본 연구의 목적은 의미 있는 대상의 선호도 판단에 영향을 미치는 것으로 알려진 원리인 내적 편향 원리와 규범적 관점 간의 상호작용 양상을 연구하는 데에 있다. 개별 개체의 친숙도가 규범적 관점에 영향을 미친다는 점 또한 고려 하여 친숙도 수준에 따른 두 선호도 원리의 작용 양상을 검증하고자 하였다. 이를 위하여 이중 대안 선택 과제를 활용한 행동 반응 실험을 진행하였으며, 친숙도 수준(높음/낮음)에 따라 각 8개의 단일 개체를 실험 재료로 하여 내 적 편향 원리 준수 여부와 규범적 관점 준수 여부를 조작하였다. 실험 결과, 내적 편향 원리를 준수한 상태에서 개체 가 제시된 경우가 내적 편향 원리가 위배된 상태로 개체가 제시된 경우보다 더 선호되었다. 선호 판단에 걸리는 반응 시간 또한 더 짧은 것으로 나타났다. 그러나 규범적 관점 준수 여부는 선택 빈도 및 반응 시간에 영향을 미치지 않는 것으로 나타났다. 더불어, 개체의 친숙도는 선호도 판단과 관련된 의사 결정에 간섭 요인으로 작용하는 것으로 나타났다. 이와 같은 결과는 개체 선호도 원리 중 내적 편향의 강도가 규범적 관점에 비해 더 우세하며, 단일 개체가 제시될 때 그에 대한 선호 판단에 있어서 그 개체가 가진 속성보다 시각적 문맥과의 상호작용이 중요하게 작용한다 는 것을 시사한다.

환경변화 중요성과 유전적 안전성은 최근에 증가추세의 형질전환 작물에 인식되고 있다. 본 실험은 건조저항성형질전환체 작물의 유전적인 안전성과 환경변이에 따른 농업적인 특성을 분석하였다. 내건성 형질전환체인CaMsrB2-8, 23 과 모품종인 ‘일미’ 및 일반품종을 대조구로GM필드에서 작물학적 생육특성을 조사하였다. 농업적인생육 특성에서 CaMsrB2-8, 23 계통은 모품종인 ‘일미’와년차간, 지역별 평균으로 표현형은 유사하였다. 수량에서 년도별, 지역별 차이는 있지만 통계적인 유의성은 없었다.현미의 미립특성에서 CaMsrB2-8, 23은 모품종인 ‘일미’와차이를 보이지 않았다. 현미의 화학적 성분 분석에서CaMsrB2- 2 계통의 전분과 단백질함량은 모품종인 ‘일미’보다 일반품종인 ‘일품’ 화학적 성분함량이 유사하였다.본 실험결과에서 내건성 형질전환체인 CaMsrB2-8, 23는GM 작물의 후대에서도 유전적인 안전성과 함께 수행될수 있을 것으로 사료된다.

The aim of this study was to investigate the characteristics of paprika dried by various methods. Moisture content of dried paprika was higher in vacuum freeze-dried red paprika (DRP) (11.85%) than in vacuum freeze-drying of osmotic dried red paprika in sugar (RPS), vacuum freeze-drying of osmotic dried red paprika in fructose (RPF) and vacuum freeze-drying of osmotic dried red paprika in corn syrup (RPCS). Carbohydrate content of DRP was the lowest among the dried groups, but fat, protein, and ash contents were the highest in DRP. The pH of paprika was the highest in RPF (5.34), while it was the lowest in DRP (5.05). Reducing sugar and sugar contents of RPF were 28.59 g/100 g and 5.03 °Brix, respectively, which are the highest level among the groups. All color values in inside of paprika were the highest in RPCS, while in outside of paprika, L value is the highest in RPCS, and the value of a, b were the highest in RPS. Regarding the texture characteristics of paprika, strongness, hardness, adhesiveness, chewiness and brittleness were the highest in RPS (p<0.05).

