This study was performed to investigate the enhancement of immunomodulatory activities of Lithospermum erythrorhizon by extreme process. The extracts are WE100 (water extract for 24 hours at 100℃), WE80 (water extract for 24 hours at 80℃), EE (70% ethyl alcohol extract for 24 hours at 80℃) and EPE (extreme process for 30 minutes at 25℃, 500 MPa after 70% ethyl alcohol extracts for 3 hours at 40, 50, 60℃). Extraction yield was increased up to 5~10% by extreme process, compare to the normal extraction such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts was showed in the range of 12.68~15.89% at 1.0mg/ml for human lung cell (HEL299). The EPE40 was showed the lowest cytotoxicity 12.68%. The EPE60 extracted by extreme process increased the growth of human B and T cells up to 12.12×104 cells/ml and 14.88×104 cells/ml, respectively and the EPE60 greatly increased the cytokine secretion of both IL-6 and TNF-α. The extracts by extreme process also exhibited higher levels of nitric oxide production from macrophages than the lipopolysaccaharides. It can be concluded that Lithospermum erythrorhizon has immune activities and The extreme process could increase higher immune activities possibly by immunomodulatory compounds.

According to previous reports, antioxidant activities of Codonopsis lanceolata could be increased by a steaming process. This study was performed to improve its antioxidant activity and skin whitening activities of C. lanceolata by high pressure and stepwise steaming complex process. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%, and for phenol and flavonoid contents, complex processed C. lanceolata contained higher than those from conventional extraction process or steaming process alone. The Cytotoxicity of all C. lanceolata extracts also showed low cytotoxicity against human fibroblast cell (CCD-986sk) as 4.49 ~ 10.40%. In whitening activity, high inhibition of tyrosinase activity was estimated as 25.08% by adding the extracts from complex process. We found that whitening and antioxidant activity of complex processed C. lancolata extract was higher than those obtained from conventional extraction and a steaming process because various kinds of antioxidant compounds could be easily released by combined process, compared to one of each process.

This study compared the contents of low molecular ginsenoside according to fermentation process in low grade fresh ginseng. Low grade fresh ginseng was directly inoculated with a 24 h seed culture of Bifidobacterium Longum B6., Lactobacillus casei., and incubated at 36℃ for 72 h. Bifidobacterium Longum B6 was specifically was found to show the best growth on 3,255×106 CFU/ml after 48 h of fermentation. The content of ginsenoside Rb1, Re and Rd were decreased with the fermentation but ginsenoside Rh2 and Rg2 increased after fermentation process. In the case of low molecular ginsenoside conversion yields were 56.07% of Rh2, 12.03% of Rg3 and 77.11% of Rg2, respectively. In addition, compound-K was irregular conversion yield as long as 72 h of fermentation. This results indicate that fermentation process could increase the low molecular ginsenoside in low grade fresh ginseng.

