards, exposure scenarios, dose assessment, residual radioactivity, abnormal events, and risk analysis. When unexpected radiological accident is happened, both public and occupational dose analysis should be conducted. However, research on the former can be found easily on the other hands, research on the latter is not active. In this paper, method of choosing scenarios of accidents during the decommissioning the nuclear power plants is briefly introduced. Accidents during nuclear power plants decommissioning cases in USA is chosen and its risk is evaluated by using risk matrix and ranked by AHP method. During the decommissioning phases, varieties of radioactive waste is expected to be generated such as contaminated concrete and metal. On the other hand, Dry Active Waste (DAW) is generated and its amount is and its amount is 7,353 drums. Characteristic of DAW is highly flammable compared to concrete or metal. Moreover, depending on method of radioactive waste conditioning and type of radioactive nuclides, release rate of the nuclides varies. Thus this type of radioactive waste is critical to fire accidents and such accident can occur extra dose exposure which exceeds the guideline of the regulatory body to workers. Therefore, in this paper, occupational dose exposure during the fire accident is conducted.

The IAEA recommended considerations for exemption regulations of consumer products containing greater amounts of radioactive isotopes than the amounts specified for generic exemption. One of the major considerations is the expected exposure dose should be less than 10 μSv/y and 1 mSv/y for general cases and low probability cases, respectively, in all predictable scenarios. Under this recommendation, many countries evaluated the radiation dose for exposure scenarios of various products in consideration of the national circumstances and, then, established their own specific exemption regulation. In Republic of Korea, the “Regulation on substances excluded from radioactive isotopes” was legislated to specify consumer products excluded from regulation. However, as the usage status and product specifications has changed over time, it is necessary to periodically verify the validity of the regulation criteria in the view of exemption justification. In this study, we developed the use and disposal scenarios in consideration of the domestic use of thorium-containing gas mantle and evaluated radiation dose of each scenario accordingly. The gas mantles are used as a wick for gas lanterns and the maximum activity of natural thorium contained among the currently available gas mantles is 12.5 kBq. Radioactive isotopes in the decay chain of natural thorium can be divided into three groups according to their physical characteristics, and exposure routes suitable for each group were considered in dose calculation. Currently, most gas mantles are installed in camping lanterns. Therefore, we developed use scenarios related to camping. The average number of camping trips and time spent at the campground were set by the data from Korea Tourism Organization. Tent sizes and vehicle specifications were determined by referring to surveys and products in Korea. The used gas mantle is disposed of in a garbage bag for general waste and transported to landfill or incinerator. We determined the amount of gas mantle discarded in landfill and incinerator by the data from Korea Environment Corporation. The exposure time and amount handled by an individual were determined by considering the number of waste collection vehicles, landfills, and incinerators. Although we assumed the maximum activity of the gas mantle for conservative evaluation, the calculated radiation doses for the use and disposal scenarios were below the general requirement (i.e., 10 μSv/y) in all scenarios.

In this study, a manual that can be applied to conflict management of clearance waste recycling by stakeholders was researched to recycle clearance waste that is most frequently generated when decommissioning nuclear power plants. In order to develop a manual that can be applied to conflict management, the content of the conflict should be derived first. In order to derive conflict, it is necessary to organize major issues in recycling clearance waste in consideration of domestic nuclear energy and social environment. In order to organize major issues in consideration of the domestic environment, a literature survey and a domestic current situation investigation were conducted. At this time, the subject of the major issue was selected based on the Level 1 influencing factors of the previous study. As a result of the investigation, it was confirmed that there were many major issues due to lack of reliability/understanding in nuclear energy/radiation. Through this Conflicts caused by recycling clearance waste were derived based on the organized issues. As a result of deriving conflicts, eight conflicts were derived below. 1) Reduced business availability due to lack of understanding/reliability 2) Lack of reliability in the selection and technology of nuclide analysis technology 3) Additional time and equipment required due to establishment of clearance waste regulatory requirements 4) Low economic benefits due to reduction in the effect of substituting raw materials 5) Political interference due to worsening public opinion 6) Rejection of final products due to recycling due to distrust of radiation 7) Public acceptance along the transport route from the source to the recycling plant 8) Business promotion deteriorated due to changes in energy policy As a result of the derived conflict analysis, the most conflicts related to lack of reliability/understanding in nuclear energy/radiation were derived. Accordingly, in future research, it is necessary to prepare a specific plan to enhance the understanding of stakeholders about self-disposal waste recycling. Considering that research that can solve the conflicts that will be faced when the domestic/foreign clearance waste recycling industry is activated is not activated, this study is meaningful in that it derived the conflicts that will be faced when recycling clearance waste. Also, it is expected that the conflicts derived from this study will be used meaningfully in the establishment of the clearance waste recycling management manual.

