The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.

Subunit vaccines are being developed as a potential therapy for preventing microbial pathogen infection. In this study, the immunogenicity of recombinant Brucella (B.) abortus Fe/Mn superoxide dismutase (rFe/Mn SOD) protein as a subunit vaccine against B. abortus was investigated in BALB/c mice model. Brucella Fe/Mn SOD gene was cloned into a pcold-TF DNA vector. The bacterial recombinant protein was expressed using the Escherichia coli DH5α strain with a size of 82.50 kDa. The western blotting assay showed that rFe/Mn SOD reacted with Brucella-positive serum, indicating the potential immunoreactivity of this recombinant protein. After the second and third vaccinations, the peripheral CD4+ T cell population was increased significantly in the rFe/Mn SOD-immunized mice group compared to the PBS control group. Moreover, immunization of this recombinant protein increased the CD4+ T cell population from the first vaccination to the third vaccination. Meanwhile, the CD8+ T cells were slightly enhanced after the second vaccination compared to the first vaccination and compared to control groups. Fourteen days after the bacterial infection, the splenomegaly and the number of bacteria in the spleen were evaluated. The result showed that both rFe/Mn SOD and positive control RB51 decreased the bacterial replication in the spleen and the splenomegaly compared to control groups. Altogether, these results suggested that rFe/Mn SOD could induce host immunity against B. abortus infection.

Chlorine dioxide (ClO2) has recently emerged as an ideal disinfectant and has shown a wide range of antimicrobial activities in various pathogenic microorganisms. In this study, the virucidal effect of ClO2 at low concentration (0.02 ppm) and higher concentration (0.06 – 0.09 ppm) against Adenovirus and Herpesvirus was evaluated based on the NF T 72-281 and ASTM 1053-11 standard methods at different exposure times. The virus suspension was dried onto the carrier and then exposed to gaseous ClO2 (gClO2) at 22 ± 2∘C. For Adenovirus, exposure at a low concentration of ClO2 at the middle height resulted in the average log10 reduction of 0.95, 2.65, and 5.30 after 1, 3, and 6 h post-exposure (pe), respectively. Moreover, more than 4-log10 reduction was achieved at 4 and 6 h pe with higher concentrations of ClO2. On the other hand, the antiviral activity of gClO2 at the middle height was also effective against Herpesvirus. In particular, at 1 h pe, a less than 4-log10 reduction was observed at all examined concentrations of ClO2, whereas exposure for 3 and 6 h (with low concentration) or 2 h (with higher concentration) inactivated completely viruses attached to the carrier. These results suggested that ClO2 fumigation is a potential alternative method for disinfecting healthcare facilities, high-containment laboratories, and households with a safe concentration for human health.

We investigated the effect of a synthetic complement peptide C3a on the outcome of Brucella abortus 544 infection in a murine macrophage cell line RAW264.7 cell. First, we determined the highest non-cytotoxic concentration of the peptide in the cell line. We also found that the peptide significantly increased the growth of the bacteria at 8 and 24 h. Although the number of bacterial CFU was also elevated at 48 and 72 h, the increases were not significant as compared to controls. We further investigated the effect of C3a peptide on the growth of Brucella by pre-incubating the peptide at various temperatures and found that the effect was reversed at 24 h post-incubation suggesting that incubation of peptide at high temperatures including 65°C or 95°C could inactivate its action. This also could indicate the beneficial effect of high temperature during infection. Although several studies reported the inhibitory effect of different antimicrobial peptides including C3a, the present study preliminarily revealed that it had no positive contribution on the control of B. abortus 544 infection in vitro and indirectly to its receptor, CD88, which belongs to GPCR. Moreover, the encouraged further exploration of the effect of other similar peptides would be performed for the purpose of finding Brucella-host cell interaction for the control of disease progression.

This study aims to investigate the effects of exogenous succinic acid (SCA) on Brucella (B.) abortus infection in macrophage RAW 264.7 cells and ICR mice. Firstly, the in vitro experiment was conducted by MTT cytotoxicity and bacterial internalization assay to evaluate the uptake of B. abortus into macrophage cells. Two non-cytotoxic concentrations of SCA demonstrated attenuated invasion of Brucella into macrophages at 30 and 45 min post- infection (pi). Secondly, ICR mice were treated with SCA and infected with B. abortus. On day-14 pi, spleen and blood serum were collected to evaluate the bacterial burden and total spleen weight as well as the production of cytokine/chemokine, respectively. The results showed that SCA treatment promoted bacterial growth and reduced the total spleen weight in mice. Furthermore, SCA treatment increased the level of IL-10 cytokine in the sera, while dampening the production of MCP-1 chemokine compared to the control. The results of bacterial load in spleen and spleen weight together with cytokine/chemokine production profile in the sera indicated that SCA induced the host anti-inflammatory response which is beneficial for the survival of Brucella. Therefore, these findings suggest that SCA contributed to host immunity against Brucella infection and the emerging potential topic-immunometabolism should be invested for further investigations.