The purpose of study was to evaluate quality, physicochemical and sensory characteristics of acorn Sulgidduk by addition of acorn powder. Acorn Sulgidduk was processed rice powder which added 0, 5, 10, 15, and 20% of acorn powder. The moisture, crude lipid, and crude ash contents of Sulgidduk added with acorn powder was increased by addition amount of acorn powder, but carbohydrate and crude protein contents were decreased. By addition amount of acorn powder, soluble protein content of Sulgidduk were increased, but reducing sugar content were decreased. The L values of Sulgidduk were decreased by addition amount of acorn powder, but increased the a and b values. The hardness, adhesiveness, cohesiveness, springness and chewiness were decreased by addition amount of acorn powder. The sensory test of Sulgidduk added 10% of acorn powder showed the best score in overall preference. Therefore, these results suggested that Sulgidduk added 10% of acorn powder could be applied for produce of acorn Sulgidduk.

도토리를 이용하여 도토리 차의 제조방법을 개발하고 개발된 도토리 차의 이화학적 및 품질특성을 평가하였다. 도토리 차는 네 가지 제조방법으로 제조하여 그 특서의 변화를 조사하였다. 제조방법을 달리한 도토리 차의 일반 성분을 분석한 결과 수분함량은 13.48~39.77%로 볶은 도토 리 차(RAT)에서 가장 낮았고, 3회 증제한 도토리 차(TSAT) 에서 수분함량이 가장 높았다. 탄수화물 및 조지방, 조회분 함량은 볶은 도토리 차(RAT)에서 가장 높았고, 조단백질 함량은 도토리(NA)에서 가장 높았다. 수용성 단백질 함량 은 도토리 보다 증제하거나 볶은 도토리 차에서 높았고, 3회 증제한 도토리 차(TSAT)에서 12.74 mg/g로 가장 높은 함량을 보였다. 환원당 함량 결과 도토리(NA)에서 64.3 mg/g로 가장 높은 함량을 보였다. 색도 측정 결과 L값은 도토리(NA)에서 가장 높게 나타났으며, 도토리를 증제하 거나 볶음에 따라 L값은 감소함을 보였고, a, b값은 도토리 (NA)에서 높았다. 총 폴리페놀 및 플라보노이드 함량은 3회 증제한 도토리 차(TSAT)에서 각 41.15 mg/g, 2.78 mg/g 으로 가장 높은 함량을 보였다. 관능검사 결과 색은 볶은 도토리 차(RAT)에서 가장 높게 평가되었고, 맛과 향은 3회 증제한 도토리 차(TSAT)에서 가장 높게 평가되었다. 떫은 맛은 도토리(NA)에서 가장 떫고, 볶은 도토리 차(RAT)에서 떫은 정도가 가장 낮은 것으로 평가되었다. 전반적인 기호 도에서는 3회 증제한 도토리 차(TSAT)에서 5.42로 가장 높게 평가되었으며, OSAT, RAT, NA순으로 평가되었다. 따라서 본 실험의 결과를 바탕으로 도토리를 이용한 도토리 차의 제조방법을 확립하고 도토리 차의 이화학적 및 품질특 성을 통하여 다양한 식품소재 및 식품개발에 활용할 수 있을 것이다.

