Gastrodia elata Blume often has been used for the treatment of headaches, convulsions, hypertension, and neurodegenerative diseases. The main active constituents are gastrodin, 4-hydroxybenzyl alcohol, vanillyl alcohol, 4-hydroxybenzylaldehyde and parishin A, B, C and E. Because Gastrodia elata has also unacceptable off-odor (swine barnyard-like) for food, there is a need to reduce it as well as allow for greater utilization as a functional food materials. In this study, a major off-odor producing substance of Gastrodia elata was fractionated by steam distillation and silica gel column chromatography. The substance was identified as p-cresol(4-methyl phenol) by GC-MS analysis and comparison of the retention time with that of an authentic compound in GC. The content of p-cresol in fermented Gastrodia elata was decreased. A fermented sample of Latobacillus sakei for 2 days was reduced to 54.7%, when compared with a unfermented sample. The five parishin derivatives in Gastrodia elata were identified by HPLC-MS analyses, and a comparison of HPLC retention times with those of authentic compounds. When compared with parishin derivatives of an unfermented Gastrodia elata, those of Gastrodia elata fermented by L. sakei, increased to 18.3% for 2 days. Increases of about 14.0~38.4% of the total phenolic compounds and 57.4~77.3% total flavonoids were found in fermented extracts, by 3 lactic acid bacteria strains. They were compared with 97.1±2.9 μg/g and 40.9±2.0 μg/g in the unfermented control, respectively. The extracts of Gastrodia elata Blume that were fermented by lactic acid bacteria had higher DPPH free radical scavenging activity and FRAP reducing power than the unfermented control.

Firefighting hoods protect the head, face, and neck areas of officials while they perform firefighting services. The purpose of this study is to investigate the head size of Korean firefighting officials in order to establish the dimensions necessary to construct firefighting hoods. A total of 98 male firefighting officials participated in this study and 11 body dimensions, necessary for the construction of firefighting hoods, were measured. The data collected from the firefighting officials were compared to the general adult male data from the Size Korea national anthropometric study. The heights, weights, head circumferences, head heights, and bitragion arcs of the firefighters were significantly larger than those of general adult males, which shows that firefighting officials generally have larger body and head sizes than general adult males. Based on the results of Pearson's correlation coefficients, head circumference and head height were judged to be the important measurements for the construction of the firefighting hoods. Thus, these two measurements were chosen as the basic dimensions of the cross tabulation analysis. As a result, head circumferences of 57.00~60.99cm and head heights of 23.00~25.99 cm were found to be important measurement ranges among the firefighters. This study is expected to be used as the basis for the creation of firefighting hoods that help to ensure the safe rescue activities for firefighting officials.

The aim of this study was to evaluate the effects of microencapsulation on the water absorption, DPPH radical scavenging activity (DRSA), hydroxyl radical scavenging activity (HRSA) and tyrosinase inhibition activity (TIA) in corn silk extracts. The lowest value (0.20) of water absorption index (WAI) and the highest value (95.23%) of water solubility index (WSI) were maltodextrin+cyclodextrin microencapulated corn silk extracts (MD+CD) and cyclodextrin encapulated corn silk extracts (CD), respectively. The 18.60% for DRSA value of control was increased to 89.25% for that of CD. The 16.89% for HRSA value of control was increased to 47.46%, and 7.16% for TIA value of control was increased to 39.35% for that of MD+CD, respectively. The MD+CD would be used for functional food and cosmetics materials as antioxidant and skin whitening agents. All investigated responses between control and treatment were statistically significant (p<0.05).

Contents of water soluble vitamin B6 in a total of 62 agricultural products cultivated in local areas in Korea were analyzed by high performance liquid chromatography with fluorescence detector (HPLC/FLD). To verify the method of vitamin B6 analysis, a quality control chart was formulated with in-house control using a mixture of broccoli and shiitake mushrooms. Among cereals, high content of vitamin B6 measured 234.3~260.3 μg/100 g in dried mung bean and soybean. Vitamin B6 content of non-glutinous and glutinous black rice measured 105.0 μg/100 g and 129.7 μg/100 g, respectively. In vegetables, high content of vitamin B6 were measured in passion fruit (104.3 μg/100 g), gat (55.7~84.3 μg/100 g), gomchwi (31.3~88.0 μg/100 g) and garlic (72.7~98.3 μg/100 g). Among fruits, gold kiwi 'Zespri' and green kiwi 'Hayward' revealed high vitamin B6 content of 116.3 μg/100 g and 78.7 μg/100 g, respectively. In persimmons, daebongsi had high vitamin B6 content (36.0~72.7 μg/100 g) than bansi and sweet persimmon. Vitamin B6 content in dried jujube and persimmon increased more than 86.7 μg/100 g compared to fresh materials. Among specialty crops, green tea powder (64.7~251.0 μg/100 g) and sansuyu (172.3 μg/100 g) revealed high content. Of mushrooms, vitamin B6 content of Sparassis crispa (139.3 μg/100 g) was the highest. Vitamin B6 content information of agricultural products in local areas in Korea collected from this experiment will be used as valuable preliminary data for grasp national nutritional status.

