Seed proteins of allogamous buckwheat (Fagopyrum esculentum Moench. cv. Miyazakizairai) and autogamous buck-wheat were separated by two-dimensional gel electrophoresis (2-DE) and characterized by gasphase sequencing. Total of 100 pro-could be used as mark

The isolation of female gametes is precondition for the satisfactory micromanipulation of gametes in common buckwheat (Fagopyrum esculentum Moench.). Embryo sacs were isolated at flowering stage after brief treatment with minimal concentrations of cell-wa