Background: Pluripotent stem cells (PSCs) including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) offer the immense therapeutic potential in stem cell-based therapy of degenerative disorders. However, clinical trials of human ESCs cause heavy ethical concerns. With the derivation of iPSCs established by reprogramming from adult somatic cells through the transgenic expression of transcription factors, this problems would be able to overcome. In the present study, we tried to differentiate porcine iPSCs (piPSCs) into endothelial cells (ECs) for stem cell-based therapy of vascular diseases. Methods: piPSCs (OSKMNL) were induced to differentiation into ECs in four differentiation media (APEL-2, APEL-2 + 50 ng/mL of VEGF, EBM-2, EBM-2 + 50 ng/ mL of VEGF) on cultured plates coated with matrigel® (1:40 dilution with DMEM/F-12 medium) for 8 days. Differentiation efficiency of these cells were exanimated using qRT-PCR, Immunocytochemistry, Western blotting and FACS. Results: As results, expressions of pluripotency-associated markers (OCT-3/4, SOX2 and NANOG) were higher observed in all porcine differentiated cells derived from piPSCs (OSKMNL) cultured in four differentiation media than piPSCs as the control, whereas endothelial-associated marker (CD-31) in the differentiated cells was not expressed. Conclusions: It can be seen that piPSCs (OSKMNL) were not suitable to differentiate into ECs in the four differentiation media unlike porcine epiblast stem cells (pEpiSCs). Therefore, it would be required to establish a suitable PSCs for differentiating into ECs for the treatment of cardiovascular diseases.

목적 : 본 연구는 국민건강영양조사 제7기(2016-2018) 원시자료를 이용하여, 녹내장에 영향을 미칠 수 요소 들과 그 연관성 정도를 파악하고자 하였다. 방법 : 녹내장 의사진단여부 및 만성질환에 응답한 만 30세 이상의 성인 총 5,244명을 대상으로, 녹내장에 영 향을 미칠 수 있는 신체활동과 만성질환 유병간의 기술통계 분석과 로지스틱회귀분석을 하였다. p<0.050인 경우 유의한 것으로 판단하였다. 결과 : 녹내장은 나이가 많아질수록 위험성이 높아지는 것을 알 수 있었다. 녹내장을 가지고 있는 사람의 신체 활동은 하루 중 앉아서 지내는 시간이 길고, 일주일에 근육운동을 하는 횟수가 많은 것으로 나타났다. 또한 변형 근로시간이 유의하게 많은 것으로 나타났다. 만성병과 관련하여서는 연령과 성별을 보정한 로지스틱회귀분석에서 이상지질혈증을 가지고 있는 사람들은 그렇지 않은 경우보다 녹내장의 발생 확률이 1.44배로 높아질 수 있는 것 으로 나타났다. 결론 : 본 연구는 녹내장 발병에 영향을 미치는 요인을 광범위하게 분석했다는 점에 의의가 있으며, 녹내장에 영향을 미칠 수 있는 생활 속 요인들을 파악함으로써 녹내장 발생 및 유병의 진행을 늦추는데 도움이 되고자 한다.

Zinc (Zn2+) is one of essential factors during mammalian oocyte maturation and fertilization. Previous studies showed that depletion of cellular Zn by metalion chelator impair asymmetric division of oocyte. But the detailed mechanism of these phenomena is unclear. We found that depletions of zinc by cell-permeable heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine (TPEN) caused the decrease of cytoplasmic actin mesh level. Spire2-GFP is co-localized with zinc at the cortex and intracellular vesicle. By the treatment of TPEN, number of Spire2-GFP decorated vesicle is drastically decreased, indicating that Zn2+is essential for the localization of the spire in mouse oocyte. Two putative zinc-binding regions were located in the C-terminal part of Spire2. Mutations of zinc binding site on spire abolish its localization at the intracellular vesicle. Over expression of C-terminal region containing zinc binding site of spire impair oocyte maturations and decrease cytoplasmic actin mesh. Taken together, these results suggest that intracellular zinc is crucial for the proper localizations of spire in the mouse oocyte, and unraveling the novel regulatory mode of actin nucleator spire by Zn2+.