This study was carried out to investigate the effects of imported 0.25 ml straw frozen semen on fertilizing capacity after artificial insemination. A total of 57 ewes of Corriedale were inseminated at the Namwon Branch, National Anirnal Breeding Institute. Lambing percentage and twinning percentage were 12.3% and 114. 3%, resepectively. Average weight at birth and weaning of 120 days old were 4.5kg and 21.9 kg, respectively. Gestation period was 152.6 days.

This experiment was carried out to study the behavior of the estrus cycle in sows shortened uterine horns and to see whether the embryos could be recovered nonsurgically. The uteri of sows(n=4) were surgically shortened. It took about 3 hours to surgically remove the middle section of both uterine horns. The lengths of the shortened uterine horns were 18.7 to 29.5cm. After treatment, two sows exhibited natural estrus and the intevals from surgery to estrus were 8 days and 15 days, respectively. But the sows were not successful on synchronization and superovulation with PMSG and PGF. In the resurgery for confirmative examination, the sows had 6 and 7 corpus lutelin in ovaries, respectively. One sow had a small adhesion between the infundibulum and ovary, and the other sow had unilateral uterine obstruction at the sutured position and purulent materials in the uterus.

The objective of this experiment was to study some possibilities to simplify freezing, thawing and transfer procedure of one-step straw method comparing with the conventional methods using bovine embryos. The previous work are also designed to investigate the thawing effect by development stage and its quality using the embryos. Results obtained were summarized as follows: 1. A total of 87 embryos from 14 donor cows were frozen-thawed and an average of frozen embryo/donor was 6.2. 2. The survival rates of morula stage(65.4%) were higher than those of blastocyst stage(57.l%) and vice versa in rate of morphological recovery (80% vs 95.4%). However. no significant difference was denoted between them. 3. In difference between the groups of good quality and poor quality. good quality was resulted in a significantly higher embryo survival rate(75%) and recovery rates(95%) than poor quality(P<0.0l). 4. In effects of non-permeable sugar dilution in added to l.0M glycerol. higher survival rates were orderd in sucrose. lactose, raffinose and xylose. But lactose-raffinose, sucrose-trehalose and xylose in added to 2.OM glycerol. 5. The highest survival rates were obtained by direct plunge into the liquid nitrogen with 3.OM concentration both of glycerol and trehalose. 6. The survival rates in vitro condition of one-step and direct plunge methods(75%-87.5%) were significantly higher than those of multiple steps (21.4-52.6%) in in vitro (P<0.0l). However, the results of single-step were critical in comparing to other steps of final pregnant conformation.

This study was carried out to produce superior dairy cattle by embryo transfer. Seven dairy cows were superovulated with divided injection of FSH 4Omg for 5 days started on day 9 to 14 of the estrus cycle and injection of PGF 45mg on day 4 of FSH injection. Donor cows were flushed to collect embryos on day 7 or 8 of the estrus cycle. Fresh embryos collected were transferred to synchronized dairy recipients or frozen using glycerol 3 step method to he equilibrated. And 35 embryos which were frozen using glycerol 6 step method were imported from U.S.A. After glycerol dilution of frozen embryos was done by reverse density during freezing. frozen-thawed embryos were transferred to synchronized dairy or beef recipients. The results obtained were as follows; 1. Total of 24 embryos were collected from 7 donor cows flushed and transferable embryos were 18 (75.0%). 2. Among 24 embryos. morula, early blastocyst, blastocyst, expanded blastocyst and unfertilized ova were 3 (12.5%), 1 (4.2%), 10 (41.6%), 4 (16.7%) and 6 (25.0%), respectively. 3. Heat inducing rate after 1st and 2nd injections of PGF in Holstein and beef cattle was 83.3% and 71.4% and 62.5% and 69.2%, respectively. 4. Among 56 recipients, 23 head were pregnant (41.1%). The pregnancy rate of fresh embryos was 50.0% (1/2 heads) and the pregnancy rate of frozen embryos which were frozen using glycerol 3 step and using glycerol 6 step imported from U.S.A. was 52.6%(l0/19 heads) and 34.3%(12/35 heads), respectively. 5. The pregnancy rate of blastocyst (60.0%) was higher than that of morula (39.0%), early blastocyst (25.0%) and expanded blastocyst (0%). 6. The pregnancy rate of grade I embryos (52.2%) was higher than that of grade 2 (34.6%) and grade 3 (28.6%). 7. The pregnancy rate according to synchrony of recipient with donor was higher in simultaneous recipient (55.0%) and +l2hrs' (53.8%) than -24hrs' (23.5%), -l2hrs' (20.0%) and +24hrs' (0%).

