양송이는 전 세계적으로 가장 많이 재배하는 버섯으로 특용작물생산실적의 2016년 통계자료에 의하면 국내 생산량은 10,173톤, 국내 생산액은 590억원이다. 현재 10개의 품종이 개발되었지만, 농업인들은 계속적으로 품질이 우수한 버섯 신품종을 요구하고 있다. 이에 우량계통을 육성하기 위해 수집된 170개의 유전자원 중에서 KMCC00754와 KMCC00775은 모균주로 선발되었다. 그리고 KMCC00754에서 120점의 단포자에서 동형핵균주로 추정되는 균주를 25점 선발하였고, KMCC00775에서는 120점의 단포자에서 6점을 AbSSR45마커를 가지고 각각 선발하였다. 이 동형핵균주로 교잡주를 육성하여 다시 SSR마커로 분석하였다. 그 결과 50%의 교잡율로 150점의 교잡주에서 74점이 교잡된 것을 확인하였으며, 이 균주들을 3회 재배하고 특성을 평가하였다. 최종적으로 Abs2-2015-16을 우량계통으로 선발하여 2017년에 신품종 ‘도담’이 육성되었다.

The global mushroom industry has grown rapidly in recent years in terms of beneficial effects, market value, and demand. India has a wide range of agro-climatic conditions and is largely an agricultural country with a cultivated area of about 4.37 %, generating about 620 million tons of agro waste annually. Mushroom cultivation not only helps recycle agro wastes, but also fills the nutritional gap prevalent among a large population of India. Recently, government industrial policy and creative innovation has promoted research and other endeavors aiming towards the cultivation of mushrooms. Mushroom cultivation in India was initiated in Solan, in the mid-sixties. Mushroom cultivation has been successful in temperate regions of the Himalayas, the Western Ghats, and the hills of northeast India. Recently, many unemployed people have begun to adopt mushroom cultivation as a means of self-employment. It is high time that Indian mushroom cultivators and consumers became aware of the nutritional and medicinal values of cultivated and wild species of mushrooms. The total mushroom production in India between 2010 and 2017 was approximately 0.13 million tons, accounting for a 4.3% increase in the average growth rate of mushrooms per annum. In particular, the total production of white button mushrooms is the highest, with a share of about 73% of total mushroom production. In this review article, we have analyzed the current scenario of the Indian mushroom industry and its contribution to the economic growth of the country.

발이가 안정적이고 다수성인 잎새버섯 신품종 『대박』의 주요특성은 다음과 같다. ‘대박’은 균사생장적온은 25℃로 ‘참’(대조품종)과 같았으며, 병재배 시 재배일수는 57일로 ‘참’에 비하여 2일이 짧았다. 발이율은 98.4%로 ‘참’에 비하여 24.8%p 높았으며, CV(변이계수)는 0.6%으로 ‘참’에 비하여 5.3%p 적었다. 갓색은 Hunter 색채값의 L값은 55.3으로 ‘참’에 비하여 3.6이 적어 갓색이 더 짙었으며, 갓두께는 1.73 mm으로 ‘참’에 비하여 0.53 mm 적었다. 자실체 직경은 132 mm로 ‘참’에 비하여 11 mm가 컸으며, 높이는 87.2 mm으로 ‘참’에 비하여 2.8 mm가 높았다. 1,100 ml 병당 자실체중량은 139 g으로 ‘참’에 비하여 28% 많았으며, CV는 2.5%로 ‘참’에 비하여 6.2%p가 적었다. 버섯재배농가의 일반적 1일 입병수 10,000병 기준으로 볼 때, 수량은 1,376 kg으로 ‘참’에 비하여 70% 높았으며, CV는 3.0%로 '참'에 비하여 11.5%p가 적었다. 물리성(강도 및 깨짐성)은 ‘참’에 비하여 낮았으며, 저장성은 판매가능기간으로 볼 때 4℃ 저장에서 42일로 ‘참’ 에 비하여 6일이 길었다.

