Two strains one of 29 strains were picked out Hm1-1, and Hm3-10 as parental strains for breeding, such as Hm1-1 was taken 15 point as the highest grade. Because Hm3-10 was wild strain collected form Deogyu mountain. The result of RAPD has shown that 29 strains fell into 6 distinct groups while a wild strain collected from DeogYu Mt. did not belong to any of the groups. The 20 selected monokaryon mycelia were crossed by Hm3-10 × Hm1-1, and then we picked up dikaryotic mycelia which did form clamp connection. We acquired 343 dikaryons from Hm3-10 × Hm1-1 with the mating rates of 85.7%, respectively. Total 343 dikaryon strains were cultivated and then 58 strains with good morphological and cultivation characteristics were selected. The differences of the 58 strains were verified by morphology of fruiting bodies. Finally, we selected 3 new strains (Hm15-3, Hm15-4, Hm17-5). Lastly, in order to develop molecular markers that can identify developed 3 new cultivars, were subjected to the random amplified polymorphic DNA (RAPD) analysis using 3 commercially available random primer sets (OPS-1, OPS-10, and OPL-13). I selected 10 distinct DNA bands from the three RAPD gels which were amplified with OPS-1, OPS-10, or OPL-13 primers. Bands 1, 6, and 7 were unique for Hm1-1 and Hm1-6. Bands 2∼5 and 8∼10 were unique for Hm3-10. The sequences were deposited in GeneBank and were used to design the 15-base primer sets using their 5’- and 3’-ends. The primer sets P1∼P5 and P7 produced DNA bands with corresponding sizes from all H. marmoreus strains and presented no strain specificity. The P6 marker appeared only for Hm1-1 while the P8 marker appeared for most hybrid strains except Hm16-1 and the wild Hm3-10. This is interesting because the P6 marker showed broader specificity than the P8 marker in the identification of strains. The P9 marker appeared on Hm16-1, Hm16-2, Hm17-5, and Hm3-10 and the P10 marker appeared on Hm15-3, Hm15-4, Hm16-1, Hm16-2, and Hm3-10. The hybrid strain Hm15-3 was the only strain that did not contain either the P9 or P10 marker. The resulting DNA markers were applied for the identification of the strains and employed for the molecular breeding of H. marmoreus.