본 연구는 산지에 자생하는 참취와 밭에서 재배하는 참취의 품질과 기능성을 평가하기 위하여 에탄올 추출물의 항산화 물질 및 항산화성에 대하여 조사하였다. 참취의 에탄올 추출물의 수율은 블랜칭 건조한 시료에서 높았고, 폴리페놀 함량은 야생 및 재배 블랜칭 건조한 시료에서 각각 35.59, 26.83 mg/g으로 야생 조에서 높은 함량을 보였다. 전자공여능은 농도가 증가함에 따라 모든 군에서 활성이 증가했고 블랜칭 건조가 가장 높은 전자공여능을 보였다. SOD 유사활성능은 야생 참취는 생채에서 높은 활성을 보였고, 재배 참취는 블랜칭 건조에서 높았다. 아질산염 소거능 pH 1.2에서는 생채와 재배 추출물의 농도가 높을수록 높은 소거능을 보였고, 재배보다 생채 참취 추출물이 더 높았다. Xanthine oxidase 저해효과, tyrosinase 저해효과 측정 결과에서는 추출물의 농도가 증가할수록 저해효과가 높았으며 야생 참취에서 높은 저해활성을 보였다. 환원력은 추출물의 농도가 증가함에 따라 환원력은 증가함을 보였고 블랜칭 건조가 높은 환원력을 보였으며 야생 참취 추출물에서 높은 환원력을 나타내었다. 이상의 결과에서 참취 에탄올 추출물의 항산화 활성은 재배 참취의 추출물에 비해 야생 참취의 추출물에서 높았다. 참취 추출물의 항산화 활성은 생채 및 블랜칭 건조 참취 추출물의 항산화 활성이 자연건조 참취 추출물에 비해 전반적으로 높았다. 따라서 참취는 영양성과 항산화 활성이 우수하여 항산화 및 노화 예방에 우수한 식품이며, 참취를 이용한 다양한 가공식품의 개발은 국민 건강과 더불어 참취의 소비확대 및 부가가치 창출에 이바지할 수 있을 것으로 기대된다.

Improving rice high-quality potential is to suffice the food demand of the rapid decreasing consumption, and is a major breeding target recently. We calculated the alkali digestion value (ADV), used to indirectly measure gelatinization temperature, to evaluate the quality of cooked rice in 2013 and 2014. The ADV score of frequency distribution was higher milled rice than brown rice. In total, nine different quantitative trait loci (QTLs) were found on chromosomes1, 3, 5, 6 and 8 in 2013 and 2014. Also, chromosome 5, 8 were detected over two years. The polymorphism using RM223, RM3530, and RM18130 markers can be used to select lines that have a good trait for breeding of high-quality rice. We conclude that selected molecular markers from this QTL analysis could be exploited in future rice quality.

In total, 120 ‘Cheonghcheong/Nagdong’ doubled haploid (CNDH) populations was developed by F1 derived from a crossing whitebacked planthopper (WBPH, Sogatella furcifera) resistance ‘Cheongcheong’ and susceptible ‘Nagdong’ lines. The main objective of this research was to determine the rice resistance optimum screening after infesting by WBPH and identify quantitative trait loci (QTLs) associated with rice resistance in order to provide consistent information for marker-assisted selection (MAS) and develop new varieties. The genetic map with average 9.6 centimorgans (cM) between markers was constructed from 120 CNDH populations using 217 SSR markers. In this study, The result of determine rice with WBPH infestation showed that the rice damage and resistance at 7, 14, and 21 days, were 100%, 76%, and 10% resistance lines of 120 CNDH population. Four QTLs were detected on four regions of the chromosomes 1 and chromosome 8, which contained qWBPH1 and qWBPH8 for resistance rice. The markers were found to be contained in identification the genetic markers RM3482, RM1196, RM3709, RM11694, RM11669, RM17699 and RM264 for marker assisted selection. These markers efficiently were shown to be very useful for MAS in breeding populations of crossing lines associated simple sequence repeat (SSR) marker with WBPH resistance in 120 CNDH populations.

The objectives of this study were to investigate the diversity of natural products (DNP7, 9) in responding to Whitebacked planthopper (WBPH) feeding. Resistant rice (cv. Cheongcheong ), susceptible rice (cv. Nagdong) and susceptible control rice (cv. TN1) were used as materials for WBPH infestation in seedling stage. The treatment was conducted by spraying DNP 7 and 9 for 100 ppm to materials before being fed to 2nd and 3rd instar WBPH while control group was not sprayed DNP 7 and 9. The density of WBPH was 7 insect per plant. As a result, WBPH survival rate of 57% was found in the DNP 7 treatment, whereas those in DNP 9 and control were 27% and 71%, respectively. Resistance score of Cheongcheong, Nagdong, and TN1 in DNP 7 treatment were 3.4±0.8, 5.9±1.9, and 6.8±1.6, respectively, while those in DNP 9 treatment were 1.6±0.8, 4.7±1.6, and 7.9 ±1.4, respectively. The plant heights of Cheongcheong, Nagdong, and TN1 in DNP 7 treatment after 3 week infestation were 19.7±3.0, 23.4±7.5, and 15.8±8, respectively while those in DNP 9 treatment were 32.4±4, 26.3±12.7, and 25.9±8.5, respectively. Moreover, chlorophyll content was examined 3 week post infestation. In both DNP 7 and DNP 9 treatment, the chlorophyll levels of Cheongcheong and Nagdong in were higher than that in control. Based on observation and bio-scoring, plant with DNP 9 was strongly resistant to WBPH feeding and the survival rate of WHPB was lower than plant with DNP7.