Hypsizygus marmoreus is a mushroom with abundant flavor and medicinal properties. However, its application is limited by problems such as long cultivation period, low biological efficiency, and microbiological contamination; therefore, there is a substantial need for development of new cultivars of this species. In this study, 55 strains of H. marmoreus were subjected to inter simple sequence repeat (ISSR) analysis to identify markers for the selection of mother strains for breeding from the collected germplasm. ISSR 13 and 15 were confirmed as polymorphic markers. The three strains (KMCC03106, KMCC03107, and KMCC03108) with white cap color were found to be genetically closely related upon UPGMA analysis of both ISSR 13 and 15. Based on the PCR analysis results for ISSR 15, the collected germplasm were differentiated into three groups according to the strain collection year. Thus, ISSR 15 could be a marker for determining the phylogeny of cap color and genetic variations according to the strain collection year. These results suggest that ISSR markers can be effective tools for the selection of mother strains for breeding of H. marmoreus.

Flammulina velutipes belonging to white rot fungi is one of the commercially important edible mushrooms and is produced in large quantities due to the introduction of a automated and mechanized cultivation system in Korea. Despite the chief item of export among edible mushrooms, Flammulina velutipes has the lowest distribution rate of domestic cultivar, estimated that about 20 percent. As the result that most white cultivars of Flammulina velutipes produced and exported in Korea were introduced from Japan, farmers pay a large amount of royalties. Therefore, we try to develop a new pure domestic cultivars as a substitute for Japanese cultivars. To breed both white and gold superior strains, we selected the crossing mother groups including 10 white strains of ASI 4198 etc. and 7 brown strains of ASI 4049 etc. and mated each of the 17 strains by mon-mon hybridization. 19 white and 14 brown strains were chosen through two selection experiment over 2014 2016. In the third selection experiment this year, we finally selected one white(Fv 16 c 37) and the other gold(Fv 15 a 31) strain. Two selected strains were cultivated in the same environmental conditions. Spawn running period on the sawdust substrate required 30days at 20°C. The cultivation period and optimum temperature were 12±1 days at 14°C for primordia formation, 5 days at 4°C for inhibition phase, and 14±1 days at 7°C for fruiting body development. The length of pilei and stipes in two selected strains and Megumi as a control Japanese cultivar harvested in optimal stage was as follows: 10.5±0.81mm and 139.7±4.23mm in Fv 16 c 37, 10.8±0.43mm and 128..2±7.31mm in Fv 15 a 31, and 10.9±0.41mm and 141.8±4.64mm in Megumi respectively. The Yield of Fv 16 c 37, Fv 15 a 31 and Megumi was 271.2±11.84g, 237.7±9.05g and 270.7±16.87g per 1100ml in bottle cultivation.

Volvariella volvacea is mainly cultivated in subtropics area like South-East Asia. Because it is cultivated in rice straws, it is called a straw mushroom. That mushroom grows well in high temperature about 30~38°C and high humidity. Straw mushroom is a homothallic mushroom, so it is difficult to identify whether the offspring is different from the parents. This study was carried out to investigate RAPD primers that can be used for identification the DNA polymorphism of Volvariella volvacea genetic resources. 9 strains were collected from various countries like China, Vietnam etc. When ITS regions of their DNA were analyzed, they proved to be Volvariella volvacea. A cultivation test was conducted to measure the morphological characteristics of them 2 strains, KMCC04380 and KMCC04382 were selected for breeding resources because the mycelium of them grew well on medium and fruiting bodies were formed quickly. The Universal PCR Fingerprinting kits(UPF primers) were used to confirm the genetic polymorphisms of the 2 strains. As a result of confirming the DNA bands, 2 of 12 primers could be used to genetically distinguish 2 strains. About 50 spores were isolated from their fruiting bodies respectively and they also will be confirmed DNA polymorphisms by using UPF primers.

H. mamoreus is a mushroom with abundant favor and medicinal use. However, its cultivation has problems such as the long cultivation period, low biological efficiency and microbiological contamination, so new cultivars should be developed. In this study, 55 strains of H.marmoreus were analyzed with ISSR markers to identify precise genetic information in collected germplasm as mother strains in breeding. ISSR 13 and 15 markers were confirmed polymorphism. The three strains (KMC03106, KMC03107, and KMC03108) with white cap color were close genetic relationship in the UPGMA analysis of both the ISSR 13 and 15 markers. Especially in the PCR result of the ISSR 15, the collected germplasm were differentiated to three groups following collecting year. As these results, the ISSR 15 marker would be able to classify the phylogeny of cap color and genetic variation along the collecting year. Therefore ISSR markers will confer effective selection of mother strains for breeding of H. mamoreus.