최근 기후변화로 인하여 한반도의 기후는 태풍의 강도가 강해 질 수 있는 조건으로 변화하고 있다. 또한 태풍은 전체 자연 재해 중 적은 빈도에도 불구하고 한번 발생하면 다른 자연 재해보다 큰 피해를 준다. 그러나 태풍이 한반도에 내습하기 전에 정부·지방자치단체·개인의 차원에서 대비를 철저히 한다면, 태풍에 대한 피해를 어느 정도 저감할 수 있다. 따라서 한반도에 내습하는 태풍으로 인한 피해 원인 및 그 규모를 파악하는 것은 매우 중요하다. 태풍이 한반도에 영향을 미치는 기간 동안 재산 피해를 일으킬 수 있는 요소에는 여러 가지가 있지만 그중에서도 순간적으로 발생하는 강한 돌풍은 주택과 건물의 유리창, 지붕, 외장재, 그리고 공공시설 및 거리의 간판 등 구조물을 파괴하여 국민의 안전과 재산을 위협한다. 따라서 본 연구에서는 방재적인 차원에서 태풍 시기 발생가능한 최대풍속 및 그에 따른 피해 규모를 산정하는 ‘한국형 Risk Assessment Model’ 의 방재적인 차원에서 활용 가능성을 검토하였다. 또한 이 모델을 이용하여 과거 한반도에 영향을 미친 태풍 시기에 발생가능한 최대 풍속인 3-second gust를 산정하고 진로에 따른 분포 경향을 살펴보았다. 그리고 이를 이용하여 태풍 내습 시 강풍에 취약한 지점을 선정하였다. 본 연구의 주요결과는 다음과 같다. 첫 번째, 한국형 Risk Assessment Model이 방재적인 측면에서 활용가능한지 검토한 결과 모델에서 모의된 풍속이 실제 풍속의 경향을 잘 따르면서도 약간 크게 모의 되어 방재적인 측면에서 활용하기에 적절하다고 판단되었다. 두 번째, 태풍의 진로 유형별 3-second gust의 분포와 피해액 분포를 살펴본 결과 강풍이 나타나는 분포와 피해가 나타나는 지역이 유사하게 나타났다. 세 번째, 강풍에 의한 피해규모는 태풍의 진로뿐만 아니라 태풍의 진로에도 큰 영향을 받는 것으로 나타났다. 네 번째, 연구 기간 동안 태풍에 의한 강풍 피해에 가장 취약한 곳은 부산·경남 지역으로 나타났다. 이와 같은 결과를 바탕으로 한국형 Risk Assessment Model의 활용 가능성과 활용 가능성에 대해 검토 할 수 있었으며, 추후 태풍이 한반도에 내습할 것으로 예측되었을 때 활용한다면 보다 효과적인 방재대책을 수립할 수 있을 것으로 사료된다.

In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.

In this study, we investigated antioxidant activities and whitening effects of Acer mono sap by encapsulation of nanoparticles. Acer mono sap was through ultra high pressure process and then encapsulated by lecithin. Nano-encapsulated The nanoparticles of Acer mono sap showed highest free radical scavengering effect as 89.7% in adding sample (1.0 mg/ml), compared to sap of non-encapsulation. It was showed strong inhibition effect on melanin production test by Clone M-3 cells as 47.8%. High inhibitory of tyrosinase was also measured as 85.8% by adding lecithin nano-particle of 1.0 mg/ml. The nano-particles also showed 14.8% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. These results indicate that Acer mono sap may be a source of cosmetic agents capable of improving whitening effect and antioxidant activites.

This study was to investigate the effect of fermentation extracts on the concentration of serotonin and melatonin in the serum of the ICR mice. The ICR mice were divided into water control group, lactobacillus fermentation solution including (Lactobacillus paracasei and Bifidobacterium longum B6) control group, positive control group (milk and doxylamine succinate), negative control group (caffein) and the groups treated with the extracts of Berberis koreana bark (WE: water extracts, FE-L.P: fermentation extracts of Lactobacillus paracasei, FE-B.L: fermentation extracts of Bifidobacterium longum B6). After ten-day feeding treatment, the mean concentration of serotonin for water control, WE, FE-L.P and FEB. L group was 134.72, 183.01, 232.09 and 223.78 ng/ml, respectively. The mean concentration for FE-L.P and FE-B.L group were approximately 66% larger than that for water control group. The mean concentration of melatonin for water control, WE, FE-L.P and FE-B.L group was 76.92, 106.66, 157.56 and 141.81pg/ml, respectively. The mean concentration of melatonin for FE-L.P and FE-B.L group were also larger than that for water control group. Our results indicated that the fermentation extracts of Berberis koreana bark have relatively greater potential to induce secretion of serotonin and melatonin. Therefore, the fermentation extracts have antidepressant effect.

This study was performed to enhance anticancer activities of Lithospermum erythrorhizon by eluting high amount of shikonin through ultra high pressure process. Extraction yield was increased up to 5~10% by ultra high pressure process, compare to the normal extraction processes such as water solvent extraction, 70% ethyl alcohol solvent extraction. The cytotoxicity of the extracts (1.0μg/ml) from ultra high pressure process was showed the lowest cytotoxicity 13.4% for human lung cell (HEL299). The anticancer activities showed 80~85% by adding 1.0μg/ml of the extracts from ultra high pressure process in several cancer cell lines such as AGS, Hep3B, MCF-7 and HeLa cells. Among them, MCF-7 cell of the endocrine system was highest inhibited than other cells. The anticancer activities of the extracts from ultra high pressure extraction process showed 10~15%, which was higher than the extracts from normal extraction processes. From HPLC analysis of the extracts, the contents of shikonin in the extracts from ultra high pressure process was 11.42% (w/w), which was 20% higher than others. This results indicate that ultra high pressure process could increase the extraction yield of shikonin and other contents, which resulted in higher anticancer activities.

