The Ag0-containing sorbents synthesized by Na, Al, and Si alkoxides have higher maximum iodine capture capacity and textural properties than zeolite-based Ag0-containing sorbents. However, these sorbents were prepared in the form of granules via a step for cutting cylindrical alcogels. Since asmade sorbents decreased packing density, they must be additionally crushed and then classified into an appropriate size for increasing packing density. The bead formation in the step of sol-gelation could bring about the simplification of sorbent preparation process and an improvement of packing density. In the Na, Al, and Si alkoxides as starting materials, sol solution was hydrophilic and lower density than vegetable oil, which transformed sol droplets to sol-gel beads. Thus, in these precursors, sol droplets, which must be sprayed by single nozzle placed at bottom side of oil column, can rise up through oil column. Acetic acid (HOAc) was used as the catalyst for the hydrolysis of Na alkoxide (TEOS) and gelation of the Na+AlSi-OH alcosol. For obtaining sol-gel beads, experiments were performed by the flowrate change of sol solution and HOAc at different nozzle sizes using soybean oil column of 1 m in length. At a sol/HOAc flowrate ratio of 3.85, some Na+AlSi-OH alcogel beads were obtained. After the Ag/Na ion-exchange, Ag content in Ag+AlSi-OH hydrogel was low due to reaction between Na+ and HOAc during sol-gelation and aging step. The Ag+AlSi-OH hydrogel with high Ag content could be prepared by Na addition. After the solvent exchange and drying at ambient pressure, the bead sorbents had higher Ag0 content and larger pore size than granular sorbents. However, further experiments are needed to increase yield rate in bead sorbent.

The Na, Al, and Si akoxides-based sorbents for iodine capture have higher maximum iodine capture capacity and pore properties than zeolite-based sorbents. However, these sorbents were prepared in the form of granules via a step for cutting cylindrical alcogels. Since as-made sorbents decreased packing density, they must be additionally crushed and then classified into an appropriate size for increasing packing density. The bead formation in the step of sol-gelation could bring about the simplification of sorbent fabrication process and an improvement of packing density. For the formation of gel bead, characteristics such as hydrophilic or hydrophobic property and density of sol solution were investigated to design sol-gelation equipment. The sol-gel bead preparation equipment in the reflection of sol solution characteristics was fabricated through selection of oil for formation of sol bead, solvent for collection of gel bead, and nozzle for spray of sol droplet formation. The continuous or discontinuous formation of sol beads from NaAlSi-OH sol solution were observed according to flow rates of 6 to 8 mL·min−1 and nozzle diameters of 0.4 to 0.8 mm. In the sphericity of sol bead, the best sol beads were obtained from 0.5 mm nozzle without clogging by sol solution in the flow rate range of 6–8·min−1.

충북 청주시에 위치한 충북대학교에서 강수에 따른 습성강하물 중의 TN, TP 및 COD의 농도와 오염부하를 조사하였다 강수사상에 대한 샘플은 1998년에서 2003년까지 채집되었다. 오염물질의 강수량가중평균 농도는 TN이 0.60mg/L, TP가 0.014mg/L, COD는 4.8mg/L이었는데, 이는 각각의 산술평균보다 26, 18, 14% 작게 나타났다. TN, TP 및 COD의 농도는 강수량이 증가함에 따라 감소하는 경향을 나타냈다. 모든 수질항목

Embryonic stem (ES) cells have property of self-renewal and can differentiate into the cells of all three primary germ layers. Recently, many growth factors, alteration of culture condition and gene modifications have been used to differentiate mouse and human ES cells into specific cell types. This study was performed to evaluate the differentiation protocol for human ES cells to the endodermal lineage cells. Human ES cells (Miz-hESl ) were cultured on STO feeder layer mitotically inactivated with mitemycin C, and embryoid bodies (EBs) were formed by suspension culture. Differentiation protocol of EBs consisted of three steps: stage I, culture of EBs for 6 days with ITSFn medium; stage II, culture of stage I cells for 8 days with N2 medium ; stage III, culture of stage II cells for 22 days with N2 medium. mRNA levels of the endodermal lineage differentiation genes were analyzed by semi- quantitative RT-PCR. The Oct-4 expression, a marker of the pluripotent state, was detected in undifferentiated human ES cells but progressively decreased after EBs formation. Differentiating human ES cells expressed marker genes of endodermal differentiation and pancreatic islet cells. GATA4, a-fetoprotein, Glut-2, and Ngn3 were expressed in all stages. However, albumin and insulin were expressed in only stage III cells. The human ES cells can be differentiated into endodermal lineage cells by multiple step culture system using various supplements. We are developing the more effective protocols for guided differentiation of human ES cells.