Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 ㎎ /g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 ㎎/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

Background : Bokbonja the Korean black raspberry (Rubus coreanus Miquel) fruit derived product, which is native to Korea. They contain potential anti-aging, anti-oxidants, depurative activities. Bokbonja refers to an immature berry of Rubus coreanus in the Korean pharmacopeia, Rubus chingii in Chinese pharmacopeia. Recently several Rubus species are available in the Korean drug markets which could easily find their way into drug prescriptions. Methods and Results : Hence we tried to detect the presence of these contaminating species in bokbonja products using SNP marker assisted by multi-plex PCR. We found a SNP region in the 26S region of these species and evaluated their potential to discriminate Rubus species. We designed a set of primer pairs such as, BokR primer has distinguished R.coreanus by producing a band at 852 bp and the primer sanF has differentiated R. crataegifolius by amplifying a band at 129 bp, while chiF has produced a band at 83 bp to distinguish R.chingii. These primer pairs effectively distinguished the bokbonja samples collected from various local markets as well as form drug store in Korea as well. The results were shocking as the bokbonja (R.coreanus) has a limited usage in Korea and either R. crataegifolius or R.chingii has been found in these samples. Taken together the primer pairs bokR, sanF, chiF along with 26S universal primers could effectively distinguish Rubus species in a single multiplex PCR reaction. Conclusion : Our results based on the 26S rDNA derived SNP region have shown that, the usage of R. coreanus is extremely limited and its often mistaken or adulterated with R. crataegifolius and R. chingii. It presses a panic situation in Korean peninsula to preserve bokbonja and the species R.coreanus which is endemic and superior in efficacy.