Purpose: The purpose of this study were to compare the satisfaction in major and work value among the each grade of college nursing students. Methods: This study adopted the descriptive comparative design, Subjects were 225 students from two nursing schools in the Seoul city. The instruments used for this study were a self-report questionnaire. Data were analyzed by descriptive statistics, t-test, one way ANOVA, Duncan post-hoc test, and Pearson correlation coefficient using the SPSS WIN 18.0 program. Results: There were significant differences in major satisfaction and especially, senior students showed the lowest score. There were no significant differences in the work value among the grades. The major satisfaction was correlated significantly with the work value. It showed that higher major satisfaction, more positive work value. Conclusion: Based on the results, major satisfaction had significant correlations with the work value among the students. Which proves that the scores of major satisfaction has its effects on the students’ values of the profession. Therefore, new education system using various learning strategies such as simulation and team-based learning is must needed for each grade in order for students to gain satisfaction in their major, which will improve the nature of future nursing environment and their working values.