Silene fissipetala Turcz., which is native to Continental China and Taiwan, was newly found in Chungcheongnam-do, Korea. S. fissipetala is similar to the related taxa S. antirrhina L., S. armeria L., and S. koreana Kom. in that it has glutinous zones. However, S. fissipetala is distinguished from the congeneric species by the presence of laciniate at the petals. The species grows on slopes and roadsides, suggesting that it is likely to have been introduced through the installation of green sites and road construction. A precise description, photographs, voucher specimens and a key to related taxa are provided.

국립생물자원관에서는 자생생물 조사 및 발굴 사업을 포 함한 다양한 생물다양성 관련 사업들을 통해 표본과 생물소 재 등을 확보하고 있다. 기확보된 표본과 생물자원의 위치 정보에 근거한 자생생물의 정확한 분포 정보의 체계적인 관리 및 활용을 위하여 2014년 생물공간정보 서비스를 구 축하였다. 생물공간정보 서비스에서는 생물종 분포, 지역별 분포, 주제별 분포, 프로젝트별 그리고 채집자별 분포 정보 를 서비스하고 있다. 생물종 분포 메뉴에서는 하나의 생물종을 선택한 후 전국 또는 지역별 분포 지도를 조회할 수 있으며, 지역별 분포에 서 행정구역(시·도, 시·군·구), 생태경관보전지역, 국립공원 을 선택하여 해당 지역 내 분포하고 있는 생물종을 조회할 수 있도록 하였다. 행정구역에서는 광역시·도 및 시·군·구를 선택하여 분포 생물종을 조회할 수 있으며, 조사기간으로 시계열 조회가 가능하며, 고도검색, 버퍼검색 기능을 제공 하고 있다. 생태경관보전지역 검색기능에서는 거금도 적대 봉, 지리산, 동강유역 등의 생태경관보전지역 참조공간지도 를 적용하여 해당지역별 분포 생물종을 조회할 수 있도록 하였다. 또한, 21개 국립공원 참조공간지도를 적용하여 각 국립공원별로 분포 생물종을 조회할 수 있도록 하였다. 주제별 분포 메뉴에서는 기후변화지표생물종, 생태계교 란생물의 분포 정보를 제공하고 있다. 기후변화로 인해 계 절활동, 분포지역 및 개체군 크기 변화가 뚜렷하거나 뚜렷 할 것으로 예상되어 지표화하여 정부에서 지속적인 조사 및 관리가 필요한 생물종의 국내 분포를 조회할 수 있도록 하였다. 또한, 외국으로부터 유입된 종이거나 유전자변형생 물체 중 생태계의 균형에 교란을 가져오거나 가져올 우려가 있는 야생동물로써, 환경부령에 의해 현재 18종이 지정되어 있는 생태계교란생물에 대한 국내 분포를 조회할 수 있도록 구성되었다. 분포지도에는 4가지 주요 검색 기능과 2가지 다운로드 기능을 포함하고 있다. 첫 번째, 포인트/그리드 검색을 통해 표본 하나하나의 포인트를 표시하거나, 특정영역(그리드) 의 생물종 또는 표본점수의 통계를 조회할 수 있다. 연도별 검색에서는 특정연도 또는 기간을 선택하여 해당 기간의 분포지도를 조회할 수 있도록 구성하였다. 고도 검색에서는 특정 고도 범위내의 분포지도를 조회할 수 있도록 하였다. 강수량 검색에서는 특정 강수량 밤위 내의 분포지도를 조회 할 수 있도록 하였다. 또한, 화면상에 나타나는 분포지도를 이미지 파일로 다운로드하거나 분포지도에 나타나는 데이 터를 엑셀파일로 다운로드 할 수 있도록 하였다. 생물공간정보 서비스에서는 국가 생물자원 관리시스템 내 생물표본 및 유전자원 데이터베이스를 원시데이터로 사 용하고, 고도자료 및 기상청에서 제공하는 강수량 자료를 환경변수로 사용하고 있다. 배경지도로는 V-World 위성영 상 및 일반지도와 Open Street Map을 이용하며, 참조지도 로써, 행정구역도, 생태경관보전지역, 국립공원 위치도를 사용하였다. 국립생물자원관에서는 국가생물종목록 연번체계(KTSN) 를 이용하여 누구나 생물종 분포지도를 쉽게 연계하여 이용 할 수 있도록 서비스를 구축할 예정이며, 국가 생물자원 관 리시스템에 등록되는 모든 표본 정보에 대해 실시간으로 분포를 포함함으로써, 효율적이고, 체계적인 관리가 가능하 도록 시스템을 고도화 할 예정이다. 향후, 종 다양성 분석 등 연구관련 기능의 강화를 위해, 비교 지역에 대한 종다양도, 우점도, 유사도, 동일종 등을 분석 가능토록 할 계획이다.