The total mushroom production in Korea in 2015 comprised about 200,000 ton with a value of about 700 billion won (one trillion won if mushroom factory products are included, 1 $=1,128 won). This is almost equal to the amounts generated by flowers and medicinal crops. Even though the cultivation area and the number of farms have been decreased since 2006, the scale of the farms is increasing. Also mushroom productivity and consumption (4.9 ㎏ / person in 2015) are steadily increasing. Major cultivated species are Flammulina velutipes, Pleurotus ostreatus, Pleurotus eryngii, Lentinula edodes and Agaricus bisporus, which cover 90% of the total production. Shelves, logs, plastic bags or plastic bottles are mostly used for cultivation depending on the kind of mushroom. Mushroom export estimated about 43 million $ in 2016. Major exported mushrooms are Flammulina velutipes, Pleurotus eryngii, Lentinula edodes, Pleurotus ostreatus, Tricholoma matsutake and Agaricus bisporus that are destined for the U.S., the Netherlands, Australia, Canada, Hong Kong, Japan and several other countries. Mushroom research in the RDA started in 1967. In the beginning, development of cultivation methods using rice straw was studied together with distribution of improved strains of the button mushroom. The costs of button mushroom production strongly increased due the improving national economy, shortage of workers, increase of wages and the oil crisis in the nation. This caused a weak competitive position for button mushroom export and resulted in reduction of the button mushroom cultivation area. After development of a cotton waste cultivation method for the oyster mushroom (Pleurotus ostreastus), this mushroom was popularized and mainly studied. Development of automated plastic bottle cultivation systems in the 1990s changed shelf cultivation to mass production. The bottle system was applied to the oyster mushroom but also to F. velutipes and P. eryngii. Mushroom research in the RDA nowadays focuses on analysis of mushroom genetics in combination with development of new mushroom varieties, mushroom physiology and cultivation. Further studied are environmental factors for cultivation, disease control, development and utilization of mushroom substrate resources, post-harvest management and improvement of marketable traits. Finally, the RDA manages the collection, classification, identification and preservation of mushroom resources.

In Korea, oyster mushroom is one of the commonly cultivated mushrooms. In 2013, the cultivation areas and products of oyster mushrooms were 60,039 M/T and 201 ha, respectively. Many species of oyster mushrooms are cultivated in various countries. These include Pleurotus ostreatus, P. florida, P. sajor-caju, P. eryngii, P. citrinopileatus, P. salmoneostramineus and P. cystidiosus. P. citrinopileatus is a yellow oyster mushroom famous for its health benefits such as anti-cancer and anti-oxidant effects. Therefore, a cultivar P. citrinopileatus ‘Jangdari’ was developed to improve yield and the ability to grow well at lower temperatures. Two parent strains ‘Gumbit (KMCC02150)’ and ‘KMCC02145’ were selected based on their morphological characteristics. ‘Jangdari’ was developed by the method of Mon-Mon crossing between monokaryons derived from ‘Gumbit’ and ‘KMCC02145’, and finally selected through continuous cultivation tests. The optimum temperature for mycelial growth was 30oC. The cultivar could grow well at high temperatures, especially 16~24oC. Fruiting body production per bottle (850 mL) was about 90.0 g. Stipe length and thickness of ‘Jangdari’ were similar to those of ‘Gumbit’. ‘Jangdari’ was more resistant to low temperature than ‘Gumbit’, and thus it could be cultivated with oyster mushrooms (P. ostreatus). In addition, while cultivating ‘Jangdari’, it is not required to scrape out the upper side of bottle’s sawdust medium; hence, its cultivation is expected to save energy and time.

‘Baekseung’, a new variety of Flammulina velutipes, was bred by mating two monokaryotic strains isolated from KMCC 4210 and KMCC 4216 in Mushroom Research Division, Baekseung ARES in 2016. Baekseung showed fast mycelial growth and high mycelial density on MEA (Malt Extract Agar) media for 7days of incubation. Spawn running period on the sawdust substrate required 30days at 25°C. The cultivation period and optimum temperature were 11±1 days at 14°C for primordia formation and 14±1 days at 7°C for fruiting body development. The length of pilei and stipes in Baekseung harvested in optimal stage exhibited 11.3±0.4㎜ and 89.2±7.1㎜ and Megumi harvested in optimal stage showed 8.2±1.0㎜ and 95.9±5.0㎜ respectively. Yield of Baekseung and Megumi strain grown of sawdust substrate was 153.7±12.5g and 150.5±29.7g per 850ml in bottle cultivation. The inferred tree exhibited the difference of phylogenetic relationship between the Korean white fruiting body strains such as Baekseung, Uri1ho, Fv-14-a-38, and Fv-14-a-51 and the Japanese white fruiting body strain Megumi.