This study aimed to determine the optimal harvesting time for covered barley to make grain silage, in Honam region of Korea. We harvested six varieties of barley every third day from 24 to 42 days after heading (DAH). The moisture content decreased from 62.4% at 24 DAH to 24% at 42 DAH. The moisture content at 36 DAH was 44.3%; however, moisture content at 39 and 42 DAH was lower than 40%. Yield of covered barley significantly increased from 24 to 42 DAH (p < 0.05). Yield at 36 DAH (557 kg/10a) was not significantly different from that at 39 and 42 DAH (p < 0.05). With respect to the feed value of barley grain silage, the amount of crude fiber and crude ash was different by harvesting time (p < 0.05). However, the amount of crude protein, crude fat, and total digestible nutrients (TDN) from 24 and 42 DAH was not significantly different. The pH of grain silage from 24 to 42 DAH was between 3.8 and 4.2 and it was stable until 36 DAH (p < 0.05). However, the pH of grain silage at 39 and 42 DAH was 5.2 and 5.8, respectively, which was higher than the pH of silage with good fermentation quality. The lactic acid content of barley grain silage from 24 to 42 DAH decreased from 5.5% to 0.5% (p < 0.05). The amount of lactic acid at 36 DAH was higher than that at 39 and 42 DAH (p < 0.05). With respect to moisture content, yield, feed value, and fermentation, the optimal harvesting time for grain silage of covered barley was 36 DAH. This could increase the use efficiency of harvesting machine for barley and reduce the harvesting time gap between whole barley silage and grain silage in Korea. Moreover, using barley grain silage for animal feed could reduce the import of corn.

밀 재배면적 확대를 위해서는 노력절감을 통하여 생산비를 낮추고 농가 소득의 증가와 더불어 수입산 밀과의 경쟁력을 향상시키고자 밀 재배 작업의 단계별 체계적인 기계화로 노력절감 및 기계 이용효율 효과를 분석한 다음과 같다. 1. 밀 재배 작업 중 복토작업이 ha당 683분이 소요되어 작업소요시간이 가장 길었으며, 파종단계인 파종, 시비와 복토작업을 한번에 할 수 있는 동시작업기 이용, 제초제 살포는 승용살포기 이용으로 노력절감을 최소화 할 수 있었다. 2. 밀 파종에서 수확까지의 작업 소요시간은 ha당 부분 기계화 작업인 관행(인력)의 118시간 대비 트랙터부 착용 살포기로 밑거름 시비와 파종+트랙터부착용 배토기로 복토+승용관리기 제초제 살포+트랙터부착용 살포기로 웃거름 살포+콤바인 수확 작업체계(작업체계 I)에서 26시간으로 78% 절감되었으며, 작업체계 I에서 파종단계인 시비, 파종, 복토 작업을 트랙터부착용 동시작업기 활용 작업(작업체계 II)으로 18시간으로 85%가 절감되었다. 3. 밀의 생육, 수량구성요소 등 모두 인력에 비해 기계파종이 짧거나 적어서 수량도 떨어지는 경향을 보였다. 따라서 기계파종 방법에 따라 적합한 파종량 설정에 관한 연구가 요구되어 졌다. 4. 기계화에 따른 기계비용 부담을 감안하여 볼 때 손익분기점은 작업체계 I은 3.7ha, 작업체계 II는 4.2 ha로 판단되었으며, 농가 소득은 ha당 각각 778,110원, 849,930원이 향상되는 효과가 있었다