The whitebacked planthopper(WBPH), Sogatella furcifera is a serious pest of rice. The nymphs and adults suck phloem sap which causes reduced plant vigor, stunting, yellowing of leaves, delayed tillering in rice. This study was conducted to identify the optimum screening time for improved WBPH-associated QTL analysis and to develop the markers for use in breeding WBPH resistance. Resistance after 7 days infestation was observed in 100 lines(83.3%), after infestation for 14 days, resistance was observed in 14 lines(11.7%), and after infestation for 21 days, resistance was observed in 10 lines(8.3%). However, no after 14 days infestation was as similar as normal distribution in WBPH resistance. QTLs associated of the resistance detected in four regions on qWBPH1 and qWBPH8 in the intervals marker. After 7 days of infestation, the qWBPH1 was located in the interval RM3482-RM11966 and RM3709-RM11694 with LOD 4.0 and RM3709-RM11694 with LOD 3.5. After 14 days of infestation, The qWBPH1 was located in the interval RM3709-RM11694 with LOD 3.3. and RM3709-RM11694 with LOD 3.3. After 21 days of infestation, The qWBPH8 was located in the interval RM17699 with LOD 3.3. The QTLs on chromosome 1 was the most effective RM11694-RM11669 (LOD 3.3, variance 30%). The resistance lines were collected 10 plants of phenotye variation with genotype. The ratios of coincidence were used to determine resistance in 10 plants with phenotypic variation and a genotype of 8 markers. 3 markers were used: RM3482 on chr.1 represented 100%, RM8235 and RM11694 represented 80%, 90%, respectively, RM17699 on chr.8 represented 80% of the coincident ratio. These selected markers will be useful to rice breeding programs interested in new sources of WBPH resistance

The seed shattering played a key role in the crucial step of rice domestication. Because it has been important to increase the yield human had to select the rice varieties and species with low shattering degree. The shattering habit of rice is considered to be under the relatively simple genetic control compared with other characteristics related to domestication. Several recessive genes associated with the formation of an abscission layer, sh2, sh4 and sh-h on chromosomes 1, 3 and 7, have been reported. In addition, the grain shattering of rice is considered to be caused by seed abscission. The morphology of the abscission layer can differ in many different rice varieties that show varying degrees of shattering. Accordingly, it is important to elucidate the molecular mechanism to determine why some varieties do not have abscission layers and have an easy-shattering trait. In this study, analysis of QTL for grain shattering was performed to determine the location of QTLs on the whole chromosomes of rice. Also, we tried to construct a physical map for qPs6

Genetically modified (GM) plant claims to be the solution to global poverty, and potentially solving environmental change and food requirement by increased human population. In this study, we were evaluating agronomic characteristics and chemical properties of two GM drought-tolerant rice (CaMsrB2-8 and CaMsrB2-23) compared with donor cultivars (Ilmi). Statistical analysis agronomic characteristics GM and donor rice showed no significant difference between both of them. Yield and appearance of rice grain, GM rice was a similar to the donor rice. Chemical composition analysis showed that GM drought-tolerant rice has no different with donor rice. This result indicated that GM drought tolerant rice has no big significant difference agronomic character and chemical properties; it can be solve food shortages in spite of drought condition.