In this study, we made population with high biological efficiency to investigate the complex genetic architecture of yield-related traits in A. bisporus. MB013 that crossed with bisp 15-p2 and bisp 34-p2 had high biological efficiency. 170 homokaryons was isolated with CAPS marker (PIN primer/HaeⅢ) from 1000 ISSs. And 100 BC1F1 hybrids obtained by crossing the homokaryons of MB013 with bisp15-p1. Parental line bisp 15-p2 and bisp 34-p2 and 100 homokaryons of MB013 will analyze genome sequencing. Also 100 BC1F1 hybrids will evaluate yield-related traits.

Oyster mushrooms are widely cultivated and consumed in Korea. P. ostreatus 'Suhan(ASI 2504)' is an ideal cultivar for mushroom farmers due to its dark pileus and thick stipe; however, as it is very sensitive to environmental conditions, an alternative cultivar is required. To develop a new cultivar, parental strains 'Suhan(ASI 2504)' and ‘ASI 0665 (Heuktari)’ were selected from various collected strains according to morphological characteristics. P. ostreatus ‘Soltari’ was developed by Di- Mon crossing between the dikaryotic strain ‘Suhan’ and the monokaryotic strain derived from ‘Heuktari’. Thirty-eight of the 100 crossed strains were selected following analysis of mitochondrial genetic characteristics, and 'Soltari' was ultimately selected by continuous cultivation tests. The mitochondrial DNA profile of ‘Soltari’ was found to be the same as that of ‘Heuktari, and a nuclear DNA profile of ‘Soltari’ was similar as those of the parental strains, ‘Suhan’ and ‘Heuktari.’ 'Soltari' mycelium grows adequately in moderate to high temperatures of 12–20oC, although its optimum temperature was found to be 30oC. Fruiting body production per 1.1-L cultivation bottle was approximately 158.8 g. Its stipe length and thickness were comparable to those of diameter and thickness were somewhat lower (42.72 vs. 51.33 mm and 18.18 vs. 22.46 mm, respectively). ‘Soltari’ was found to be more resistant to high CO2 atmosphere than 'Suhan', and the color of the pileus of 'Soltari' was dark gray at high temperature. Therefore, it is suggested that this new cultivar ‘Soltari’ is a good alternative cultivar and will contribute to energy saving in oyster mushroom farms.

The production scale of mushrooms in Korea is approximately 600 billion won, which is 1.6% of Korea’s gross agricultural output. In Korea, ca. 190,000 tons of mushrooms are harvested annually. Although the numbers of mushroom farms and cultivators are constantly decreasing, total mushroom yields are increasing owing to large-scale cultivation facilities and automation. The recent expansion of the well-being trend has caused an increase in mushroom consumption in Korea: the annual per capita mushroom was 3.9 kg (’13), whichis a little higher than that in Europe. Thus, mushroom export, mainly Flammulina velutipes and Pleurotus ostreatus, has increased since the mid-2000s. Recently, however, it is slightly reduced. Nevertheless, Vietnam, Hong Kong, the United States, and the Netherlands continue to export mushrooms, and Korea has increased its export to Australia, Canada, Southeast Asia, etc. Canned Agaricus bisporus, the first export of the Korean mushroom industry, reached it speak sales in 1977-1978. When Korea initiated trade with China in 1980, the international prices of mushrooms fell sharply, leading to shrinkage of the domestic markets. Spurred by the high demand to develop substitute goods for A. bisporus, the oyster mushroom (P. ostreatus) gained attention since it seemed to suit the taste of Korean consumers. Although the log cultivation technique for oyster mushroom was developed in the early 1970s, it required a great deal of labor. Thus, we developed the shelf cultivation technique, which is easier to manage and allows for mass production. In this technique, the growing shelf is made mafrom fermented rice straw, whichis the only P. ostreatus medium in the world and isused only in South Korea. After then, the use of cotton wastes as an additional material of medium, the productivity. Currently, we are developing a standard cultivation technique and environmental control system that can stably produce mushrooms throughout the year. The increase of oyster mushroom production may boostthe domestic market and contribute to industrial development. In addition, oyster mushroom production technology played a role in forming the basis for the development of bottle cultivation, which made mass production . In particular, bottle cultivation using liquid spawn could allow for the export of F. velutipes and Pleurotus eryngii. In addition, the white varieties of F. velutipes were second developed in the world after Japan. We also developed the new A. bisporus cultivar ‘Saeah’, which is easy to grow in Korea. In hopes to advance the mushroom industry, we will continue to develop cultivars with international competitive power and to improve cultivation techniques.