The lack of short baselines, referred to as the short-spacing problem (SSP), is a well-known limitation of the performance of radio interferometers, causing a reduction of the ux detected from source structure on large angular scales. The very large number of antennas operated in the Atacama Large Millimeter/sub-millimeter Array (ALMA) generates situations for which the impact of the SSP takes a complex form, not simply measurable by a single number, such as the maximal recoverable scale. In particular, extended antenna congurations, complemented by a small group of closeby antennas at the centre of the array, may result in a double-humped baseline distribution with a signicant gap between the two groups. In such cases one should adopt as the effective maximal recoverable scale the one associated with the extended array and use only the central array to recover missing ux, as one would do with single dish or ACA (Atacama Compact Array) observations. The impact of the missing baselines can be very important and may easily be underestimated, or even overlooked. The present study uses ALMA archival data of the 29SiO(8-7) line emission of the AGB star W Hydrae for a demonstration. A critical discussion of the reliability of the observations away from the star is presented together with comments of a broader scope. Properties of the circumstellar envelope of W Hya within 15 au from the star, many of which are not mentioned in the published literature, are brie y described and compared with R Doradus, an AGB star having properties very similar to W Hya.

We investigated the cytotoxic potential of three different commercially available absorbent feminine hygiene products and one transdermal patch using direct contact and extract exposure methods. Two different cell lines were used – mouse fibroblast L929 and normal human skin fibroblast CCD-986sk cell lines. The test samples were extracted using three different methods in accordance to International Organization for Standardization (ISO). Viability of cells was analyzed using MTT assay and morphology of the cells were also observed using phase contrast microscopy. Overall, the direct contact method using L929 cells showed that all the test samples had no toxic effect when exposed to extracts for 1 h. For the exposure method, no toxic effect was observed in both L929 and CCD986sk cells incubated with all the test samples regardless of the extraction methods used.

본 연구는 폐쇄형 식물공장에서 시금치 수경재배시 세가지의 인공광이 생육, 광합성 및 품질에 미치는 영향을 규명하기 위해 수행되었다. 세가지 광 처리구는 적색 (660nm), 청색 (450nm) 및 녹색 (550nm) LED를 사용하여, R660 / B450 = 4/1 (RBL), R660 / B450 / G550 = 5/2/3 (WWL); R660 / B450 / G550 = 1/1/1 (WL) 비율로 혼합하였고, 동일한 광도로 설정하였다 (PPFD = 190 μmol‧m-2 ‧s-1). 생육조사결과 초장, 엽수는 WL이 가장 적었다. SPAD, 순광합성율, Fv/Fm, LAI, 근권부 생육은 RBL이 가장 높았고 통계적으로 유의하였다. 줄기, 잎, 뿌리의 생체중, 뿌리의 건물중은 세가지 처리구에서 유의한 차이를 보였다. 대조적으로 WL의 칼륨의 함량은 WWL과 RBL 가운데 가장 높았지만, 반면 칼륨과 철의 함량은 RBL이 가장 높았다. 비타민C 함량도 시험구간 유의한 차이를 보였다. 질소와 옥살산 함량은 WL이 가장 높았고, 용해성 고체와 비타민C 함량은 RBL이 가장 높았다. 옥살산, 질소 함량은 WWL에서 감소하는 경향을 보였고, RBL의 옥살산 함량은 WL와 WWL과 차이가 없었다. 모든 처리구에서 Salmonella, E.coli.는 감염되지 않았다. 결론적으로, RBL이 시금치의 생육에 적합하지만, 적색, 청색과 적정하게 혼합된 녹색광은 시금치의 생육에 긍정적인 영향을 미친다고 판단된다.