This experiment was carried out to enhance the availability of blackberry. Since it is difficult to use blackberry as a fresh fruit, we investigated the quality characteristics of blackberry powder obtained by various drying methods (freeze drying and hot-air drying at 40~80℃). The L- and b-values of freeze-dried powder was higher than hot-air dried powder. The pH (3.2) was lowest and the acidity (14.4%) was highest in freeze-dried powder. In freeze drying, the brix degree was 65.7 °Bx, but it increased from 54.7 °Bx to 68.5 °Bx with increasing temperature during hot air drying. The total polyphenol and flavonoids contents were the highest in freeze-dried powder, at 9.3 and 6.2 mg/g, respectively. The levels increased as temperature increased in hot air drying. Anthocyanin content in freeze-dried powder was 8.51 mg/g, while it sharply decreased to 1.17~2.45 mg/g in hot-air drying. Vitamin C content in freeze drying (979.4 μg/g) was higher than that in hot-air drying (48.3~303.2 μg/g). The sample concentration required for 50% reduction of DPPH free radical scavenging (RC50) was 79.7 μg/mL in freeze drying, and showed high antioxidant activity. Also it decreased from 122.4 μg/mL to 87.7 μg/mL with temperature increase during hot air drying. We therefore conclude from the above results that freeze drying is more suitable for the production of blackberry powder, because this method showed high value of chromaticity, total polyphenol, flavonoid, anthocyanin content, vitamin C and antioxidant activity.

Rockfish was a commercially important fish specie in marine ranching areas in Korea. To estimate density and biomass of rockfish using acoustic method, target strength (TS) information is required on the species. This study measured TS dependence on tilt angle and size on 14 live rockfish individuals at 38, 70, and, 120 kHz by ex-situ measurement (tethered method) and acoustic scattering model (Krichhoff ray mode, KRM). The swimbladdered angle ranged from 18 to 30˚ (mean ± s.d. = 26 ± 4˚ ). The mean TS for all individuals was highest -35.9 dB of tilt angle -17˚ at 38 kHz, -35.4 dB of tilt angle -25˚ at 70 kHz, and -34.9 dB of tilt angle -22˚ at 120 kHz. The ex-situ TS-total length (TL, cm) relationships were TS38kHz = 20log10(TL) - 67.1, TS70kHz = 20log10(TL) - 68.6, and TS120kHz = 20log10(TL) - 69.9, respectively. The model TS-total length (TL, cm) relationships were TS38kHz = 20log10(TL) - 66.4, TS70kHz = 20log10(TL) - 67.0, TS120kHz = 20log10(TL) - 67.0. The two measurements between the ex-situ TS and KRM model for TS-tilt angle and fish size were found to be significantly correlated.

본 연구는 옥수수수염 추출 후 미세캡슐화 과정에서 피복제의 종류에 따른 생리활성을 대조군과 비교분석하였다. 에탄올 추출 후 Celluclast 1.5L FG 효소처리를 한 시료를 핵물질로 사용하였으며 피복물질로는 말토덱스트린(MD), 사이클로덱스트린(CD), 말토덱스트린과 사이클로덱스트린(MD-CD)을 1:1로 혼합한 세 가지 피복물질을 이용하였다. DPPH radical 활성 측정 결과 대조군은 18.60%이었으며 미세캡슐화 처리군에서는 79.40-89.25%로 나타났고 CD 처리군은 대조군에 비하여 약 4.8배 높은 값을 보였다. Hydrogen peroxide 소거 활성에서는 대조군은 10.03%로 나타났으며 CD 처리군이 30.93%로 가장 높았으며 MD-CD 처리군이 25.64%, MD 처리군이 21.55%로 각각 나타났다. Hydroxyl radical 소거활성 측정 결과 대조군에서는 16.89%이었으며 MD-CD 처리군이 47.64%로 가장 높았으며 CD 처리군과 MD 처리군이 각각 41.89와 37.50% 순으로 나타났다. Elastase 저해활성 측정 결과 대조군의 경우 43.78% 저해활성을 보였으며 MD 처리군이 처리군 중 가장 높은 48.50%로 나타났다. Tyrosinase 저해활성에서는 대조군은 7.16%로 낮은 활성을 보인 반면 MD 처리군이 40.42%로 가장 높았다. 모든 분석 치에서 대조군과 처리군 간에 5%내에서 유의적 차이를 보였다.