Since the first Korean button mushroom ‘Sae-Ah’ developed in 2010, most farmers had wanted to cultivate a new variety. A new variety, ‘SaeJeong’, was developed in 2011, but this variety was difficult to cultivate in mushroom farm. To solve the variety problem, a new variety ‘Sae-do’ was developed in 2012. The variety, ‘Sad-do’, was made by crossing hetrokaryon A175 and homokaryon ASI1346-15 selected by genetic analysis. The heterokaryon A175 is crossed line between ASI1038-211 and ASI1346-20. The mycelium of ‘Saedo’ on CDA (compost dextrose agar) grew well at 25°C. The optimum pin-heading temperature of it was 13-15°C and optimum growing temperature was 13-20°C. Earliness (days required from casing to first harvesting) was 2 days shorter than control ‘Saejeong’. Even though the diameter of mature cap and the length of stipe were shorter than a control, the individual weight of fruiting body was heavier because of thick pileus and stipe. Therefore, the yield was increased by 1.89 times than control and the hardness of pileus was stronger. The field experiment of ‘Saedo’ variety has been started since 2013. Most farmers evaluated that the variety has good compost colonization and cap shape, and high yield in harvesting. For that reason, ‘Saedo’ has occupied half of the of distribution rate of Korean varieties. Also distribution rate of Korean varieties was increased from 26.1% in 2014 to 37% in 2015.

Oyster mushroom is one of mushrooms that are cultivated and consumed a lot in Korea. P. ostreatus 'ASI 2504(Suhan)' is a preferable cultivar to mushroom farmers because it has a dark pileus and a thick stipe. But it is very sensitive to environmental conditions, so farmers demand an alternative cultivar of ‘Suhan’ continuously. To develop a new cultivar, parental strains ‘ASI 2504(Suhan)’ and ‘ASI 0665(Heuktari)’ were selected from P. ostreatus’s various collected strains according to morphological characteristics. P. ostreatus ‘Soltari’ was developed by the method of Di-Mono crossing between dikaryotic strain ‘Suhan’ and monokaryotic strain derived from ‘Heuktari’. Analysis of the mitochondrial genetic characteristics was performed for primary selection in 100 crossed strains. The mitochondrial DNA profile of ‘Soltari’ was same as that of ‘Heuktari’, when mitochondrial DNA primer MtPo1 was used. And a nuclear DNA profile of ‘Soltari’ was similar as those of the parental strains, ‘Suhan’ and ‘Heuktari’, when RAPD(Random Amplified Polymorphic DNA) primer URP 1, 3 were used. The optimum temperature for mycelial growth was 30°C for ‘Soltari’. ‘Soltari’ was appropriate for middle high temperature to grow, especially 13~18°C. Fruiting body production per bottle (1,100mL) was about 158.6g. When compared to the control strain ‘Suhan’, the stipe’s length and thickness of ‘Soltari’ were similar to those of ’Suhan’. But the pileus diameter of ‘Soltari’ was a little shorter than that of ‘Suhan’, the former was 42.72mm, while the latter was 51.33mm. And the pileus thickness of ‘Soltari’ and ‘Suhan’ were 18.18mm and 25.46mm, respectively. ‘Soltari’ was more resistant at high CO2 concentration than ‘Suhan’ and the color of pileus of ‘Soltari’ was kept dark gray at high temperature. Therefore, it is suggested that this new cultivar ‘Soltari’ be an alternative of ‘Suhan’ and contribute to energy saving effect in oyster mushroom farms.