본 연구는 베트남 하노이 지역에서 8가지 한국 토마토 품종 (핑크탑, 큐피랑, 슈퍼도태랑, 선레드, 선글러브, TP-7 플러스, 러블리 250, 광복)과 1가지 베트남 품종(FM 120)을 대상으로 봄-여름 및 가을-겨울 작기에 따른 수확량 및 과실 특성을 비교 분석하였으며, 그 결과는 다음과 같다. 1. 작기에 따라 초장, 경장, 주당 과실수 및 수확량(kg)은 유의성이 있었으며 가을-겨울 작기에서 높게 나타났다. 전체적으로 한국품종이 베트남 품종에 비해 높은 생육특성을 보인 반면, 과실수와 수확량에서는 베트남 품종이 우수하였다. 2. 토마토 과실의 주요 특성인 과장, 과폭 및 과중은 작기에 따라 유의한 차이를 보였으며 가을-겨울 작기에서 높은 수치를 나타내었지만 TSS 함량은 봄-여름 작기에서 높았다. 건물중과 비타민C 함량은 작기에 따라 유의한 차이가 없었다. 품종별 과실 특성은 한국 품종이 우수하였지만 비타민C 함량은 베트남 품종이 높았다. 3. 봄-여름 작기에서 한국품종의 경우 수확량 및 과실특성에서 가을-겨울 작기 재배에 비해 큰 감소를 보인 반면 베트남 품종은 큰 차이를 보이지 않았다. 따라서 본 실험에 이용된 8가지 한국 품종의 경우 봄-여름 작기보다는 가을-겨울 작기 재배가 적합할 것으로 판단된다.

Tortricinae is the one of three subfamilies (Tortricinae, Olethreutinae and Chlidanotinae) in the Tortricidae and widely distributed around the world. It has been divided into 11 tribes (Horak and brown, 1991). The subfamily can be distinguished from other subfamilies by the two rings of scale on each antenna segment, and the caulis articulated with the juxta by a short membrane. A total of 1010 named species are presenting in Palaearctic region (Razowski, 2008) including 382 species of six tribes are distributed in Europe (Razowski, 2003), more than 260 species were recorded from South East Asia (Robinson, Tuck and Shaffer, 1994). The fauna of Tortricinae in Vietnam is poorly known even some expeditions were previously done by some foreign entomologist. For Vietnamese entomologist, the first investigation on insect pest of agricultural crops was done by National Institute of Plant Protection (NIPP) in the period 1977-1978 and 1997-1998 with only seven Tortricidae species were recorded. In the year, 2000 Kuznetzov annotated a list of 208 tortricid species from Vietnam. Recently, more than 80 species of 31 genera belong to Tortricinae are announced from 2008 to 2009 by Razowski. Of them, 33 species were described as new to science from Vietnam. At present, the tortricid fauna of Vietnam is estimated approximately 315 species (Svetlana V. Nedoshivina, 2010). In this study we briefly reviewed of subfamily Tortricinae in Northern part of Vietnam. Totally, 28 species of 13 genera are recognized, but only 9 species were previously recorded. Besides, Some unidentified species are waiting for further study. The materials for identification are based on the collection of University of Incheon, which were collected from 2004-2010 in the Northern part of Vietnam by Vietnamese and Korean entomologist.

Anthocyanins are naturally occuring phytochemicals and the main components of the coloring of plants, flowers and fruits. They are known to elicit antioxidative, anti-inflammatory and cancer preventive activity. In this study, we investigated anthocyanins in black / yellow soybean seedcoats using different methods of detection - thin layer chromatography (TLC), capillary zone electrophoresis (CZE) and HPLC analysis. The anthocyanins in soybean seedcoats were extracted by five independent methods of extraction and the aglycons (anthocyanidins) of the corresponding anthocyanins were prepared by acid mediated hydrolysis. The anthocyanin / anthocyanidin in black soybean seedcoat showed characteristic TLC mobility, CZE electrophoretic retention and HPLC migration time while little of anthocyanins were detected from yellow soybean seedcoat. The extracted anthocyanins showed pH dependent retention time in CZE and spectral change in UV-Vis spectrum. HPLC analysis of the hydrolyzed extract of black soybean seedcoat identified the presence of four anthocyanidins. The major anthocyanin in black soybean seedcoat was cyanin (cyanidin-3-O-glucoside), with the relative order of anthocyanidin in cyanidin > delphinidin > petunidin > pelargonidin.