This experiment conducted to identify the changes of the response when white-back planthopper (WBPH, Sogatella furcifera), were inoculated in 10 days rice leaves after germination. We confirmed the difference between inoculated and uninfected plants by in the different time period (1day, 1 week, 2 weeks, and 3 weeks after inoculation). Breeding rice and WBPH maintained at 26∼28℃ with 60 % humidity. 3 leaves plants (TN1, Cheongcheong, and Nagdong) were inoculated with 2∼3 instars WBPH. Harvested rice plant samples were completely dried in dark condition and then samples were completely immersed in a solution of methanol for 3 days under darkness. Dissolving in water and then de-fatted three times with hexane. 100 ppm samples were applied to HPLC, eluting with acetonitrile and 0.1 % acetic acid by C18 (5ph column Agilant) and detected at 254 nm. We confirmed the difference of peak using LC/MS/MS (API-2000). The results showed that three weeks from the day of inoculation was increased at the molecular weight 118.1, 264.2 and 364.2.

본 연구는 대조군이 없고 실험기간이 짧아 연구 결과를 일반화하기에 제한점이 있으나 원예치료가 대안학교 학생들의 사회성과 스마일척도에 미치는 영향을 검증한 결과 유의함을 나타냈다. 이는 식물심기 등을 통해 공동체를 인식하여 사회성을 향상시키고 원예치료활동을 통해 긍정적 사고를 가지게 하여 스마일 척도를 향상시켰다고 생각된다. 따라서 사회에 적응하는데 어려움이 있고 부정적 사고가 많은 대안학교 학생들에게 건강한 인성발달을 위한 다양한 원예치료 프로그램의 개발이 필요하다고 사료된다.

Environmentally inflicted stress (abiotic stress) such as high drought stress could be limiting the plant productivity. The mechanism of drought stress signaling in plant related with anti-apoptosis has not yet been full described. Understanding drought stress signaling is key to producing drought-tolerant plant. In this study we recently have identified Oryza sativa genes related abiotic stress water deficit. Abiotic stress related genes were screened from Oryza sativa cDNA library and identified gene by yeast functional screening. The yeast expression showed that they east cell grow well on SD-galactose-Leu-Ura-. The screening of over than 7000 clones from Oryza sativa cDNA libraries has been identified. 28 clones that survived following BAX-expression on inducible galactose medium. R12H780 clones confirmed protein prediction like putative senescence-associated-protein. This gene contains an open reading frame (ORF) of 108 amino acids. Transcription of R12H780 was induced in response to drought stresses, RT-PCR analysis showed transcript level in plant strongly detected in earliest time of drought stress treatment. Yeast transformed with R12H780 gene displayed markedly improved tolerance to PEG treatment, and high salinity in comparison to the control yeast (vector only). The results indicate R12H780 expression represents a new type of drought stress related gene with anti-apoptotic in Oryza sativa and endows tolerance to several types abiotic stress.

The significance of genetic stability and bio-safety environment has been recently recognized by many GM plants. This study was to evaluate the GM stability of transgenic rice and to identify the environment variance. The GM rice of vitamin A　-enriched rice and four check cultivars were analyzed the data on agronomic characters and principal component for 2009-2011 in large-GM crop field. Cultivation environment was conducted in the large-GM field and greenhouse to determine grain characters. In this experiment, there was no significant difference in agronomic characters between GM rice of vitamin A-enriched rice and a donor plant, Nagdong. Related to grain characters, grain appearance and physicochemical characteristics were similar to GM rice of vitamin A-enriched rice and a donor plant, Nagdong. However, grain appearance in GM rice of vitamin A-enriched rice showed to white core and white belly when GM rice of vitamin A-enriched rice was planted in greenhouse. The type and distribution of dominant weed species also were not different from GM rice of vitamin A-enriched rice and a donor plant, Nagdong. Additionally that of gene flow was not detected in dominant weed species by PCR analysis.