We carried out the experiment to select the suitable sprout soybean varieties for environment-friendly cultivation in paddy field of southern part area, compares of excess moisture injury degree and yield ability among 29 sprout soybean varieties. Plant growth of sprout soybean was generally low in beginning and recovered after flowering due to rainfall. In paddy field cultivation, number of pod per individual and number of seed per individual were less in difference than upland cultivation, and maturing date was delayed 5-14 days than upland cultivation in most species. When environment-friendly cultivation, pest injury was not caused major problem for the growth during the vegetative period of soybean due to ground spider as natural enemy to insect pest. However, damage of stink bugs showed severe during grain filling period, and Dawonkong, Anpyeongkong, Dachaekong and Wonhwang-kong showed susceptible to sting bug. SMV infection was weak and showed some necrosis symptoms in Sokangkong, but black root rot was not infected at all. Bacterial pustule began to be infected slowly from pod enlargement stage in most species, displayed severe symptoms in Dawonkong, Pungsannamulkong, Seonam-kong and Sobaeknamulkong. The symptoms of pod anthracnose, pod blight and purple spot were greatly appeared after flowering. Disease resistance varieties was Paldokong, Kwangankong, Doremikong, Somyeongkong, Pungsannamulkong, Iksa-namulkong, Seonamkong, Sojinkong, Pureunkong, Bosugkong, Namhaekong and Sorokkong. Lodging index showed 3 in Saebyeolkong, and other species displayed slight lodging in 0-3 degree. 100-seed weight is 9.8-17.2g extent and increased 0.1-3.7g than upland cultivation in most species, but decreased in some species. Government purchase standard, species correspond to small-seed-size namulkong (Sizing screen diameter 4.0-5.6 mm) was Dawonkong, Dachaekong, Bosugkong, Seonamkong, Sokangkong, Hannamkong, Somyeongkong and Wonhwangkong. Species which seed yield was higher than Pungsannamulkong (266kg/10a) were Sorokkong, Hannamkong, Bosugkong and Sowonkong. Considering sprout soybean species, disease endurance, insect resistance, lodging resistance, 100-seed weight, yield ability and excess moisture tolerances synthetically, Seonamkong, Hannamkong, Doremikong, Bosugkong, Pungwonkong, Kwangankong, Sowonkong, Dagikong, Paldokong, Eunhakong and Pungsannamulkong were promising for environment-friendly cultivation in paddy field.

반수체 육성은 육종연한을 단축시키기 위해 유용한 도구이다. 본 연구는 밀 품종 조기 육성을 위한 반수체 육종 시스템을 개발하기 위해 반수체 육성시 식물생장조절제 처리가 배 배양에 미치는 영향에 대해서 조사한 것이다. 밀 이삭의 제웅 작업을 수행하고 옥수수와 원연교잡한 후 종자결실은 2,4-D 및 2,4,5-T 150 mg/L, dicamba 50 mg/L 처리에서 가장 좋았으며, 배형성은 2,4-D 및 2,4,5-T 100 mg/L, dicamba 50