The decommissioning of Kori Unit 1 is expected to generate a large amount of clearance waste. Disposing of a large amount of clearance waste is economically costly, so a recycling method has emerged. However, clearance waste recycling is expected to cause many conflicts among various stakeholders. In the previous study, possible conflicts were selected in consideration of the domestic environment and major issues. Based on this, this study classifies stakeholders involved in conflicts by group, and suggests ways to enhance understanding by stakeholder and enhance reliability. In this study, stakeholders are classified into four groups that share the same conflicts, and each of the following measures is suggested. 1) Stakeholder Engagement. 2) Common understanding of radiation risks, dialogue between the public/recycling industry/ regulatory agency. 3) Incentives to promote recycling clearance waste. 4) Reliable outlet store for recyclable clearance waste. The above understanding enhancement measures are presented so that a solution to conflict can be smoothly derived when designing a clearance waste-related consultative body composed of interested parties in the future. As a more specific solution, measures to enhance stakeholder trust can be suggested for each understanding enhancement measure. Reliability enhancement measures are also presented so that they can be applied to each stakeholder group, and these are as follows. 1) Write a stakeholder engagement plan, Measures for stakeholder participation in measuring the radioactivity concentration of clearance waste. 2) Active use of easy-to-understand radioactivity comparison data, Expansion of information on environmental radiation dose to public, nuclear/radiation education, Held a tour event at the nuclear power plant decommissioning site, New website for clearance waste information disclosure. 3) Incentives for recycling industries in which the Ministry of Environment or KHNP partially bears the losses that occur when the sales rate is low. Incentives are provided to consumers by including recyclables of clearance waste for Green Card’s green consumption points. 4) Online outlets open for recyclable clearance waste with easy-to-understand radioactivity comparison data. It is expected that if the above-mentioned reliability enhancement measures are used, it will be possible to secure the trust of stakeholders and reduce the gap between stakeholders in the future clearance-related consultative body.

Cellulose-based wastes can be degraded into short-chain organic acids at the cementitious radioactive waste repository. Isosaccharinic acid (ISA), one of the main degradation products, can form the chelate complex with metals and radionuclides, and these complexes have a potential that can accelerate to move the radionuclides to far-field from the repository. This study characterized the amount of generated ISA from typical cellulosic materials in the repository. Two different degradation experiments were conducted under alkaline conditions (saturated with Ca(OH)2 at pH 12.4): i) cellulosic material mixture under an opened condition (partially aerobic), and ii) cellulosic material under an anaerobic condition in a nitrogen-purged glove box. In the first case, three different types of cellulosic materials–paper, cotton, and wood– were mixed at the same ratio, and the experiments were carried out at three different temperatures (20°C, 40°C, and 60°C). It revealed that both the cellulose degradation rate and generated ISA concentration were high at high reaction temperatures, and various soluble degradation products such as formic acid and lactic acid were generated. The cellulose degradation in this work seems to still stay at a peeling-off process. In the second study, each type of cellulosic material was applied in its own batch experiments, and the amount of generated ISA was in the order of paper > wood > cotton. The above two experiments are supposed to be a long-term study until the generated ISA reaches an equilibrium state.