This study was carried out to investigate the flora of Gyodongdo island (Ganghwa-gun). The vascular plants from 11 field surveys were revealed to belong to a total of 629 taxa; 118 families, 364 genera, 561 species, 5 subspecies, 53 varieties, 7 forms and 3 hybrids. 184 taxa were the first records from this region. The plants in Gyodongdo island are composed of the deciduous broad-leaved and conifer-mixed forests which are the common ones in the middle part of the Korean Peninsula. Five taxa of Korean endemic plants such as Clematis brachyura Maxim., Viola seoulensis Nakai, Populus × tomentiglandulosa T. B. Lee, Forsythia koreana (Rehder) Nakai and Hemerocallis hakuunensis Nakai were collected. Endangered wild plants designated by the law called 'Protection Law for Endangered wild fauna and flora' were one taxon. The red list plants according to IUCN valuation basis were examined for 13 taxa; endangered (EN) species of Prunus yedoensis Matsum., Vulnerable (VU) species of both Utricularia pilosa (Makino) Makino and Iris ruthenica var. nana Maxim., Near Threatened (NT) species of Senecio argunensis Turcz., Least Concern (LC) species of Platycladus orientalis (L.) Franco, Potentilla discolor Bunge, Limnophila sessiliflora (Vahl) Blume, Acorus calamus L., Phacelurus latifolius (Steud.) Ohwi, Pseudoraphis ukishiba Ohwi, Belamcanda chinensis (L.) DC., and Not Evaluate (NE) species of both Astragalus sikokianus Nakai and Potamogeton oxyphyllus Miq. The floristic regional indicator plants found in this area were a total of 47 taxa comprising three taxa of gradeⅤ, four taxa of grade Ⅳ, nine taxa of grade Ⅲ, 10 taxa of grade Ⅱ, and 21 taxa of gradeⅠ. The naturalized plants were identified as 62 taxa and the percentage of naturalized index (NI) was 9.9 % and the percentage of urbanization index (UI) was 19.3 %, respectively. Furthermore, hemicryptophytes (28 %), therophytes (26 %), hydrophytes (13 %) and geophyte (12 %) showed high proportional ratio in life form spectrum.

The studies on integrated operation of fuel cell with fuel processor are very essential prior to its commercialization. In this study, Polymer Electrolyte Membrane Fuel Cell (PEMFC) was operated with a fuel processor, which is mainly composed of two parts, methanol steam reforming reaction and preferential oxidation (PROX). In fuel processor, ICI 33-5 (CuO 50%, ZnO 33%, Al2O3 8%, BET surface area: 66 m2g-1) catalyst and CuO-CeO2 catalyst were used for methanol steam reforming, preferential oxidation (PROX) respectively. PEMFC was operated by hydrogen fuel generated from fuel processor. The resulting gas from PROX reactor is used to operate PEMFC equipped with our prepared anode and cathode catalyst. PtRu/C catalyst gives more tolerance to CO.

Randomly Amplified Polymorphic DNA (RAPD) was performed to define the genetic variation and relationships of Artemisia capillaris. Fifteen populations by the distributions and habitat were collected to conduct RAPD analysis. RAPD markers were observed mainly between 300bp and 1600bp. Total 72 scorable markers from 7 primers were applied to generate the genetic matrix, and 69 bands were polymorphic and only 3 bands were monomorphic. The genetic dissimilarity matrix by Nei's genetic distance (1972) and UPGMA phenogram were produced from the data matrix. Populations of Artemisia capillaris were clustered with high genetic affinities and cluster patterns were correlated with distributional patterns. Two big groups were clustered as southern area group and middle area group. The closest OTUs were GW2 and GG1 in middle area group, and GB1 from southern area group was clustered with OTUs in middle area group. RAPD data was useful to define the genetic variations and relationships of A. capillaris.

Genetic background and phylogenetic relationships among 20 Korean wheat cultivars were assessed using microsatellites after amplifying with 13 SSR primer pairs. Average allele number per primer pair was 3.36. Genetic similarities for every pair of cultivars ranged from 0.42 to 0.97, with 0.69 of overall average. Korean cultivars were divided into two major groups based on microsatellite DNA polymorphisms. Group I consisted of relatively old cultivars developed until 1970s, and group II contained the recent cultivars developed during 1980s and 1990s. Amongst old elite cultivars/lines, ‘Yukseung 3’, ‘Norin 12’ and ‘Norin 72’ contributed most to the genetic background of cultivars belonging to group I, and ‘Norin 4’, ‘Norin 12’, ‘Norin 43’ and ‘Norin 72’ to group II, respectively. The phylogenetic relationship of Korean wheat cultivars was in accordance with the genealogical data of each cultivar. The genetic background of each cultivar was assessed from the point of breeding and germplasm management such as variety identification and duplicated accessions for assisting in developing a system for the registration of new variety based on the molecular characterization in future.

Molecular markers are useful to confirm the hybridity of F1 plant derived from cross of two homozygous parents with similar morphological traits. RAPD markers were used to test F1 hybrid plant obtained from cross of two homozygous soybean (Glycine max) parents. Fl plant for cross I was made from the mating of Hobbit87 (female) and L63-1889 (male) and Fl plant for cross II was obtained from the mating of H1053 (female) and L63-1889 (male). Selfing plant per each cross was also obtained. Among 20 Operon primers used, OPA04 and OPA09 show polymorphism between cross I and II parent. Band in size 1Kb of OPA04 and 2.1Kb of OPA09 primer was polymorphic band. This fragment identified Fl hybrid plant and selfing plant in cross I and II. Female parent Hobbit87 in cross I and H1053 in cross II has no this fragment (recessive allele). However, male parent L63-1889 and Fl hybrid plant in cross I and II has this size of polymorphic band (dominant allele). This indicated that Fl hybrid and selfing plants were detected by RAPD marker before phenotypic marker would be used to identify Fl hybridity. Amplification products of selfing plant for cross I and II were completely same to the those of female parent. When mature, flower color of Fl hybrid plant in cross I and II was purple and flower color of selfing plant in cross I and II was white. Purple flower is dominant trait. Fl hybridity was successfully detected at very early growth stage using RAPD marker. Therefore, RAPD marker can be used broadly to confirm Fl hybridity in many crops.