This study was to set the guidelines of soil chemical components in order to assure the safety and quality ofthe panax ginseng from physiological disorder. The disorder symptoms appeared on the leaf with yellow spot, atrophy, yel-low-brown spot, also showed red skin and rough skin of the root. Occurrence type of physiological disorder in cultivatedfield divided into two types:type I ‘such as, yellow spot’ consist of single disorder symptom; type II ‘such as, yellow spotand yellow-brown spot’ consist of two or more different disorder symptoms. The individual contribution of soil properties tothe occurrence type was as follows ; The yellow spot was affected by Na>NO3-N>salinity (EC) in soil. The same results wasobserved in red skin. Atrophy was affected by NO3-N>salinity (EC)>Ca>Mg. Rough skin was affected by P2O5>pH>Organic material > K. It showed positive associated to P2O5, pH and K, but negative associated to organic matter. Simulta-neous occurrence of two different disorder, including cases which yellow spot and yellow-brown spot, those were affected byNO3-N > salinity (EC) > Na > Mg. In the case of atrophy plus yellow-brown spot, those also were affected by in the order :NO3-N > salinity (EC) > Ca > Mg > Na. Red-rough skin was affected in the order : salinity (EC) > NO3-N > K > Na. Soilchemical components appear to be related to occurrence of physiological disorder, particularly in salinity (EC) and NO3-N.The salinity (EC) and NO3-N were negative related to plant growth. In addition, exchangeable cation capacity play criticalroles in attributing to complex occurrence of physiological disorder.

The purpose of this study was to identify soil physical characteristics as guideline for high yield potential inginseng cultivated field which produced 6 years root. Harvest yields of ginseng to be divided by parent rock was in order ofphyllite and red shale 3.1㎏/3.3㎡ > granite and gneiss·schist 3.0㎏ > basalt 2.6㎏ > porphyry 2.2㎏ in upland and forestsoil. Also, with classified by topography, it was in order of foot slope and alluvial fan 3.2㎏/3.3㎡ > valley 3.0㎏ > low hill2.9㎏ > hill, lave flow and dilluvial terrace 2.8㎏ in survey tilth. Class determination of soil texture, it was in order of sandyloam 3.1㎏/3.3㎡ > loam and silt loam 3.0㎏ > clay loam 2.9㎏ > silt clay loam 2.8㎏ in survey tilth. Slope condition offarming land, in case of sloping (2~7%), it was 3.1㎏/3.3㎡ but deep sloping (15~30%) caused decreasing harvest yield. Indrainage classes (excessively, well and moderately well), there was no significantly different in harvest yields. Relationshipbetween harvest yield and soil series, Production sites as yielding 3.0㎏/3.3㎡ were seven sites, also it was contained 14 soilseries. Production sites as yielding 2.5~3.0㎏/3.3㎡ were eleven sites, it was contained 16 soil series. Production sites asyielding 2.0~2.5㎏/3.3㎡ were 10 sites, it was contained 4 soil series.

비닐하우스 내에서 일평균 투광율을 21.2%, 24.8%, 30.3%와 같이 3수준으로, 토양수분을 11.0%, 12.5%, 15.3%, 18.9%와 같이 4수준으로 조절하여 묘삼의 생육 및 수량을 조사한 결과는 다음과 같다. 엽록소 함량은 투광율이 낮은 조건에서 토양수분함량의 감소에 따라 완만히 감소되었으나 투광량이 높은 조건에서 토양수분함량이 낮아지면 엽록소 함량은 현저히 감소되었다.고온장해율은 투광율이 낮은 조건에서 토양수분함량이 11.0%처럼 매우 낮을 때에만 현저히 증가되었지만 투광량이 높은 조건에서는 토양수분함량의 감소에 따라 고온장해율은 급격히 증가되었다. 3.3m2(칸)당 생근중, 주당 생근중 및 사용가능묘삼수는 투광율이 증가되고 토양수분함량이 감소될수록 뚜렷이 감소되었는데, 토양수분함량이 적절한 조건(18.9%)에서는 투광율이 증가되어도 묘삼수량의 감소는 적었으나 토양수분함량이 부족한 조건에서는 투광율이 증가될수록 묘삼수량은 현저히 감소되었다. 적변율은 투광율이 높고 토양수분함량이 많을 때 현저히 증가되었는데, 투광율이 높은 조건에서 토양수분함량이 감소되면 적변율도 차차 감소되었으며, 투광율이 낮은 조건에서 적변율은 토양수분함량에 따라 큰 변화를 보이지 않았다.