The tribe Eucosmini is the second largest tribe of Olethreutinae with more than 1,000 species in over 125 genera, distributed mostly in the Holarctic region, and over half of the known species occur in the Nearctic region (Horak and Brown, 1991b). Enarmonia Hübner, Ancylis Hübner, and its allied genera are tentatively retained in Eucosmini, although they have been treated as the subtribe Enarmoniina (Razowski, 1989) belong to Eucosmini maybe distinguished from other tribes by a combination of the following characters proposed by Kuznetsov and Stekolnikov (1984): 1) one-branched protuberance of the valve; 2) gnathos and uncus very weakened or reduced. In Vietnam, in the period 1977-1978 and 1997-1998, National Institute of Plant Protection (NIPP) recorded only seven Tortricidae species. But none of them are Eucosmini. Recently, Kuznetzov (2001) recorded four species in the South Vietnam: Eucosmogastra aeolantha (Meyrick, 1914), Noduliferola pleurogramma (Clarke, 1976), N. atriplaga (Clarke, 1976), N. phaeostropha (Clarke, 1976). Razowski recorded 14 species of 10 genera of Eucosmini (2009). In this study, we are briefly reviewed of tribe Eucosmini in North Vietnam. As the result of this study, totally 19 Eucosmini species of 13 genera are recognized. Of them, 17 species are reported for the first time from Vietnam such as: Coenobiodes acceptana Kuznetzov, 1973; Epiblema foenella (Linnaeus, 1785); Eucosma calligrapha Meyrick, 1912, etc. The materials identification based on the collection of University of Incheon, which has collected from 2004-2009 in the North Vietnam by Vietnamese and Korean entomologist.

Leaf roller moths Olethreutinae is the second largest subfamily in Tortricidae, comprising worldwide more than 4,300 species (Horak, 2006). Of which, 465 species of 88 genera in South Asia (Diaknoff, 1973). In Vietnam, when studying on fauna of the agricultural pests, National Institute of Plant Protection (NIPP) recorded only 4 Tortricidae species (1977-1978), and 3 Tortricidae species (1997- 1998). From 1996 to 2001. Kuznetzov reported two new genera Fibuloides gen. nov., Pammenpsis gen. nov., and 19 species of Olethreutinae. He also has created a list of 208 Tortricidae species in the North Vietnam. Recently, Razowski recorded 33 species, 11 genera (2008), he (2009) also reported 107 species, 61 genera, of which 61 species of 41 genera belong to Olethreutinae in the North Vietnam. Of which, 25 species are described as new to science, 13 species are new to Vietnam. In the present study, we are briefly reviewed of subfamily Olethreutinae in North Vietnam. We recognized 105 species of 51 genera. Of them, 50 species are reported for the first time from North Vietnam such as: Andrioplecta pulverula Meyrick, 1912; Cryptophlebia repletana Walker, 1863; Dactylioglypha tonica (Meyrick, 1909); Lobesia aelopa Meyrick, 1997; Lobesia ambigua Diaknoff, 1954, etc. The materials identification base on the collection of University of Incheon, which has collected in North Vietnam from 2004-2009.

Independence is a factor which has a significant impact on auditing quality, especially in the recent times where the marketplace is increasingly demanding transparency. The independence of auditors is assessed based on the main criteria such as the reliability of the audit opinions, the accuracy, truthfulness of financial reports, and the objectivity in the opinions of the auditor. The study investigates the effects of non-audit services (NAS) on auditor independence in emerging economies such as Vietnam. Non-audit services include taxation services, management advisory services and outsourcing internal audit services. Based on previously relevant researches, the authors develop hypotheses and a regression model about non-audit services (independent variables) and audit independence (dependent variable). The research collects data by 5-scale Likert questionnaire. The findings indicate the relationship between NAS and auditor independence. Specifically, the results reveal two main factors that have influence on auditor independence, namely, taxation services and management advisory services. The results of our research can be considered as new findings and can be compared to previous researches in Vietnam, which establishes a new viewpoint about the relationship between the NAS and the independence of the auditor. This result can be implemented for countries or jurisdictions which are similar to Vietnam.

The research purpose of the article is to evaluate the effects of the internal control components on the effectiveness of internal control of transport construction enterprises in Vietnam, on the basis of which we made recommendations to contribute to improving the effectiveness of internal control. Sample was collected from Vietnamese medium and large transport construction enterprises, the total number of sample questionnaires sent was 400, 330 filled-up questionnaires was collected and 250 was used for the study purpose. Quantitative research method was used for the study with the help of the SPSS software. The variables in the model are as follows: the dependent variable is the effectiveness of internal control, 5 independent variables are: control environment, risk assessment, control activities, communication, and monitoring. The results of the analysis explain 80.4% variation as a result of the effect of internal control on the Vietnamese transport construction enterprise. All five components positively affect the effectiveness of internal controls in the order of influence, which are: control activities, control environment, communication, risk assessment and monitoring. This study contributes in providing more empirical evidence about the effects of internal control components on the effectiveness of internal control of transport construction enterprises in Vietnam. The study has proposed a number of recommendations to help transport construction enterprises improve control activities and contribute towards improving the effectiveness of internal control.