본 연구는 옥수수수염 추출 후 미세캡슐화 과정에서 피복제의 종류에 따른 이화학성질을 대조군과 비교분석하였다. 에탄올 추출 후 Celluclast 1.5L FG 효소처리를 한 시료를 핵물질로 사용하였으며 피복물질로는 말토덱스트린(MD), 사이클로덱스트린(CD), 말토덱스트린과 사이클로덱스트린(MD-CD)을 1:1 로 혼합한 세 가지 피복물질을 이용하였다. 주사전자현미경으로 1,800배 확대한 후 표면미세구조를 분석한 결과 대조군의 표면은 구형의 상태로 확인 되었으나 캡슐화 분말의 입자표면구조는 구형이 아닌 다양한 형태로 나타났으며 MD 처리군의 표면미세구조는 거칠게 나타났다. 미세캡슐화 전과 후의 입자분포를 비교하여보면 대조군의 평균 직경인 28.84μm에서 MD, CD, MD-CD 미세캡슐화의 경우 각각 5.67, 3.61, 3.85μm로 작아졌다. 미세캡슐화 분말의 수분흡수지수 및 수분용해지수의 경우 대조군의 수분흡수지수는 0.68로 가장 큰 값을 보였으며 미세캡슐화 시료는 0.20-0.26이었으며 MD-CD 처리군이 0.20으로 가장 작은 값으로 나타났다. 수분용해지수에서는 대조군이 72.23%로 가장 작았으며 CD 처리군이 95.23%로 가장 크게 나타났다. 총 메이신 함량에서는 대조군의 경우 3752.79 mg/100 g이었으며 미세캡슐화 시료에서는 2338.85-3338.20 mg/100 g의 범위이었고 MD-CD 처리군이 3338.20 mg/100 g으로 처리군 중 가장 높은 함량을 보였다.

V-Coupling is commonly used as a mechanical fastener to connect the turbine housing and the bearing housing in a turbocharger assembly. The back plate between the turbine housing and bearing housing would be compressed by tightening torque of the coupling bolt in order to protect the gas leakage at a turbocharger’s operation. This paper presents the numerical and experimental method for the prediction of the mechanical behavior and sealing performance of the coupling system. The test was conducted to verify the finite element model by measuring the circumferential and axial direction strains of V-coupling under turbocharger’s assembly load. Finite element analysis was carried out to obtain the mechanical strains and contact pressures of the coupling. It can be seen that the analysis results are in good agreement with the measured strains from the coupling’s assembly load. And, the pressure distribution of the back plate also presented to identify the sealing performance of the turbocharger’s coupling system.

Generally speaking, classifiers xie(些) and dian(点) only involve the unspecified quantities of objects, and can not be used to describe the objects’ features and properties. However, when used in reality, xie(些) and dian(点) can co-occur with other classifiers by forming the construction like “(yi) xie(些)/dian(点)+X+N”. Since there is a tendency to make the expression alive, vivid and concrete, this new type of construction can be regarded as the result of this tendency. Along with the wide use of this construction, the relation between “(yi) xie(些)/dian(点)” and “X” becomes more and more tight, and starts to fossilized. Especially the classifier “ge (个)”, it can appears relatively free in this structure, and the function of “ge(个)” is to make the structure of “(yi) xie(些)/dian(点)+N” discrete. Besides, in some circumstance, “ge(个)” in the structure of “(yi) xie(些)/dian(点)+ge(个)+N”is mandatory in the usage; sentence would be ungrammatical without “ge(个)”. Moreover, this paper will discuss the effect of this new construction on “N”and the whole sentence. And also will point out the similarities and differences between “(yi) xie(些)+X+N” and “(yi) dian(点)+X+N”.

Research and development activities have been actively conducted at universities, research institutions and research laboratories which belong to corporations. Because of blooming research and development activities in various fields, safety accidents in the laboratories happen consistently. The government office established Act on the establishment of safe laboratory environment to decrease safety accident rates and make environment of laboratories better, and it is effective slowly. However, in the case of laboratory safety management of the laboratory where various research and development activities are carried out. So it is difficult to closely monitor them. Even though safety inspections and periodic inspections is regularly conducted, these are focused on facilities and environment. According to a study of Dea-deok science town safety council, accidents in laboratories of 73% have arisen out of careless actions. Therefore, it is important for researchers to know to potential harmful factors in research. there is necessary to make a system to prevent laboratory accidents. The purpose of this study is that the researchers discovered potential hazard factors in laboratories. For that, foreign laboratory safety management systems has applied to domestic laboratories. Four laboratories in targeted institution has been selected to apply the appropriate personal protection equipments, job safety assessment and standard operating procedures. And I found the limitations of the process according to the excavations harmful factors in the research process. To overcome these limitations, Suggest a laboratory safety management system. This study discovered current laboratory safety system limitations and provides alternatives so that effective safety management can be achieved