In this study, whitening activity of Lithospermum erythrorhizon extracts were investigated according to several extraction processes: water extraction at 100℃ (WE100) and 60℃ (WE60), 70% ethyl alcohol extraction (EE) and ultra high pressure extraction (HPE) at 500 MPa for 30 minutes at 60℃. The extracts from ultra high pressure extraction showed the highest tyrosinase inhibition and melanogenesis inhibition activities as 52% and 79.5%, respectively, in adding 1mg/ml than others extraction processes. HPE extracts also showed the strong reducing power as 3.19 that absorbance at 450 mm. The contents of polyphenol in WE100, we measured as 10.1μg/ml in adding 1mg/ml. Extracts have a high total flavonoid contents by HPE as 4.1μg/ml at 1mg/ml. We can conclude that better whitening activity of extracts from high pressure extraction was due to high antioxidant activities which could be extracted by higher polyphenol and flavonoid contents in HPE than others.

This study was performed to investigate the enhancement of cosmeceutical activities of Berberis koreana bark by different extraction processes. The extracts are WE (water extract at 100℃, control), USE (ultrasonification for 1 hours at 60℃ with water), HPE (high pressure for 5 minutes at 60℃ with water) and USE + HPE (ultrasonification process for 1 hours after high pressure for 5 minutes at 60℃ with water), respectively. The cytotoxicity of the extracts was in the range of 24.02~26.94% at 1.0 mg/ml concentration. The USE + HPE showed the lowest cytotoxicity. Compared to the WE, total phenolic and flavonoid contents in the USE + HPE increased to 121.5% and 154.2%. The USE + HPE showed the highest activity at 1.0 mg/ml concentration in 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging, inhibition activity of xanthine oxidase and superoxide dismutase (SOD)-like test, respectively. Tyrosinase inhibition of WE, USE, HPE and USE + HPE at 1.0 mg/ml concentration was measured as 17.72, 19.62, 22.83 and 24.16%, respectively. Hyaluronidase inhibition activities of the USE + HPE were higher than 20.8%~29.5% of the WE. Our results suggested that the extracts from ultrasonification process after high pressure extraction has relatively high cosmeceutical activities, and that the bark of Berberis koreana could be considered as a candidate of new functional cosmetic agents.

The bark of Berberis koreana Palibin was fermented by Bifidobacterium longum B6 and Lactobacillus paracasei at 37℃ for 72 hour, then extracted by water solvent at 100℃ for 180 min. Total pholyphenol and flavonoid contents were improved by fermentation process, compared to conventional water extraction. The barks of B. koreana Palibin extracts by B. longum B6 (FE-B.L.) and by L. paracasei (FE-L.P.) showed 17% and 16% cytotoxicity on human normal cell lines(HEK293) at 1.0 mg/ml of the highest concentration, respectively, which was about 3~5% lower than 20% from normal extracts (NE). DPPH radical scavenging activity of the FE-B.L. and FE-L.P. were about 73% and 75.9% higher than 56.8% of NE. The highest inhibitory potency on xathine oxidase and superoxide dismutase (SOD)-like activities were also measured as 31.9% and 61.9% by adding FE-L.P. of 1.0 mg/ml. On tyrosinase inhibition test, the FE-L.P. showed highest activity as 75.9% at 1.0 mg/ml. Generally, FE-L.P. showed higher antioxidant activities as well as higher tyrosinase inhibition activity, possibly due to high contents of total pholyphenol and flavonoid. In general, fermentation of barks of B. koreana Palibin has relatively better biological activities than normal extracts. Specifically, the extracts fermented by L. paracasei showed higher activities than that from B. longum B6.