This study was carried out to determine the contents of antioxidant activity of colored rice lines which derived from a mutant of MGI079 induced by MGI079 tissue culture in rice (Oryza sativa L.). The antioxidant activities were evaluated by assaying polyphenol, flavonoid, DPPH free radical and color values of colored rice lines, respectively. Among 8 lines including Heugnam of colored rice, the 70% ethanol extracts decreased in the order of MGI079-2-1>MGI079-2-6>MGI079-2-R,Heugnam>MGI079-1-R>MGI079-2-1>MGI079, MGI079-1-1, MGI079-1-6. The rice lines of highest polyphenolic compound was MGI079-2-6 and the next were Heugnam, MGI079-2-R, MGI079-2-1, MGI079-1-R, MGI079-1-6, MGI079-1-1, MGI079, MGI079-OP-R with the order of higher content. The flavonoid was higher in order of MGI079-2-1, MGI079-2-6, Heugnam, MGI079-2-R, MGI079-1-R, MGI079-1-6, MGI079-OP-R, MGI079, MGI079-1-1. The DPPH free radical was higher in order of MGI079-2-1, MGI079-2-6, Heugnam, MGI079-1-6, MGI079-1-1, MGI079-2-R, MGI079-1-R, MGI079-OP-R, MGI079. For chromaticity, a negative correlation was exhibited between the color value and the 70% ethanol extracts, polyphenolic compound, flavonoid, DPPH free radical. The grain characters in brown rice of a mutant of MGI079 showed similar to that of a donor plant, MGI079. Whereas, chemical characteristics of brown rice in two colored rice lines(MGI079-2-1, MGI079-2-6) were lower in amylose and lipid contents and were higher four times in zinc that of a donor plant, MGI079. Two colored rice lines(MGI079-2-1, MGI079-2-6) showed relatively high antioxidative activity in every results of antioxidant activity tests.

Somaclones regenerated from seed-derived callus culture of a rice cultivar, 'Ilpum', were evaluated for the agronomic and morphological characteristics. A total of 424 plants regenerated and the seeds collected from 297 lines were used for further analysis on morphological and agronomic traits. The results indicated that 21.5% (64 lines) of progenies showed significant differences from the donor cultivar 'Ilpum'. Somaclonal variations were induced leaf character, plant height, grain characters, heading date, and sterility. Among the variants, 8% (24 lines) of sterility, 1.4% (4 lines) of dwarf/semi dwarf, 1.7% (5 lines) of opaque, and 1.3% (4 lines) of leaf-rolling phenotypes were observed. The highest frequency of somaclonal variation was observed on heading date which occupied 9.1% (27 lines) of the entire lines included 6.4% (19 lines) of early heading and 2.7% (8 lines) of late heading. These results suggest that the somaclonal variation will be one of the promising genetic resources due to its wide variation and comparatively high frequency of mutation.

The whitebacked planthopper (WBPH) is one of the most serious insect pests of rice. The nymphs and adults suck the phloem sap which causes reduced plant vigor, stunting, yellowing of leaves. We employed a mapping population composed of 113 doubled haploid lines (DHLs), derived from a cross between resistant cultivar ‘Cheongcheong’ and susceptible cultivar ‘Nagdong’, to identify the quantitative trait loci (QTL) associated with WBPH resistance. The DH population was evaluated for resistance to WBPH using seed box screening test. The WBPH resistance of each lines or plants was estimated when susceptible check ‘Nagdong’ was killed using 1-9 scale according to Standard Evaluation System for Rice (IRRI, 1988). A linkage map was constructed with 119 polymorphic microsatellite markers using the Joinmap 4 software program to adjust for segregation distortion. Transgressive segregation was observed on WBPH resistance. Two significant QTLs conferring resistance to WBPH, qwbph6.1 and qwbph6.2, were detected in the regions of RM588-RM276 and RM527-RM528 on chromosome 6 with LOD scores of 3.7 and 2.4, respectively. They collectively explained 55% of phenotypic variation. These two QTLs have large effects on WBPH resistance and may be useful for establishment of marker assisted selection (MAS) in the rice breeding programs.