느타리 버섯류의 새로운 품종을 개발하기 위해 고품질 의 흑회색 느타리 신품종을 육성하였다. 2003년부터 2004년까지 느타리 유전자원의 특성을 검정하였고, 2008 년에 수한과 농기201호의 단핵체간 교잡하여 04-154 교 잡주를 육성하여 이를 이용해 구슬, 만추리, 야산을 육성 하였다. 2012년 구슬의 이핵체와 야산의 단핵체를 교잡하 여 몽돌을 육성하였고, 이 몽돌과 곤지7호를 교잡하여 갓 색깔이 우수한 Po 2012-706를 선발하여 특성검정, 확대 재배를 실시하여 농작물 직무육성 신품종 선정심의회에서 ‘고솔’로 명명하였다. 주요특성으로 균사 생장 적온이 25~30 o C이며 버섯 원기형성 및 발생온도는 12~18 o C이다. 자실체의 갓 색깔은 흑회색이며 자실체 형태는 옅은 깔때 기형이다. 대길이는 42.4±2.7 mm, 대굵기는 14.6±2.7 mm 로 수한에 비해 자실체 대가 가늘면서 긴 편이다. 자실체 수량은 병 당(850 mL) 124.2±35.2 g으로 수한이 100일 때 고솔은 116이었다. 가변특성으로는 감자배지와 버섯완전배지에서 균사를 배양한 결과 버섯완전배지에서 생장이 양호하였고 대조구 또한 같은 결과를 보였다. 4종류의 primer를 이용하여 새로운 품종 ‘고솔’과 모균주에 대한 DNA profile을 분석한 결과 primer URP1, primer URP2 에서 고솔이 양친의 주요 DNA 밴드를 갖고 있으며 대조 구인 ‘수한’과는 뚜렷하게 구분되었다. 신품종 느타리 ‘고 솔’은 소비자들이 선호하는 흑회색의 갓을 나타났고 저장 성이 좋아 고품질을 요구하는 소비자들을 만족시키는 데 기여할 것으로 기대된다.

Oyster mushroom is one of mushrooms that are cultivated and consumed a lot in Korea. P. ostreatus 'Suhan(ASI2504)' is a popluar cultivar because of high quality. But it is difficult for farmers to cultivate, so an alternative cultivar of ‘Suhan’ is demanded continuously. To develop a new variety, parental strains were selected using cultivation characteristics of Pleurotus ostreatus collected home and abroad. P. ostreatus 'Gosol' was developed by the method of Di-Mon crossing between dikaryotic strain P. ostreatus 'Mongdol(ASI0633)' and monokaryotic strain derived from P. ostreatus ‘Yasan(ASI0635)' in 2014. Analysis of the genetic characteristics of the new cultivar 'Gosol' showed a different DNA profile as that of the control strain, 'Suhan’ but a similar DNA profile as those of the parental strains, ‘Yasan’ and　'Mongdol’, when RAPD(Random Amplified Polymorphic DNA) primer URP 3 was used. The optimum temperature for mycelial growth was 25°C for ‘Gosol’ and ‘Suhan’. 'Gosol' was appropriate for middle high temperature to grow, especially 13~18°C. Fruiting body production per bottle was about 124.2g. When compared to the control strain 'Suhan’, the stipe was longer and the individual weight was heavier. But the stipe and the pileus were a little thinner than those of the control strain. ‘Gosol’ was more resistant at high CO2 concentration than the control strain. This new cultivar ‘Gosol’ of Pleurotus ostreatus was characterized dark bluish gray cultivar of oyster mushroom in the color of pileus and higer yield compared to those of other cultivar ‘Suhan’. Therefore, it is suggested that this new cultivar ‘Gosol’ be substitute for ‘Suhan’ in oyster mushroom’s farms.

The aim of this study was to analyze the genetic diversity of collected strains of Hypsizygus marmoreus based on their rDNA ITS sequences. The size of ITS rDNA regions of H. marmoreus strains, collected form various regions. A phylogenetic trees based on the ITS region revealed that the strains could be classified into 4 different groups including Villosiclava virens, Hypsizygus marmoreus, Lepista irina, Lyophyllum Decastes, Lyophyllum shimeji, Pleurotus floridanus.