This work was to improve antimicrobial activities of horseradish by encapsulated with edible biopolymers such as lecithin and gelatin since it has been difficult to directly use horseradish extracts into foods and food containers due to its strong and undesirable flavors. It was shown that most of the nanoparticles containing the extracts were well formed in round shape with below 400 nm diameter as well as fairly stable and less odd flavors in various pH ranges by measuring zeta potentials. The encapsulation efficiencies of nanoparticles were estimated as 66.6% and 53.4% for lecithin and gelatin, respectively. Minimal Inhibitory Concentration (MIC) of both nanoparticles against G(+), Listeria monocytogenes and G(-), Salmonella typhimurium were also measured as 79 ppm based on AIT concentrations in the extracts, whose activities were about 65% higher than the case of adding crude extract. It was also found that the nanoparticles efficiently penetrated into the cell membrane and started to destruct the cells after 6 hours cultivation under Transmision Electron Microscopy observation. These results prove that the nano-encapsulation of the horseradish extracts can be employed to directly treat into the foods and food containers for antimicrobial purposes with the aids of aerosolization system, by using small amounts of the extracts and having less flavors due to masking effects of nanoparticles.

The objectives of this study were to compare the antioxidant activities by high pressure extraction of Codonopsis lanceolata from different cultivation areas; Hoeng-sung, Jeju island, and China. Total phenolic acid contents of Hoeng-sung, Jeju, China were estimated as 732.11, 640.25, and 584.85 mg QUR/100 g DW, respectively. The flavonoids contents of Hoeng-sung, Jeju, China were measured as 80.37, 76.46, and 74.55 mg QUR/100g DW, respectively. Generally, contents of phenolic acid and flavonoids, HPE was higher than conventional extraction process. Hoeng-sung Codonopsis lanceolata showed 64.33% of DPPH radical scavenging activity (EDA, %) in 3.2 mg/ml of Hoeng-sung Codonopsis lanceolata. The reducing power of Hoeng-sung cultivation area Codonopsis lanceolata also showed the high activity as 3.15. In generally, antioxidant activities of Codonopsis lanceolata were increased by high pressure extraction process. Based on these results, higher contents of flavonoids and total polyphenols were found extracted by high pressure extraction of Codonopsis lanceolata grow in Hoeng-sung area than others.

본 연구는 우리나라 어머니와 유아 간의 일상적 내러티브 대화에서 자녀의 일상적 내러티브를 이끌어내기 위해 사용하는 어머니의 대화방식과 유아의 일상적 내러티브 발달의 관계를 알아보는데 목적이 있다. 연구대상은 만 4,5세 48명의 유아와 그들의 어머니 48명으로 총 48쌍을 대상으로 하였다. 본 연구의 결과 첫째, 어머니의 대화방식은 ‘정교한 방식’과 ‘반복적 방식’으로 나타났으며, 정교한 방식의 요소는 정교화 진술, 정교화 질문, 평가, 길이 이고, 반복적 방식의 요소는 반복 진술, 반복 질문, 문장 완성형 진술 및 질문, 기억자극으로 나타났다. 유아의 개인적 변인에 따른 어머니 대화방식의 차이를 살펴보면 유아의 연령과 성별에 따라 어머니의 대화방식은 차이가 없었다. 둘째, 유아의 일상적 내러티브 발달 수준을 분석한 결과 유아의 성별, 연령에 따라 유아의 일상적 내러티브 발달은 유의미한 차이가 없었다. 셋째, 어머니의 대화방식과 유아의 일상적 내러티브 발달 수준의 관계를 알아본 결과, 정교한 방식은 각 하위요소인 정교화 진술, 정교화 질문, 평가, 길이에서 통계적으로 유의한 것으로 나타났다. 구체적 경향성을 살펴본 결과 어머니의 대화방식 중 정교한 방식에서 정교화 진술, 정교화 질문, 평가, 길이의 요소 모두 유아의 일상적 내러티브 발달 수준이 4수준에 이르기까지는 증가하는 경향을 보이다가 4수준이 넘어서면 점차 감소하는 경향을 보였다.