기존의 사후경과시간(PMI) 추정 방법은 대략적인 사후경과시간의 추정, 사후 48~72시간 경과 또는 부패 시 추정 불가능이라는 문제가 발생하여 최근에는 법의곤충학(Medicolegal entomology)을 통한 최소 사후 경과시간 (PMImin) 추정이 더욱 강조되고 있다. 이로 인해 시식성파리의 발육 성장속도 파악은 시체의 최소 사후 경과시간 추정에 있어 효과적인 도구로 활용될 수 있다. 본 연구에서는 곱슬털쉬파리(Parasarcophaga. similis)를 사육대상 종으로 선정하였다. P. similis는 부패한 시체를 섭식하는 시식성파리 중 하나로서, 발육 성장 속도에 따른 법곤충 학적(Forensic entomology) 최소 사후 경과시간을 추정하는 데 중요한 지표가 된다. 실험에 사용된 대상종의 경우, 경북 칠곡군 지천면 일대에서 6~9월경 야외쉬파리 채집하였고, 종 동정, 사육 및 증식, 예비 실험을 거친 후 본실험 을 진행하였다. 본실험은 산란 집단 케이지 내에서 산란 유도 후 12시간 간격으로 6개체를 샘플링하였고, 이를 Leica M125 Microscope (Leica Microsystems, Germany), EG-2HDNL(이지테크, Korea)로 이미지와 측정치를 확보 하였다. P. similis를 16~34°C 사이 7개의 온도 조건에서 성장 속도 측정을 3회 반복 실험하였으며, 곱슬털쉬파리의 최초 출현시점, 특정 온도 조건 별 발육 성장 속도 측정, 유충 몸길이 데이터, 이미지 자료, 표본 등을 확보하였다. 16, 19, 22, 25, 28, 31, and 34°C에서 P. similis의 알에서 성충까지의 발달 기간은 각각 822.0±5.9, 605.0±12.2, 442.3±9.4, 339.3±6.1, 289.3±7.5, 253.0±8.0, and 248.7±3.4h이 경과함을 확인하였다. 본 연구의 결과는 P. similis의 성장과정 데이터와 PMImin추정을 위한 지표로서 활용가능한 데이터를 제공한다.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen Post-thawed in boar. Recently, polymorphism (g. 35756 T>C) of Estrogen Receptor 1 (ESR1) gene reported to be significant association with MOT. This study was conducted to evaluate the ESR1 gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.35756 T>C SNP of ESR1 was significantly associated with frozen semen motility and kinematic characteristics. The g.35756 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.001). The SNP was also significantly associated with ALH (P<0.05). Therefore, we suggest that the g. 35756 T>C polymorphism in the intron 1 region of the porcine ESR1 gene could potentially be applied in frozen semen programs to improve MOT trait, but only after validation in other populations.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.158T>C) of phospholipase C zeta (PLCz) gene reported to be significant association with MOT. This study was conducted to evaluate the PLCz gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.158 T>C SNP of PLCz was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.0001, p= 0.0002, p<0.0001 and p<0.0001, respectively). Therefore, we suggest that the intron region of the porcine PLCz, may be used as a molecular marker for Duroc boar post-thawed semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.358A>T) of cluster-of-differentiation antigen 9 (CD9) gene reported to be significant association with MOT. Also, CD9 gene was expressed in the male germ line stem cells is crucial for sperm-egg fusion, and was therefore selected as candidate gene for boar semen. This study was conducted to evaluate the pig SNP (g.358A>T) of CD9 gene as a positional controlling for semen parameters of post-thawed boar semen. To results, the g.358A>T SNP of the CD9 gene was significantly associated with the traits such as MOT, curve linear velocity, straight line velocity, average path velocity and amplitude of lateral head displacement. Particularly, the g.358A>T SNP significantly has the highest association with MOT and animals with AA genotype (p<0.001). Therefore, we suggest that the g.358A>T in the intron 6 region of the porcine CD9 may be used as a molecular marker for Duroc boar Post-thawed semen quality, although its functional effect was not defined yet.