sources of flavonoid, including quercetin, rutin and catechin. The flavonoid content of Tartary buckwheat is 9 - 300 times higher than the common buckwheat. The objective of this study was to compare phenolic compound content and antioxidant activity of tatary buckwheat and common buckwheat in different plant parts. Methods and Results : Total polyphenol, flavonoid contents and DPPH free radical scavenging activity was measured in seed, stem and leaf of tatary buckwheat followed by cultivation period. Total polyphenol content was higher in tatary buckwheat compared to common buckwheat. Total polyphenol contents was 347.33 ㎍/㎖ in tatary buckwheat seed extract and 57.24 ㎍/㎖ in common buckwheat stem (2 month). Total flavonoid content was also higher in tatary buckwheat than common buckwheat. Total flavonoid contents of tatary buckwheat seed extract was 45.68 ㎍/㎖, where as common buckwheat seed content 21.29 ㎍/㎖. DPPH free radical scavenging activity was higher in tatary buckwheat than common buckwheat. DPPH free radical scavenging activity was 85.76% in tatary buckwheat leaf (2 month) and 54.44% was in common buckwheat stem (2 month). Conclusion : This study indicates that polyphenol contents of tatary buckwheat extract depend on their part and the chronology is the seed> leaf > stem. Total polyphenol and flavonoid contents of leaf and stem is in inverse proportion to cultivation period. But, DPPH free radical scavenging activity make no difference according to plant cultivation age.

Background : Mistletoe grown in eurasian continent range from England to northern Asia. It has abundant chlorophyll and phenolic compound in node and leaf which has anticancer activity. The purpose of the study is to improve nutritional quality and composition of the amino acids and mineral along with increasing seed set rates and productivity of Viscum album on Prunus mume. Methods and Results : This study was conducted to compare plant nutrients such as total nitrogen, Ca, Fe, K, Mg, Mn, Na, P and composition of amimo acids in leaves between Viscum album and its host tree, Prunus mume, which were artificially cultivated in field and green house with or without treatment of fertilizer. Total nirogen content in leaves of mistletoe and its host tree, Prunus mume was 3.90% and 2.09% in the fields fertilized and non-fertilized, respectively. In a greenhouse fertilized, mistletoe leaves contained higher nitrogen (3.78%) compared to the host tree (3.06%). NO3-N content in leaves was also higher in mistletoe (37.5 ppm & 20 ppm) than in host tree (22.5 ppm & 12 ppm) in a field fertilized and non-fertilized. Ca content (1.18%) were same in leaves of mistletoe and host tree in the field fertilized. Contents of Fe, K, and Mn in leaves of mistletoe were 61.37 ㎎/㎏, 3.33%, and 127.59 ㎎/㎏, which were 16%, 13%, and 48% lower than host tree, respectively. On the other hand, contents of Mg, N, and P were 0.22%, 127.95 ㎎/㎏, and 0.48%, which were 5%, 148%, and 92% higher than host tree, respectively. K contents were higher in host tree fertilized (3.33% in field & 2.55% in greenhouse) while higher K (4.07%) was contained in a field non-fertilized compare to host tree (3.96%). However, P content was higher in mistletoe grown in field (0.48%) and greenhouse (0.42%) fertilized. In non-fertilized field, mistletoe leaves contained higher P (0.65%) rather than in host tree (0.29%). Eighteen kinds of amino acids were investigated in leaves of host plant by 20 - 87%. Conclusion : This study indicates that nutrient management including fertilization is important to improve nutritional quality and productivity of mistletoe in the artificial cultivation on Prunus mume.

The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water (90℃) treatment as a control. In our findings, highest TP (118 ㎎/g), TF (38 ㎎/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.